We investigated the radical scavenging effect and antioxidation property of polypeptide ex- tracted from Chlamys farreri (PCF) in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined th...We investigated the radical scavenging effect and antioxidation property of polypeptide ex- tracted from Chlamys farreri (PCF) in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined the scavenging effects of PCF on superoxide anions ( O?2 ), hydroxyl radicals (OH·), peroxynitrite (ONOO-) and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen free radicals including superoxide anions ( O2? ) (IC50 =0.3 mg/ml), hydroxyl radicals (OH·) (IC50 = 0.2 μg/ml) generated from the reaction systems and effectively inhibit the oxidative activity of ONOO- (IC50 = 0.2 mg/ml). At 1.25 mg/ml of PCF, the inhibi- tion ratio on lipid peroxidation of linoleic acid was 43 %. The scavenging effect of PCF on O?2 , OH· and ONOO- free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge free radicals and inhibit the peroxidation of linoleic acid in vitro. It is an anti- oxidant from marine products and potential for industrial production in future.展开更多
基金supported by National Natural Science Foundation of China(NSFC)under Grant No.61370090the Key Project of Chinese Ministry of Education under Grant No.211080+2 种基金the Talent Project of the Anhui Province for Outstanding Youth under Grant:2013SQRL064ZDthe Natural Science Foundation of Anhui Province under Grant No.1408085MA20the Key Program of Research Base of Hefei Normal University under Grant No.2012jd13
基金Supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao (No. 2001-28-50).
文摘We investigated the radical scavenging effect and antioxidation property of polypeptide ex- tracted from Chlamys farreri (PCF) in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined the scavenging effects of PCF on superoxide anions ( O?2 ), hydroxyl radicals (OH·), peroxynitrite (ONOO-) and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen free radicals including superoxide anions ( O2? ) (IC50 =0.3 mg/ml), hydroxyl radicals (OH·) (IC50 = 0.2 μg/ml) generated from the reaction systems and effectively inhibit the oxidative activity of ONOO- (IC50 = 0.2 mg/ml). At 1.25 mg/ml of PCF, the inhibi- tion ratio on lipid peroxidation of linoleic acid was 43 %. The scavenging effect of PCF on O?2 , OH· and ONOO- free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge free radicals and inhibit the peroxidation of linoleic acid in vitro. It is an anti- oxidant from marine products and potential for industrial production in future.