In the face of deteriorating environmental conditions in the world,water quality control is an urgent task.It can be solved by creating sensors with high accuracy and low cost,which requires the development of fundame...In the face of deteriorating environmental conditions in the world,water quality control is an urgent task.It can be solved by creating sensors with high accuracy and low cost,which requires the development of fundamentally new radiophysical methods that take advantage of the optical,microwave and millimeter wavelengths that have a significantly greater sensitivity to low concentrations of pollutants and a lower inertia.The article presents prototypes of measuring cells of the microwave and optical ranges as well as the results of an experimental study of water of various degrees of pollution with their help.The results show that the use of the highly sensitive method of capillary-waveguide resonance makes it possible to detect the presence of micro impurities in water with concentrations up to0.1%and to identify water even from sources of various natural origins.In addition,the use of measurements at several frequencies in the optical range will make it possible to solve the problem of creating water control sensors with high sensitivity to pollution and low cost.It can be concluded that the possibility of complex use of multiwave sensors(optical,infrared and microwave ranges)allows to increase the sensitivity and reliability of water quality assessment.展开更多
Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it ...Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde （FQ） and fluorescein isothiocyanate （FITC） for the detection of rhEPO by capillary electrophoresis with laser-induced fluorescence detection （CE-LIF） were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the be used for the glycoforms profiling and quality control of rhEPO doping control analysis. glycoforms of rhEPO could be obtained. This method can It may be used as a candidate method for fast screening in展开更多
文摘In the face of deteriorating environmental conditions in the world,water quality control is an urgent task.It can be solved by creating sensors with high accuracy and low cost,which requires the development of fundamentally new radiophysical methods that take advantage of the optical,microwave and millimeter wavelengths that have a significantly greater sensitivity to low concentrations of pollutants and a lower inertia.The article presents prototypes of measuring cells of the microwave and optical ranges as well as the results of an experimental study of water of various degrees of pollution with their help.The results show that the use of the highly sensitive method of capillary-waveguide resonance makes it possible to detect the presence of micro impurities in water with concentrations up to0.1%and to identify water even from sources of various natural origins.In addition,the use of measurements at several frequencies in the optical range will make it possible to solve the problem of creating water control sensors with high sensitivity to pollution and low cost.It can be concluded that the possibility of complex use of multiwave sensors(optical,infrared and microwave ranges)allows to increase the sensitivity and reliability of water quality assessment.
基金National Natural Science Foundation of China(Grant No.20635001 and 21175005)
文摘Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde （FQ） and fluorescein isothiocyanate （FITC） for the detection of rhEPO by capillary electrophoresis with laser-induced fluorescence detection （CE-LIF） were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the be used for the glycoforms profiling and quality control of rhEPO doping control analysis. glycoforms of rhEPO could be obtained. This method can It may be used as a candidate method for fast screening in
