It is well known that optical tomography can accurately and quantitatively reconstruct the refractive index field of a transparent medium and display the three dimensional image of other physical quantities relevant t...It is well known that optical tomography can accurately and quantitatively reconstruct the refractive index field of a transparent medium and display the three dimensional image of other physical quantities relevant to temperature or density. In this paper, a new multidirectional holographic interferometric system is built, and two kinds of image reconstruction algorithms are introduced and an automatic image processing system of interferogram is designed. A three dimentsional asymmetric gas flow field above a combustor is expertmentally investigated with holographic interferometry. The reconstructed temperatures are similar to those measured with a thermocouple.展开更多
Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins.Rec...Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins.Recently,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of applications.Herein,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with light.The applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.展开更多
文摘It is well known that optical tomography can accurately and quantitatively reconstruct the refractive index field of a transparent medium and display the three dimensional image of other physical quantities relevant to temperature or density. In this paper, a new multidirectional holographic interferometric system is built, and two kinds of image reconstruction algorithms are introduced and an automatic image processing system of interferogram is designed. A three dimentsional asymmetric gas flow field above a combustor is expertmentally investigated with holographic interferometry. The reconstructed temperatures are similar to those measured with a thermocouple.
基金supported by the National Institutes of Health(GM-065978,AG-039253)
文摘Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins.Recently,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of applications.Herein,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with light.The applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.
