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光固定辣根过氧化酶修饰电极的直接电化学 被引量:2
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作者 高海燕 侯秀良 +4 位作者 许芳萍 刘仁 陶华 顾伟 汪茹 《光谱实验室》 CAS CSCD 北大核心 2009年第6期1679-1681,共3页
用聚氨酯丙烯酸酯预聚体将辣根过氧化物酶用光固定法制备了辣根过氧化物酶修饰电极。采用循环伏安法研究了该电极在没有媒介体的条件下的电化学性质,并考察了其对H2O2的生物电催化活性。结果表明,该电极可以在没有媒介体存在的条件下进... 用聚氨酯丙烯酸酯预聚体将辣根过氧化物酶用光固定法制备了辣根过氧化物酶修饰电极。采用循环伏安法研究了该电极在没有媒介体的条件下的电化学性质,并考察了其对H2O2的生物电催化活性。结果表明,该电极可以在没有媒介体存在的条件下进行直接而稳定的电子转移。扫描100圈后,扫描时的峰电流仍然几乎不变;在缓冲溶液中保存30天后,其伏安响应仍能保持保存前的约95%以上,且仍保持对H2O2电化学还原的生物电催化作用。这为酶电极的制备及酶的固定化提出了新方法。 展开更多
关键词 辣根过氧化物酶修饰电极 光固定法 直接电化学
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共固定化IFN-α和IFN-γ的抗宫颈癌作用 被引量:2
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作者 关燕清 温平香 +1 位作者 刘翠珊 周天鸿 《功能高分子学报》 CAS CSCD 北大核心 2005年第2期210-214,221,共6页
干扰素(Ⅰ型、Ⅱ型)是具有抗肿瘤作用的蛋白质型的细胞因子,与4叠氮苯甲酸反应,经红外光谱确认生成物在约2118cm-1处有叠氮基团的典型吸收,证明合成得到了光活性的干扰素。采用光固定法将光活性蛋白质固定到24孔组织培养聚苯乙烯板上,... 干扰素(Ⅰ型、Ⅱ型)是具有抗肿瘤作用的蛋白质型的细胞因子,与4叠氮苯甲酸反应,经红外光谱确认生成物在约2118cm-1处有叠氮基团的典型吸收,证明合成得到了光活性的干扰素。采用光固定法将光活性蛋白质固定到24孔组织培养聚苯乙烯板上,制成生物材料。实验通过扫描电子显微镜(SEM),对光固定化细胞因子的微观形态进行观察。通过做细胞生长曲线,透射电镜观察两种细胞因子固定化抑制宫颈癌细胞生长、诱导细胞凋亡的情况。实验表明,共固定IFNα和IFNγ能有效地抑制宫颈癌细胞的生长,在低剂量(60ng/孔)的情况下,抑制活性达到70%,而IFNγ具有明显的协同抑癌作用。 展开更多
关键词 干扰素 抗肿瘤作用 宫颈癌 光固定法 IFN-Α IFN—γ 生物材料
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Determination of Cholesterol Content in Waste Fat by Dual-wavelength Spectrophotometry
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作者 LIU Jia-di XU Shu-jun +1 位作者 XU Chun-xiang QIN Jin-ping 《Chinese Food Science》 2012年第2期40-42,共3页
[Objective]The aim was to find a way to measure the cholesterol content in deserted oil.[Method]Dual-wavelength spectrophotometry method was used for the determination of cholesterol in edible oil.630 nm and 720 nm we... [Objective]The aim was to find a way to measure the cholesterol content in deserted oil.[Method]Dual-wavelength spectrophotometry method was used for the determination of cholesterol in edible oil.630 nm and 720 nm were set as target band and reference band in this method,respectively.The result was compared to liquid chromatography method.[Result]The relative standard deviation(RSD)was at 1.26% and recovery was between 101.9% and 110.7%.Linear range was 8.0-40.0 μg/ml.The tested result was close to the result of liquid chromatography.[Conclusion]It was proved that this method was easy,fast and accurate.The disturbance of sitosterol was eliminated without isolation.It can be used for fast identification of the authenticity of waste fat. 展开更多
关键词 Dual-wavelength Spectrophotometry Waste fat CHOLESTEROL SITOSTEROL China
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Method of 3D Solar Energy Conversion
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作者 Valeri I. Kotelnikov Elena A. Ryazanova 《Journal of Mechanics Engineering and Automation》 2016年第4期212-216,共5页
The problem of collecting solar energy and increasing its efficiency was studied in this paper. It was discovered that a 3DPV (three-dimensional photovoltaic) structures can generate greater amounts of measured ener... The problem of collecting solar energy and increasing its efficiency was studied in this paper. It was discovered that a 3DPV (three-dimensional photovoltaic) structures can generate greater amounts of measured energy densities than stationary flat PV panels (rate: 2 to 20). It has been found that the same structures work better not only because they are made in 3D but because PV panels do not have linear dependency on geometry. It seems that the conversion efficiency depends on the process of absorption of the solar energy, too, or in other words on the E. Yablonovich limit. The findings suggest that the quantity of material of solar panels may be reduced to generate the same amount of electricity. 展开更多
关键词 Solar energy EFFICIENCY photovoltaic technology electricity generation energy transmission.
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