Dynamics of dry- or fresh-weight of fruit, peel photosynthetic rate and chlorophyll content, and the characteristics of translocation and distribution of radiolabelled assimilates from leaf or fruit were examined in d...Dynamics of dry- or fresh-weight of fruit, peel photosynthetic rate and chlorophyll content, and the characteristics of translocation and distribution of radiolabelled assimilates from leaf or fruit were examined in developing satsuma mandarin (Citrus unshiu Marc. cv. Miyagawa wase) fruit from primary stage of fruit enlargement up to fruit full ripe. Change in fruit photosynthetic rate was some what related to the change in the chlorophyll content of peel. Fruit photosynthetic rate markedly declined as chlorophyll degradation occurred in the peel. Before full ripe stage of the fruit, photosynthates produced by a 14C-fed leaf were mainly distributed to juice sacs even during periods when dry matter accumulation in peel was more rapid than that in juice sacs. At the full ripe stage, peel photosynthetic rate approached zero and peel became the major sink of leaf photosynthates. Most of the peel assimilates, however, remained in situ for up to 48 h after feeding 14CO 2 to the fruit, only a small portion being transported to other parts of fruit. The percentage of fruit photosynthates exported decreased with fruit development and ripening, but the peak rate of export to juice sacs amount to as high as 12%. The sugar content and dry weights of peel and juice sacs in shaded fruit were lower than that in the control fruit. These results show that peel assimilate was mainly consumed in peel respiration and growth and thus the dependence on leaf photosynthates decreased. Part of this assimiate was used in sugar accumulation in juice sacs of fruit.展开更多
[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA...[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling.展开更多
Resurrection plants which are able to quickly reactivate after falling into a period of anabiosis caused by dehydration have been very rare among angiosperms, especially among dicotyledons whose chlorophyll content an...Resurrection plants which are able to quickly reactivate after falling into a period of anabiosis caused by dehydration have been very rare among angiosperms, especially among dicotyledons whose chlorophyll content and chloroplast structure little changed in the course of desiccation, therefore has been called homoiochlorophyllous desiccation-tolerant plants (HDTs). Another type of resurrection angiosperms that lost its chlorophyll dining desiccation is called poikilochlorophyllous desiccation-tolerant plants (PDTs). HDTs have been received more attention because of simplicity of protection mechanism which is much easy to the study and utilization of the desiccation tolerance of resurrection angiosperms. Recent advances in studies of photosynthesis of resurrection angiosperms indicate that photochemical activities are sensitive indicators for the study of physiological state of resurrection angiosperms during desiccation and rehydration. Photochemical activities of resurrection angiosperms are inhibited with loss of water similar to those of general plants, however, the magic thing is that they could reactivate rapidly during rehydration even losing more than 95% water. Up-regulations in xanthophyll cycle and antioxidative systems as well as preservation in integrity and stability of photosynthetic membranes during desiccation may be very important to desiccation tolerance of resurrection angiosperms. The fact that phosphate treatment in rehydration stage also strongly influences resurrection indicated importance of studies on rehydration stages of resurrection angiosperms.展开更多
Net photosynthesis ( P n ), transpiration ( E ), stomatal conductance ( g s) and water use efficiency (WUE) of more than 218 species belonging to two different reproductive functional types, i.e. clonal (115 ...Net photosynthesis ( P n ), transpiration ( E ), stomatal conductance ( g s) and water use efficiency (WUE) of more than 218 species belonging to two different reproductive functional types, i.e. clonal (115 species) and non_clonal species (103 species), along the 1 670 km Northeast China Transect (NECT) were analyzed. The results showed that P n and WUE appeared to be lower in the east and west ends of NECT, with peaks in the middle. Transpiration was found to be higher in the west end, where most temperate desert species were distributed. On the same site, most clonal species showed higher P n and related physiological variables than non_clonal species. For different growth forms over NECT, e.g. forest trees, shrubs and grasses, meadow steppe shrubs and grasses, typical steppe shrubs and grasses, the meadow steppe and typical steppe grasses, showed higher values of physiological variables than the forest or the desert species. But for the two reproductive plant functional types (PFTs), clonal species had higher physiological variables, with averages of 22%, 15%, 23% and 14% higher than the non_clonal ones for P n , E, g s, and WUE, respectively. Such differences indicated that clonal species might have advantages over non_clonal species in utilizing environmental resources such as light, CO 2, and especially water.展开更多
Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of pr...Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22 925 protein interaction pairs which involved 2 214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem Ⅰ (PSI), photosystem Ⅱ (PSII), light harvesting complex of photosystem Ⅰ (LHC Ⅰ) and light harvesting complex of photosystem Ⅰ (LHC Ⅱ) could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis.展开更多
Cyanobacteria are oxygenic photosynthetic Gram-negative bacteria that can form potentially toxic blooms in eutrophic and slow flowing aquatic ecosystems. Bloom toxicity varies spatially and temporally, but understandi...Cyanobacteria are oxygenic photosynthetic Gram-negative bacteria that can form potentially toxic blooms in eutrophic and slow flowing aquatic ecosystems. Bloom toxicity varies spatially and temporally, but understanding the mechanisms that drive these changes remains largely a mystery. Changes in bloom toxicity may result from changes in intracellular toxin pool sizes of cyanotoxins with differing molecular toxicities, and/or from changes in the cell concentrations of toxic and non-toxic cyanobacterial species or strains within bloom populations. We show here how first-order rate kinetics at the cellular level can be used to explain how environmental conditions drive changes in bloom toxicity at the ecological level. First order rate constants can be calculated for changes in cell concentration( μ_c : specific cell division rate) or the volumetric biomass concentration( μ_g : specific growth rate) between short time intervals throughout the cell cycle. Similar first order rate constants can be calculated for changes in nett volumetric cyanotoxin concentration( μ_(tox) : specific cyanotoxin production rate) over similar time intervals. How μ_c(or μ_g) covaries with μ tox over the cell cycle shows conclusively when cyanotoxins are being produced and metabolised, and how the toxicity of cells change in response to environment stressors. When μ_(tox)/μ_c >1, cyanotoxin cell quotas increase and individual cells become more toxic because the nett cyanotoxin production rate is higher than the cell division rate. When μ_(tox)/μ_c =1, cell cyanotoxin quotas remains fixed because the nett cyanotoxin production rate matches the cell division rate. When μ_(tox)/μ_c <1, the cyanotoxin cell quota decreases because either the nett cyanotoxin production rate is lower than the cell division rate, or metabolic breakdown and/or secretion of cyanotoxins is occurring. These fundamental equations describe cyanotoxin metabolism dynamics at the cellular level and provide the necessary physiological background to understand how environmental stressors drive changes in bloom toxicity.展开更多
The dinoflagellate Noctiluca scintillans is one of the most important and abundant red tide organisms and it is distributed world-wide. It occurs in two forms. Red Noctiluca is heterotrophic and fills the role of one ...The dinoflagellate Noctiluca scintillans is one of the most important and abundant red tide organisms and it is distributed world-wide. It occurs in two forms. Red Noctiluca is heterotrophic and fills the role of one of the microzooplankton grazers in the foodweb. In contrast, green Noctiluca contains a photosynthetic symbiont Pedinomonas noctilucae (a prasinophyte), but it also feeds on other plankton when the food supply is abundant. In this review, we document the global distribution of these two forms and include the first maps of their global distribution. Red Noctiluca occurs widely in the temperate to sub-tropical coastal regions of the world. It occurs over a wide temperature range of about 10℃ to 25℃ and at higher salinities (generally not in estuaries). It is particularly abundant in high productivity areas such as upwelling or eutrophic areas where diatoms dominate since they are its preferred food source. Green Noctiluca is much more restricted to a temperature range of 25℃-30℃ and mainly occurs in tropical waters of Southeast Asia, Bay of Bengal (east coast of India), in the eastern, western and northern Arabian Sea, the Red Sea, and recently it has become very abundant in the Gulf of Oman. Red and green Noctiluca do overlap in their distribution in the eastern, northern and western Arabian Sea with a seasonal shift from green Noctiluca in the cooler winter convective mixing, higher productivity season, to red Noctiluca in the more oligotrophic warmer summer season.展开更多
By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of...By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of the difference in Mn efficiency between wheat and oilseed rape. The results showed that there were significant differences in the abilities of MnIV reduction and acidification in root rhizosphere between the two species. Compared with wheat, oilseed rape had much higher reducing capacity and intensity of rhizosphere acidification under Mn-deficient conditions. Moreover, the higher ratio of functional leaves Mn/old leaves Mn in oilseed rape than in wheat was also an important factor for the different Mn efficiencies between the two species.展开更多
High performance liquid chromatography (HPLC) analysis of photosynthetic pigments in samples from Western Xiamen Bay and the Jiulong River Estuary showed that their major carotenoids were fucoxanthin, peridinin, zeaxa...High performance liquid chromatography (HPLC) analysis of photosynthetic pigments in samples from Western Xiamen Bay and the Jiulong River Estuary showed that their major carotenoids were fucoxanthin, peridinin, zeaxanthin, violaxanthin, and diadinoxanthin. Diatoms dominated in the spring bloom, dinoflagellates in summer. Violaxanthin and chlorophyll b indicated the appearance of chrysophytes and green algae, most of which were freshwater species. The high phytoplankton biomass region was located at the inner part of the bay. Diatoms and dinoflagellates dominated phytoplankton communities, contributing to over 50% of total biomass. All the main diagnostic carotenoids were significantly (P<0.01) correlated negatively to dissolved inorganic phosphorus, suggesting that they were limited by phosphorus. The high negative linear relationship (P<0.0001) between dissolved inorganic phosphorus and peridinin (dinoflagellates indicator) implied the potential of dissolved inorganic phosphorus for triggering red tide events in this region.展开更多
Pot-grown tomato plants (Lycopersicon esculentum Mill. cv. Maofen) was used to study the effects of three shading levels (0, 75% and 40%) for 8 days on dry matter partitioning, contents of nitrogen (N), phosphorus (P)...Pot-grown tomato plants (Lycopersicon esculentum Mill. cv. Maofen) was used to study the effects of three shading levels (0, 75% and 40%) for 8 days on dry matter partitioning, contents of nitrogen (N), phosphorus (P) and potassium (K) in leaves arid yield at three growth stages (early flowering (EF), peak flowering (PF) and later flowering (LF)). Shading reduced the dry weight of root and stem tissues at the EF and PF stages, but the 40% shading increased root dry weight and stem dry weight by 43.2% and 21.6%, respectively, at the LF stage. The influence of shading on the dry weight of leaves was very small at most growth stages. Shading had no effects on total leaf N, P and K contents at the EF and PF stages, showing that N, P and K absorption were regulated by the carbon assimilation at these two stages. The leaf N, P and K contents of 40% shaded plants at the LF stage were significantly increased. There were no obvious differences in leaf N and K contents between 75% and 40% shading treatments, but significant difference in leaf P contents was found between them at the LF stage. Shading significantly enhanced the fruit yield of 40% shaded tomato plants at the LF stage, but failed to affect the fruit yield of shaded plants at the EF stage. These showed that tomato could grow well and a better yield could be obtained if some moderate shading (i.e., 40% shading) was applied at the LF stage at summer midday.展开更多
Negative effect of precipitation on plant photosynthesis was investigated in this work. Stomatal conductance, transpiration rate and net photosynthetic rate were measured before and after each precipitation event, res...Negative effect of precipitation on plant photosynthesis was investigated in this work. Stomatal conductance, transpiration rate and net photosynthetic rate were measured before and after each precipitation event, respectively, and the corresponding precipitation was recorded as well. Moreover, plant dry matter accumulation was counted at the end of our entire experiment. The results show that precipitation fully demonstrates its negative effect on plant photosynthesis under the condition of without water shortage. Although it has not been proved, leaf shape seems to be associated with this effect. Broad-leaved species are less influenced than coniferous and lanceleaf species no matter on the length of variation time or changes in variation values. The different situation among three broad-leaved species seems to illustrate that the effect is also related to the size of single leaf area. The correlation between precipitation and photosynthetic rate variation is analogous to the relationship between precipitation and splash erosion, and in the view of the relationship between plant photosynthetic characteristics and dry mass accumulation, it can be thought that it can reflect the negative impact of precipitation on plant growth by making use of splash erosion. Therefore, a section was added in the traditional plant biomass estimation algorithms by using eco-physiological models, and this was proved to enhance the accuracy of traditional estimation from preliminary verifications.展开更多
The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultu...The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L^(-1)(high nitrogen, HN) and 2.9 m mol L^(-1)(low nitrogen, LN) of KNO_3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass(4.1 g L^(-1)). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid(TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids(NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L^(-1)d^(-1) was achieved in the HN group(on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase(on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L^(-1)d^(-1), respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions.展开更多
Fossil fuels are non-renewable, and their quantities have been reducing because of pumping. On the other hand, the traffic is still developing and needs for fuels are growing. As a consequence people are forced to loo...Fossil fuels are non-renewable, and their quantities have been reducing because of pumping. On the other hand, the traffic is still developing and needs for fuels are growing. As a consequence people are forced to look for other sources of getting fuel. Additional reason for this has been steadily boosting the prices of crude oil and gas. Diesel, as fuel that is often used, is possible to produce from different feedstock (oil rape, soybean, sunflower, palm, waste animal fats, algae, etc). In this paper, the author analyzes the possibility of obtaining biodiesel from algae and the feasibility of such a method of producing biodiesel. Algae for biodiesel production are analyzed and the systems in which they are growing are described. Experience in this area is described as well as opportunities for further development of technology for getting biodiesel from algae. Algae are very resistant and can grow virtually anywhere in the desert, in salt and fresh water and even in the waste water. Algae can reproduce quickly; they use C02 for photosynthesis and less water than other crops. Bio fuel from algae is biodegradable and contains no sulphur and it is not toxic.展开更多
Plants become photosynthetic through de-etiolation, a developmental process regulated by red/far-red light-absorbing phytochromes and blue/ultraviolet A light-absorbing cryptochromes. Genetic screens have identified i...Plants become photosynthetic through de-etiolation, a developmental process regulated by red/far-red light-absorbing phytochromes and blue/ultraviolet A light-absorbing cryptochromes. Genetic screens have identified in the last decade many far-red light signaling mutants and several red and blue light signaling mutants, suggesting the existence of distinct red, far-red, or blue light signaling pathways downstream of phytochromes and cryptochromes. However, genetic screens have also identified mutants with defective de-etiolation responses under multiple wavelengths. Thus, the opti- mal de-etiolation responses of a plant depend on coordination among the different light signaling pathways. This review intends to discuss several recently identified signaling components that have a potential role to integrate red, far-red, and blue light signalings. This review also highlights the recent discoveries on proteolytic degradation in the desensitization of light signal transmission, and the tight connection of light signaling with photoperiodic flowering and circadian rhythm. Studies on the controlling mechanisms of de-etiolation, photoperiodic flowering, and circadian rhythm have been the fascinating topics in Arabidopsis research. The knowledge obtained from Arabidopsis can be readily applied to food crops and ornamental species, and can be contributed to our general understanding of signal perception and transduction in all organisms.展开更多
A mercury (II) complex with 4.5-benzo-1.2-dithiol-3-thione (C7H4S3) (1), [Hg2I4(C7H4S3)2] (2) was synthesized. The structure of the obtained complex (2) was characterized by crystallography, Infrared Spect...A mercury (II) complex with 4.5-benzo-1.2-dithiol-3-thione (C7H4S3) (1), [Hg2I4(C7H4S3)2] (2) was synthesized. The structure of the obtained complex (2) was characterized by crystallography, Infrared Spectroscopy (IR) analysis and theoretical study. The complex (2) has a dimeric structure, where each mercury atom takes a tetrahedral geometry and is coordinated by two bridging chloride, one terminal chloride and one thiocarbonyl sulfur atom (C=S) of the ligand.展开更多
Fluorescence-based maximal photochemical efficiency, Fv/Fm, is widely used as an indicator to photosynthetic competence in marine systems. It has been considered a useful parameter diagnosing the nutrient stress on ph...Fluorescence-based maximal photochemical efficiency, Fv/Fm, is widely used as an indicator to photosynthetic competence in marine systems. It has been considered a useful parameter diagnosing the nutrient stress on phytoplankton photosynthesis, but many studies argue its usefulness. In the present study, we try to find a temporal relationship between Fv/Fm and nitrogen concentration, and provide a possible explanation on the controversy. We continuously measured Fv/Fm and nitrogen concentration once every 10 days from September 2003 to March 2004 at two stations in Jiaozhou Bay, northern China. It was found that Fv/ffm did not significantly correlate to synchronous nitrogen concentration, but the variation (i.e. the change between two adjacent cruises) of nitrogen concentration of the previous cruise and the variation ofFv/Fm of the current cruise were strongly correlated. This result indicates that a time lag exists between the variation of nutrient status and the subsequent Fv/Fm response. Length of the time lag seems just matched the interval of our measurements (10 days). In the field, direct dependence of Fv/Fm on nitrogen concentration may not be found because of the lagged response of Fv/Fm to nitrogen concentration variations or physiological acclimation. Our results provide a possible way to explain the previously reported conflicting results on the relationship between Fv/Fm and nutrient status. To give a more-accurate estimate about the length of the time lag, an investigation that includes more frequent measurements is needed.展开更多
PANI (polyaniline) as a promising conducting polymer and photosensitizer has been used to prepare PANI/TiO2 (polyaniline/TiO2) nanocomposite as photocatalyst. TiO2 nanoparticles with size of 5-100 nm were encapsul...PANI (polyaniline) as a promising conducting polymer and photosensitizer has been used to prepare PANI/TiO2 (polyaniline/TiO2) nanocomposite as photocatalyst. TiO2 nanoparticles with size of 5-100 nm were encapsulated by PANI via the "in situ" polymerization of aniline on the surface of TiO2 nanoparticles. IR, SEM, EPR techniques were used to characterize the mechanism of electron interaction in PANI/TiO2 nanocomposite. The resulting PANI-modified TiO2 composites exhibit significantly higher photocatalytic activity than that of neat PANI on degradation of MB (methylen blue) aqueous solution under UV irradiation.展开更多
文摘Dynamics of dry- or fresh-weight of fruit, peel photosynthetic rate and chlorophyll content, and the characteristics of translocation and distribution of radiolabelled assimilates from leaf or fruit were examined in developing satsuma mandarin (Citrus unshiu Marc. cv. Miyagawa wase) fruit from primary stage of fruit enlargement up to fruit full ripe. Change in fruit photosynthetic rate was some what related to the change in the chlorophyll content of peel. Fruit photosynthetic rate markedly declined as chlorophyll degradation occurred in the peel. Before full ripe stage of the fruit, photosynthates produced by a 14C-fed leaf were mainly distributed to juice sacs even during periods when dry matter accumulation in peel was more rapid than that in juice sacs. At the full ripe stage, peel photosynthetic rate approached zero and peel became the major sink of leaf photosynthates. Most of the peel assimilates, however, remained in situ for up to 48 h after feeding 14CO 2 to the fruit, only a small portion being transported to other parts of fruit. The percentage of fruit photosynthates exported decreased with fruit development and ripening, but the peak rate of export to juice sacs amount to as high as 12%. The sugar content and dry weights of peel and juice sacs in shaded fruit were lower than that in the control fruit. These results show that peel assimilate was mainly consumed in peel respiration and growth and thus the dependence on leaf photosynthates decreased. Part of this assimiate was used in sugar accumulation in juice sacs of fruit.
基金Supported by the Special Funds of Scientific and Technological Support Project of China(2009BADA8B01)Special Funds for Public Welfare Industry(Agriculture)Study of China(200903003)~~
文摘[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling.
文摘Resurrection plants which are able to quickly reactivate after falling into a period of anabiosis caused by dehydration have been very rare among angiosperms, especially among dicotyledons whose chlorophyll content and chloroplast structure little changed in the course of desiccation, therefore has been called homoiochlorophyllous desiccation-tolerant plants (HDTs). Another type of resurrection angiosperms that lost its chlorophyll dining desiccation is called poikilochlorophyllous desiccation-tolerant plants (PDTs). HDTs have been received more attention because of simplicity of protection mechanism which is much easy to the study and utilization of the desiccation tolerance of resurrection angiosperms. Recent advances in studies of photosynthesis of resurrection angiosperms indicate that photochemical activities are sensitive indicators for the study of physiological state of resurrection angiosperms during desiccation and rehydration. Photochemical activities of resurrection angiosperms are inhibited with loss of water similar to those of general plants, however, the magic thing is that they could reactivate rapidly during rehydration even losing more than 95% water. Up-regulations in xanthophyll cycle and antioxidative systems as well as preservation in integrity and stability of photosynthetic membranes during desiccation may be very important to desiccation tolerance of resurrection angiosperms. The fact that phosphate treatment in rehydration stage also strongly influences resurrection indicated importance of studies on rehydration stages of resurrection angiosperms.
文摘Net photosynthesis ( P n ), transpiration ( E ), stomatal conductance ( g s) and water use efficiency (WUE) of more than 218 species belonging to two different reproductive functional types, i.e. clonal (115 species) and non_clonal species (103 species), along the 1 670 km Northeast China Transect (NECT) were analyzed. The results showed that P n and WUE appeared to be lower in the east and west ends of NECT, with peaks in the middle. Transpiration was found to be higher in the west end, where most temperate desert species were distributed. On the same site, most clonal species showed higher P n and related physiological variables than non_clonal species. For different growth forms over NECT, e.g. forest trees, shrubs and grasses, meadow steppe shrubs and grasses, typical steppe shrubs and grasses, the meadow steppe and typical steppe grasses, showed higher values of physiological variables than the forest or the desert species. But for the two reproductive plant functional types (PFTs), clonal species had higher physiological variables, with averages of 22%, 15%, 23% and 14% higher than the non_clonal ones for P n , E, g s, and WUE, respectively. Such differences indicated that clonal species might have advantages over non_clonal species in utilizing environmental resources such as light, CO 2, and especially water.
基金Acknowledgements We thank the RIKEN BRC in Japan for provision of all full-length cDNA in this study. National Natural Science Foundation of China (grants numbers 30530100 and 90408010), the State Key Program of Basic Research of China (grant numbers 2007CB947600 and 2007CB108800), and Hi-Tech Research and Development Program of China (grant number 2006AA02Z313) supported this project.
文摘Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22 925 protein interaction pairs which involved 2 214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem Ⅰ (PSI), photosystem Ⅱ (PSII), light harvesting complex of photosystem Ⅰ (LHC Ⅰ) and light harvesting complex of photosystem Ⅰ (LHC Ⅱ) could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis.
文摘Cyanobacteria are oxygenic photosynthetic Gram-negative bacteria that can form potentially toxic blooms in eutrophic and slow flowing aquatic ecosystems. Bloom toxicity varies spatially and temporally, but understanding the mechanisms that drive these changes remains largely a mystery. Changes in bloom toxicity may result from changes in intracellular toxin pool sizes of cyanotoxins with differing molecular toxicities, and/or from changes in the cell concentrations of toxic and non-toxic cyanobacterial species or strains within bloom populations. We show here how first-order rate kinetics at the cellular level can be used to explain how environmental conditions drive changes in bloom toxicity at the ecological level. First order rate constants can be calculated for changes in cell concentration( μ_c : specific cell division rate) or the volumetric biomass concentration( μ_g : specific growth rate) between short time intervals throughout the cell cycle. Similar first order rate constants can be calculated for changes in nett volumetric cyanotoxin concentration( μ_(tox) : specific cyanotoxin production rate) over similar time intervals. How μ_c(or μ_g) covaries with μ tox over the cell cycle shows conclusively when cyanotoxins are being produced and metabolised, and how the toxicity of cells change in response to environment stressors. When μ_(tox)/μ_c >1, cyanotoxin cell quotas increase and individual cells become more toxic because the nett cyanotoxin production rate is higher than the cell division rate. When μ_(tox)/μ_c =1, cell cyanotoxin quotas remains fixed because the nett cyanotoxin production rate matches the cell division rate. When μ_(tox)/μ_c <1, the cyanotoxin cell quota decreases because either the nett cyanotoxin production rate is lower than the cell division rate, or metabolic breakdown and/or secretion of cyanotoxins is occurring. These fundamental equations describe cyanotoxin metabolism dynamics at the cellular level and provide the necessary physiological background to understand how environmental stressors drive changes in bloom toxicity.
基金the University Grants Council of Hong Kong and its Area of Excellence Program to PJH. KF was supported by a JSPS grant on the ecophysiology of green Noctiluca in the Gulf of Thailand. PMG received funding from NSF (No. OCE-1015980)This is contribution number 4502 from the University of Maryland Center for Environmental Studies. KY Acknowledges Support from the CAS/SAFEA International Partnership Program for Creative Research Teams (No. KZCXZYW-T001). DMA received partial funding through the NSF/NIEHS Centers for Oceans and Human Health (No. NIEHS P50 ES012742, NSF OCE- 043072 and OCE-0911031), and through NSF Grant (No. OCE-0850421)+1 种基金 This paper is based on work partially supported by SCOR/LOICZ Working Group 132, supported by the Scientific Committee on Oceanographic Research (SCOR) through grants from the U.S. National Science Foundation (No OCE-0938349 and OCE-0813697) from the Land-Ocean Interactions in the Coastal Zone (LOICZ) Project and the Chinese Academy of Sciences. We thank A. KANA for assistance with the GIS produced maps and LIU Hao for his assistance with the tables and references.
文摘The dinoflagellate Noctiluca scintillans is one of the most important and abundant red tide organisms and it is distributed world-wide. It occurs in two forms. Red Noctiluca is heterotrophic and fills the role of one of the microzooplankton grazers in the foodweb. In contrast, green Noctiluca contains a photosynthetic symbiont Pedinomonas noctilucae (a prasinophyte), but it also feeds on other plankton when the food supply is abundant. In this review, we document the global distribution of these two forms and include the first maps of their global distribution. Red Noctiluca occurs widely in the temperate to sub-tropical coastal regions of the world. It occurs over a wide temperature range of about 10℃ to 25℃ and at higher salinities (generally not in estuaries). It is particularly abundant in high productivity areas such as upwelling or eutrophic areas where diatoms dominate since they are its preferred food source. Green Noctiluca is much more restricted to a temperature range of 25℃-30℃ and mainly occurs in tropical waters of Southeast Asia, Bay of Bengal (east coast of India), in the eastern, western and northern Arabian Sea, the Red Sea, and recently it has become very abundant in the Gulf of Oman. Red and green Noctiluca do overlap in their distribution in the eastern, northern and western Arabian Sea with a seasonal shift from green Noctiluca in the cooler winter convective mixing, higher productivity season, to red Noctiluca in the more oligotrophic warmer summer season.
基金Project (No. 39425013) supported by the National Natural Science Foundation of China.
文摘By solution culture experiment, three wheat genotypes (Ttiticum aestivum L.) and two oilseed rape genotypes (Brassica napus L.) differing in Mn efficiency under Mn-deficient conditions were used to study mechanisms of the difference in Mn efficiency between wheat and oilseed rape. The results showed that there were significant differences in the abilities of MnIV reduction and acidification in root rhizosphere between the two species. Compared with wheat, oilseed rape had much higher reducing capacity and intensity of rhizosphere acidification under Mn-deficient conditions. Moreover, the higher ratio of functional leaves Mn/old leaves Mn in oilseed rape than in wheat was also an important factor for the different Mn efficiencies between the two species.
文摘High performance liquid chromatography (HPLC) analysis of photosynthetic pigments in samples from Western Xiamen Bay and the Jiulong River Estuary showed that their major carotenoids were fucoxanthin, peridinin, zeaxanthin, violaxanthin, and diadinoxanthin. Diatoms dominated in the spring bloom, dinoflagellates in summer. Violaxanthin and chlorophyll b indicated the appearance of chrysophytes and green algae, most of which were freshwater species. The high phytoplankton biomass region was located at the inner part of the bay. Diatoms and dinoflagellates dominated phytoplankton communities, contributing to over 50% of total biomass. All the main diagnostic carotenoids were significantly (P<0.01) correlated negatively to dissolved inorganic phosphorus, suggesting that they were limited by phosphorus. The high negative linear relationship (P<0.0001) between dissolved inorganic phosphorus and peridinin (dinoflagellates indicator) implied the potential of dissolved inorganic phosphorus for triggering red tide events in this region.
基金Project supported by the National Natural Science Foundation of China(No.40101005),the Provincial Natural Science Foundation of Shandong(No.Q2002E03)and the Provincial Education Committee of Shandong(No.J02L01).
文摘Pot-grown tomato plants (Lycopersicon esculentum Mill. cv. Maofen) was used to study the effects of three shading levels (0, 75% and 40%) for 8 days on dry matter partitioning, contents of nitrogen (N), phosphorus (P) and potassium (K) in leaves arid yield at three growth stages (early flowering (EF), peak flowering (PF) and later flowering (LF)). Shading reduced the dry weight of root and stem tissues at the EF and PF stages, but the 40% shading increased root dry weight and stem dry weight by 43.2% and 21.6%, respectively, at the LF stage. The influence of shading on the dry weight of leaves was very small at most growth stages. Shading had no effects on total leaf N, P and K contents at the EF and PF stages, showing that N, P and K absorption were regulated by the carbon assimilation at these two stages. The leaf N, P and K contents of 40% shaded plants at the LF stage were significantly increased. There were no obvious differences in leaf N and K contents between 75% and 40% shading treatments, but significant difference in leaf P contents was found between them at the LF stage. Shading significantly enhanced the fruit yield of 40% shaded tomato plants at the LF stage, but failed to affect the fruit yield of shaded plants at the EF stage. These showed that tomato could grow well and a better yield could be obtained if some moderate shading (i.e., 40% shading) was applied at the LF stage at summer midday.
基金Project(TD2011-01)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(20133050)supported by the China Scholarship Council
文摘Negative effect of precipitation on plant photosynthesis was investigated in this work. Stomatal conductance, transpiration rate and net photosynthetic rate were measured before and after each precipitation event, respectively, and the corresponding precipitation was recorded as well. Moreover, plant dry matter accumulation was counted at the end of our entire experiment. The results show that precipitation fully demonstrates its negative effect on plant photosynthesis under the condition of without water shortage. Although it has not been proved, leaf shape seems to be associated with this effect. Broad-leaved species are less influenced than coniferous and lanceleaf species no matter on the length of variation time or changes in variation values. The different situation among three broad-leaved species seems to illustrate that the effect is also related to the size of single leaf area. The correlation between precipitation and photosynthetic rate variation is analogous to the relationship between precipitation and splash erosion, and in the view of the relationship between plant photosynthetic characteristics and dry mass accumulation, it can be thought that it can reflect the negative impact of precipitation on plant growth by making use of splash erosion. Therefore, a section was added in the traditional plant biomass estimation algorithms by using eco-physiological models, and this was proved to enhance the accuracy of traditional estimation from preliminary verifications.
基金supported by the Natural Science Foundation of China (No. 31170337)the National High Technology Research and Development Program of China (863 Program) (No. 2013AA 065805)+1 种基金the National Basic Research Program of China (973 Program) (No. 2011CB2009001)the Special Program for Low-Carbon, Reform and Development Commission of Guangdong Province (No. 2011-051)
文摘The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L^(-1)(high nitrogen, HN) and 2.9 m mol L^(-1)(low nitrogen, LN) of KNO_3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass(4.1 g L^(-1)). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid(TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids(NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L^(-1)d^(-1) was achieved in the HN group(on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase(on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L^(-1)d^(-1), respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions.
文摘Fossil fuels are non-renewable, and their quantities have been reducing because of pumping. On the other hand, the traffic is still developing and needs for fuels are growing. As a consequence people are forced to look for other sources of getting fuel. Additional reason for this has been steadily boosting the prices of crude oil and gas. Diesel, as fuel that is often used, is possible to produce from different feedstock (oil rape, soybean, sunflower, palm, waste animal fats, algae, etc). In this paper, the author analyzes the possibility of obtaining biodiesel from algae and the feasibility of such a method of producing biodiesel. Algae for biodiesel production are analyzed and the systems in which they are growing are described. Experience in this area is described as well as opportunities for further development of technology for getting biodiesel from algae. Algae are very resistant and can grow virtually anywhere in the desert, in salt and fresh water and even in the waste water. Algae can reproduce quickly; they use C02 for photosynthesis and less water than other crops. Bio fuel from algae is biodegradable and contains no sulphur and it is not toxic.
文摘Plants become photosynthetic through de-etiolation, a developmental process regulated by red/far-red light-absorbing phytochromes and blue/ultraviolet A light-absorbing cryptochromes. Genetic screens have identified in the last decade many far-red light signaling mutants and several red and blue light signaling mutants, suggesting the existence of distinct red, far-red, or blue light signaling pathways downstream of phytochromes and cryptochromes. However, genetic screens have also identified mutants with defective de-etiolation responses under multiple wavelengths. Thus, the opti- mal de-etiolation responses of a plant depend on coordination among the different light signaling pathways. This review intends to discuss several recently identified signaling components that have a potential role to integrate red, far-red, and blue light signalings. This review also highlights the recent discoveries on proteolytic degradation in the desensitization of light signal transmission, and the tight connection of light signaling with photoperiodic flowering and circadian rhythm. Studies on the controlling mechanisms of de-etiolation, photoperiodic flowering, and circadian rhythm have been the fascinating topics in Arabidopsis research. The knowledge obtained from Arabidopsis can be readily applied to food crops and ornamental species, and can be contributed to our general understanding of signal perception and transduction in all organisms.
文摘A mercury (II) complex with 4.5-benzo-1.2-dithiol-3-thione (C7H4S3) (1), [Hg2I4(C7H4S3)2] (2) was synthesized. The structure of the obtained complex (2) was characterized by crystallography, Infrared Spectroscopy (IR) analysis and theoretical study. The complex (2) has a dimeric structure, where each mercury atom takes a tetrahedral geometry and is coordinated by two bridging chloride, one terminal chloride and one thiocarbonyl sulfur atom (C=S) of the ligand.
基金Supported by the Public Science and Technology Research Funds of Ocean(No.200905019)the Science Foundation of Young Scientist,SOA(No.2011129)+1 种基金the Outstanding Young & Middle Scientist of Shandong Province(No.BS2010HZ018)the Taishan Scholar Fund of Aquatic Animal Nutrition and Feed to ZHANG Limin
文摘Fluorescence-based maximal photochemical efficiency, Fv/Fm, is widely used as an indicator to photosynthetic competence in marine systems. It has been considered a useful parameter diagnosing the nutrient stress on phytoplankton photosynthesis, but many studies argue its usefulness. In the present study, we try to find a temporal relationship between Fv/Fm and nitrogen concentration, and provide a possible explanation on the controversy. We continuously measured Fv/Fm and nitrogen concentration once every 10 days from September 2003 to March 2004 at two stations in Jiaozhou Bay, northern China. It was found that Fv/ffm did not significantly correlate to synchronous nitrogen concentration, but the variation (i.e. the change between two adjacent cruises) of nitrogen concentration of the previous cruise and the variation ofFv/Fm of the current cruise were strongly correlated. This result indicates that a time lag exists between the variation of nutrient status and the subsequent Fv/Fm response. Length of the time lag seems just matched the interval of our measurements (10 days). In the field, direct dependence of Fv/Fm on nitrogen concentration may not be found because of the lagged response of Fv/Fm to nitrogen concentration variations or physiological acclimation. Our results provide a possible way to explain the previously reported conflicting results on the relationship between Fv/Fm and nutrient status. To give a more-accurate estimate about the length of the time lag, an investigation that includes more frequent measurements is needed.
文摘PANI (polyaniline) as a promising conducting polymer and photosensitizer has been used to prepare PANI/TiO2 (polyaniline/TiO2) nanocomposite as photocatalyst. TiO2 nanoparticles with size of 5-100 nm were encapsulated by PANI via the "in situ" polymerization of aniline on the surface of TiO2 nanoparticles. IR, SEM, EPR techniques were used to characterize the mechanism of electron interaction in PANI/TiO2 nanocomposite. The resulting PANI-modified TiO2 composites exhibit significantly higher photocatalytic activity than that of neat PANI on degradation of MB (methylen blue) aqueous solution under UV irradiation.