[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant mater...[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures of staining and mounting,the preparations were finished.When an anatomical digital photograph was processed,copy it into the word file and two copies of the original photograph were obtained.One copy was selected to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After it was copied into the word file,cut of the unnecessary parts and other operations were carried out,then processed photograph was obtained.[Results] The anatomical preparation for research was gotten.The analyzed digital photograph of the leaf structure of Salix matsudana var.matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantages of low cost and higher efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant anatomical digital photographs can acquire more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.展开更多
On the basis of fractional wavelet transform, we propose a new method called cascaded fractional wavelet transform to encrypt images. It has the virtues of fractional Fourier transform and wavelet transform. Fractiona...On the basis of fractional wavelet transform, we propose a new method called cascaded fractional wavelet transform to encrypt images. It has the virtues of fractional Fourier transform and wavelet transform. Fractional orders, standard focal lengths and scaling factors are its keys. Multistage fractional Fourier transforms can add the keys easily and strengthen information se-curity. This method can also realize partial encryption just as wavelet transform and fractional wavelet transform. Optical reali-zation of encryption and decryption is proposed. Computer simulations confirmed its possibility.展开更多
The comparative analysis of modem mathematical models for 3D problems in electron optics is presented. The new approach to solve the electron optics problems in three dimensions is presented. It is based on the princi...The comparative analysis of modem mathematical models for 3D problems in electron optics is presented. The new approach to solve the electron optics problems in three dimensions is presented. It is based on the principal ray method suggested by G. Grinberg in 1948. That perspective approach was not realized before for full three-dimensional electron optic systems, probably because of the complexity of its mathematical apparatus. We describe the analytical technique of the BEM （boundary element method） for the field evaluation, and 3rd order aberration expansion for the trajectory analysis. The first version of such computer code ＂OPTICS-3＂ and some results of numerical simulations with this code were presented.展开更多
基金Supported by National Natural Science Foundation of China(30770124)~~
文摘[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures of staining and mounting,the preparations were finished.When an anatomical digital photograph was processed,copy it into the word file and two copies of the original photograph were obtained.One copy was selected to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After it was copied into the word file,cut of the unnecessary parts and other operations were carried out,then processed photograph was obtained.[Results] The anatomical preparation for research was gotten.The analyzed digital photograph of the leaf structure of Salix matsudana var.matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantages of low cost and higher efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant anatomical digital photographs can acquire more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.
基金Project (No. 10276034) supported by the National Natural ScienceFoundation of China
文摘On the basis of fractional wavelet transform, we propose a new method called cascaded fractional wavelet transform to encrypt images. It has the virtues of fractional Fourier transform and wavelet transform. Fractional orders, standard focal lengths and scaling factors are its keys. Multistage fractional Fourier transforms can add the keys easily and strengthen information se-curity. This method can also realize partial encryption just as wavelet transform and fractional wavelet transform. Optical reali-zation of encryption and decryption is proposed. Computer simulations confirmed its possibility.
文摘The comparative analysis of modem mathematical models for 3D problems in electron optics is presented. The new approach to solve the electron optics problems in three dimensions is presented. It is based on the principal ray method suggested by G. Grinberg in 1948. That perspective approach was not realized before for full three-dimensional electron optic systems, probably because of the complexity of its mathematical apparatus. We describe the analytical technique of the BEM （boundary element method） for the field evaluation, and 3rd order aberration expansion for the trajectory analysis. The first version of such computer code ＂OPTICS-3＂ and some results of numerical simulations with this code were presented.
