智能分光染色放大内镜在胃平坦型病变诊断中的应用价值

目的:对胃平坦型病变进行富士能智能分光染色内镜(Fujinon intelligent chromoendoscopy,FICE)放大内镜观察,对比FICE放大内镜与病理检查的一致性,探讨FICE放大内镜在胃平坦型病变诊断中的应用价值.方法:2012-09/2014-08对江汉大学附属医院发现的248个胃黏膜平坦性病变进行富士能FICE放大内镜检查.在FICE及放大模式观察病灶腺管开口与毛细血管形态,对其形态进行分型,并结合整体内镜下表现预测病理诊断.将FICE放大内镜下的内镜判定结果与病理组织学结果进行对比,评价其一致性与关联性.FICE内镜与病理诊断的一致性评价采用Kappa检验.结果:萎缩在FICE内镜下主要表现为C、D、E型胃小凹形态;肠上皮化生在F I C E内镜下主要表现为D、E型胃小凹形态;高级别上皮内瘤变及早期癌在FICE内镜下主要表现为E、F型胃小凹形态.FICE放大内镜技术判定萎缩、肠上皮化生、异型增生及早期癌的结果与病理诊断的结果具有较好的一致性.结论:FICE放大内镜技术有助于对病变性质如炎症、萎缩、肠上皮化生、上皮内瘤变及早期癌等的判断,有较好的临床应用价值.

富士能智能分光染色放大内镜联合小探头超声内镜在结直肠平坦及隆起病变中的诊断价值

[目的]探讨富士能智能分光染色放大内镜(FICE)联合小探头超声内镜(MPS)在结直肠平坦及隆起病变中的诊断价值。[方法]经结肠镜检出大肠黏膜平坦、隆起性病变患者93例,共发现≤2cm平坦、隆起病变数165个;所有病变均记录内镜下大体形态,后行FICE观察病变腺管开口形态及黏膜表面血管网形态,初步判断病变性质,再行MPS观察病变大小、来源、边界及回声性质等,进一步判断病变性质。比较单纯FICE检查、FICE联合MPS检查与病理诊断的一致性。[结果]FICE联合MPS判断结直肠平坦及隆起病变的良、恶性性质的灵敏度(91.4%)、准确率(88.5%)均高于单纯FICE,漏诊率及误诊率均低于后者,且与病理诊断具有较好的一致性(Kappa值=0.756)。[结论]FICE联合MPS检查对结直肠平坦及隆起病变的诊断价值较高,有利于判断病变性质及指导临床治疗方案的选择。

智能电子分光染色放大技术在大肠息肉诊断中的价值

染色内镜及放大内镜可鉴别肿瘤性及非肿瘤性息肉，并为临床选择治疗方案提供重要依据。但内镜下色素染色比较麻烦，操作较繁琐，耗时较长。新近开发的智能电子分光技术（FICE）是一种简单易行的黏膜表面观察方法，其采用纳米分光技术，模拟色素染色，可以再现黏膜表层细微结构及毛细血管走向。2007年1月至2011年1月间，我院采用FICE染色放大技术及色素染色放大技术对大肠息肉进行了腺管开口分型及毛细血管形态观察，并与病理组织学诊断相比较，现将结果报道如下。

复合分光染色内镜与普通内镜结肠息肉检出率的对比研究

目的比较复合分光染色技术(compound band imaging,CBI)与普通内镜检查对结肠息肉检出率的影响。方法选取解放军联勤保障部队第九○○医院2019年1月—2019年7月行结肠镜检查的患者,随机分为CBI组(在CBI模式下进行结肠镜检查)和白光组(进行常规结肠镜检查),比较两组患者息肉检出率。结果最终入组307例患者。CBI组患者结肠息肉总体检出率为44.2%(69/156),高于白光组的30.5%(46/151)(χ^2=6.208,P=0.013)。CBI组共检出息肉191枚,白光组共检出98枚,其中平坦型息肉(101枚比42枚)、非肿瘤性息肉(116枚比50枚)、直径≤5 mm息肉(99枚比41枚)、隆起型息肉(90枚比56枚)检出数量CBI组多于白光组,差异有统计学意义(P<0.05);肿瘤性息肉(75枚比48枚)、直径>5 mm息肉(92枚比57枚)检出数量两组差异无统计学意义(P≥0.05)。结论CBI可提高结肠息肉检出率,尤其是微小息肉和平坦型息肉,值得临床推广应用。

复合分光染色内镜在溃疡性结肠炎缓解期诊断中的应用研究

目的评价复合分光染色(compound band imaging,CBI)内镜在溃疡性结肠炎(ulcerative colitis,UC)缓解期判断黏膜愈合程度的诊断价值。方法纳入2017年1月至2019年12月在中国人民解放军联勤保障部队第九二八医院采用白光内镜进镜观察达到黏膜愈合的UC患者56例,随机采用白光模式(白光组28例)及CBI模式(CBI组28例)退镜观察,评估发现隐匿性黏膜炎症情况,同时对炎症部位行病理活检,采用Geboes评分进行炎症分级。结果退镜时间2组比较差异有统计学意义(χ^(2)=14.932,P=0.028)。CBI组发现9例部分肠段存在黏膜糜烂,病变局限于直肠2例,乙状结肠及直肠3例,左半结肠4例,Geboes评分≤3分2例,>3分7例,19例结肠黏膜正常;白光组未发现肠段黏膜糜烂,CBI组肠段黏膜糜烂发现率与白光组比较差异有统计学意义(χ^(2)=8.473,P=0.002)。结论CBI内镜可提高对UC缓解期隐匿性活动性炎症的识别能力,具有良好的临床应用价值。

FICE放大内镜联合乙酸染色对诊断胃黏膜肠上皮化生的价值

目的探讨富士能智能分光染色内镜(FICE)放大内镜联合乙酸染色对胃黏膜肠上皮化生(GIM)的诊断价值。方法疑似GIM患者480例随机分为普通内镜组、乙酸染色组、美蓝染色组、FICE放大内镜联合乙酸染色组各120例,以病理诊断为金标准,比较不同方法诊断肠上皮化生的差异。结果普通内镜组与乙酸染色组诊断GIM的灵敏度、特异度、准确率差异无统计学意义(P>0.05),美蓝染色组与乙酸染色组的灵敏度、准确率差异有统计学意义(P<0.05)。FICE放大内镜联合乙酸染色组与美蓝染色组的特异度、准确率差异有统计学意义(P<0.05);FICE放大内镜联合乙酸染色组的阳性似然比、优势比、Kappa值均明显高于美蓝染色组,阴性似然比则明显低于后者(P<0.05)。结论 FICE放大内镜联合乙酸染色可提高GIM定向活检准确率,显著降低漏诊率及误诊率,且与病理诊断的一致性较好,具有更高的临床诊断应用价值。

乙酸染色结合FICE内镜技术在胃黏膜肠上皮化生中的诊断价值

目的比较普通内镜、智能分光染色(FICE)内镜、FICE内镜结合乙酸染色在胃黏膜肠上皮化生检出中的诊断价值。方法从2012年12月至2014年12月,将我院普通内镜发现的可疑肠上皮化生病变患者分为3组各128例。分别采用普通内镜、FICE内镜、乙酸染色结合FICE内镜进行观察。比较不同方法诊断肠上皮化生的结果。结果 FICE内镜、FICE结合醋酸染色内镜诊断IM的敏感度、特异度优于普通内镜,差异有统计学意义(P<0.05)。FICE内镜与FICE结合醋酸染色内镜组间比较提示两者敏感度、总符合率、阴性预测值差异有统计学意义(P<0.05),但特异度、阳性预测值差异无统计学意义(P>0.05)。结论 FICE内镜、乙酸染色结合FICE内镜对肠上皮化生的诊断均优于普通内镜,乙酸联合FICE内镜较普通FICE内镜更能发现肠上皮化生病灶,减少漏诊,具有更高临床诊断价值。

ME-FICE联合超声小探头对早期胃癌及癌前病变的诊断价值研究

目的:探究放大智能电子分光染色技术(ME-FICE)联合超声小探头在早期胃癌及其癌前病变诊断中的应用价值。方法:选取2021年4月至2022年4月期间武汉市红十字会医院收治的80例疑似胃癌患者,使用ME-FICE区分阳性和(或)阴性,再使用高频超声小探头对病灶进行浸润深度探查。检查结束后在ME-FICE模式下对病灶明显处进行活检,其病检阳性者根据患者意愿对病灶行内窥镜下黏膜切除术(EMR)、内窥镜黏膜下剥离术(ESD)或外科手术切除,以术后病检结果为诊断“金标准”。采用Kappa一致性检验评价ME-FICE联合超声小探头与病检结果对良恶性诊断、内窥镜下浸润深度评估以及术后浸润深度评估的一致性。结果:ME-FICE联合超声小探头良恶性诊断的灵敏度、特异度及准确率分别为90.63%、79.17%和83.75%,均处于较高水平,Kappa值为0.887,与病例活检结果具有良好的一致性;ME-FICE联合超声小探头检查浸润程度评估的灵敏度、特异度和准确率分别为66.67%、90.00%和81.25%,灵敏度较差,其余处于较高水平,Kappa值为0.803,与术后病理结果具有良好的一致性。结论:ME-FICE联合超声小探头适用于早期胃癌及癌前病变的诊断,能够提高疾病诊断灵敏度及准确率,并能在术前进行较为准确的浸润程度评估,为后续手术选择和治疗提供指导。

性病性慢性前列腺炎患者T细胞亚群免疫荧光流式细胞分析

近年来,随着性传播疾病的流行,作为性病性尿道炎的合并症——性病性慢性前列腺炎也日益增多。本病治疗棘手,常迁延不愈。我们应用免疫荧光染色法和流式细胞仪定量检测性病性慢性前列腺炎患者外周血T细胞亚群,以探讨本病的发生发展与细胞免疫功能的关系。

微顺序注射-镉柱还原分光光度法测定海水中总氮

基于微顺序注射-阀上实验室,采用镉柱还原-偶氮染料染色分光光度法测定海水中总氮,对实验参数进行了优化,并进行了干扰因素实验。结果表明,海水中主要离子和盐度对本实验方法测定会产生干扰,采用一定盐度的国家标准海水作为溶剂制备系列标准溶液,可消除干扰。海水中总氮浓度在0.03~1.00 mg/L范围内与吸光度呈良好线性,线性相关系数r=0.9993;测定含氮浓度为0.2 mg/L的国家标准海水,相对标准偏差（RSD）为4.9%;方法的检出限为0.010 mg/L;样品加标回收率在99.5%~101.1%之间。经t检验分析,本方法与国标方法测定数据无显著性差异,可用于海水样品中总氮的测定。

皮肤经络的形态学基础及其立毛肌交感轴突反射传递机制

我们把经络分为血管性与非血管性两大类 ,本文深入研究了非血管性经络———皮肤信息通路或皮肤交感神经敏感线的结构和活动机制。首先用宏观放射自显影的方法 ,在大鼠皮肤中显示出纵贯全身的系列交感物质分布线 ,连续清晰、左右对称 ,在头部和肢体末端形成环路。在沿物质分布线经过的背上部切断皮肤 ,可以显著阻断针刺“足三里”产生的针刺效应 ,说明皮肤的交感物质分布线即针刺信号传递线路 ,或者说 ,经络的形态学基础已经显现出来。进一步通过SPG荧光染色法观察到 ,位于大鼠皮肤的交感物质分布线来源于其下支配立毛肌的交感神经网络。结合我们过去关于拔除经线毛囊阻断针刺效应的实验 ,提出毛囊立毛肌在经络实质中的动力靶器官作用和实体形态学基础。最后 ,为探索针刺信号的传递机制 ,将大鼠环形剪毛 ,针刺后出现与宏观显影线走行一致的立毛线或立毛带 ,表明交感物质分布线即立毛线。皮内注射苯肾上腺素或垂体后叶素后出现相似的立毛线 ;在去中枢神经支配的皮条上也观察到延伸至在体皮肤上的立毛线 ;全部切断皮肤阻断了立毛线的跨切口传递 ;仅切断部分真皮则没有影响 ,如果在切断部分真皮的切口内微量注射普鲁卡因同样可以阻断立毛线的跨切口传递。

流式细胞术外周血样品间标的方法

目的 建立一种新的用于流式细胞术 (FCM)外周血样品的间标荧光染色方法 .方法 抗凝全血 1 0 0 μL,加入一抗 5 0μL ,室温下反应 30 min,上 Q- PREP型免疫学样品制备仪 ,溶解红细胞和固定标品 ,加入荧光二抗 5 0 μL ,室温下反应 30 min,再加入 5 0 0 μL 的 PBS制成单细胞悬液 ,上流式细胞仪分析 ,同时与其他 5种常规方法进行比较 .结果 本方法与其他 5种方法的 FCM测定结果基本一致 (P>0 .0 5 ) ,而本方法样品用量少 ,操作简单 ,快速、染色时间仅需 70 m in.结论 我们建立的外周血样品间接荧光染色法 (方法 2 ) ,是外周血 FCM样品染色的理想方法 ,该方法对于 FCM在临床检验和基础研究的应用提供了技术支持 ,具有较强的实用价值 .

CBI内镜在早期胃癌诊断中的应用价值

背景:胃癌是我国常见的恶性肿瘤之一,早期诊断和早期治疗是提高患者生存率的关键。目的:评估复合分光染色(CBI)技术在早期胃癌内镜诊断中的应用价值。方法:2017年1月—2017年12月在上海交通大学医学院附属仁济医院行普通白光内镜检查发现局灶性胃黏膜病变的50例患者纳入研究,在国产CBI内镜下仔细观察病变黏膜腺管形态和微血管结构变化。以手术病理诊断结果为金标准,评估CBI内镜对早期胃癌的诊断效能。结果:50例病灶中29例在CBI模式下诊断为早期胃癌,21例为非早期胃癌。16例患者手术病理诊断为早期胃癌。CBI内镜诊断早期胃癌的敏感性、特异性和准确性分别为93. 8%(15/16)、82. 4%(28/34)和86. 0%(43/50),阳性预测值和阴性预测值分别为71. 4%(15/21)和96. 6%(28/29)。结论:国产CBI内镜对早期胃癌具有良好的诊断价值和临床应用前景,使在基层医疗机构开展早期胃癌筛查成为可能。

Hypertonic stimulation induces synthesis and release of glutamate in cultured rat hypothalamic astrocytes and C6 cells

Objective To investigate whether hypertonic saline （HS） can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and identified from the hypothalamus of newborn rat （1 day）. The astrocytes were randomly divided into five groups： isotonic （IS） and HS groups, astrocytes were incubated by IS and HS （320 mosM NaCl） medium, respectively, for 1, 3, 5, 10 or 15 rain; carbenoxolone （CBX） ＋IS and CBX＋HS groups, astrocytes were pre-treated with CBX （100 mmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, then removed to IS and HS medium, respectively, for 1, 3, 5, 10 or 15 min; Ca2＋＋HS group, astrocytes were pre-incubated with Ca2＋ （1 000 μmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, followed by a wash with isotonic FBS/DMEM, and then removed to hypertonic saline for 1, 3, 5, 10 or 15 min. The media of five groups were collected to analyze the medium glutamate concentration with high performance liquid chromatography. The astrocytes were fixed and double immunofluorescent stained with anti-glial fibrillary acidic protein （GFAP） and anti-glutamate. The C6 cells were divided into four groups： IS, HS, CBX＋IS and CBX＋HS groups, and used for quantitative measurement of glutamate in cells by flow cytometry （FCM）. Results （1） Anti-GFAP immunofluorescent signal revealed no significant difference among various time points in each group, or among the five groups. （2） The anti-glutamate immunofluorescent signal was increased in HS group and peaked at 5 min, and decreased and returned to the level of IS group at 15 rain （P 〈 0.01 vs the 5 min of HS group）. In CBX＋HS group, the glutamate intensity was higher than that in CBX＋IS and HS groups. （3） The medium glutamate concentration had no change after treatment with HS for 1 and 3 min, while increased markedly after treatment for 5 min to 15 min （P 〈 0.01 vs 1 min and 3 min）. On the contrary, the medium glutamate concentrations in the CBX＋HS or Ca2＋＋HS group were significant lower than that in the HS group （P 〈 0.01）. （4） FCM showed HS and CBX＋HS induced glutamate increase in C6 cells. Conclusion HS induced cultured rat hypothalamic astrocytes or C6 cells to synthesize and release glutamate; CBX could block glutamate release, but could not disrupt glutamate synthesis.

黄杨宁渗透泵型控释片的设计及释放度的研究

目的:以黄杨宁为模型药物制备黄杨宁渗透泵控释片。方法:采用酸性染料染色分光光度法对其释放度进行测定,考察不同处方下药物的释放情况。结果:制备的黄杨宁渗透泵控释片体外释药速度较平稳。结论:本制剂在14 h内呈现良好的零级释药特征,此后随渗透泵内药量的减少,释放速度有所下降。

The Self-incompatibility (S) Locus of Antirrhinum Resides in a Pericentromeric Region

The self-incompatibility ( S) loci from the Solanaceae, Rosaceae and Scrophulariaceae encode a class of ribonucleases, known as S RNases, which have been shown to control the pistil expression of self-incompatible reaction. In the former two families, the S loci have been shown to be located near centromere. However, the chromosomal location of the S locus in Antirrhinum, a species of the Scrophulariaceae, is not known. To determine its chromosomal location and genomic organization, an S-2 RNase gene and its corresponding 63 kb BAC clone were separately used for fluorescence in situ hybridization (FISH) of mitotic metaphase chromosomes of a self-incompatible Antirrhinum line Of S2S5. The results showed that the S-2 RNase detected a doublet signal near the centromere of the smallest chromosome (2n = 16). Two separate doublet signals of the tested BAC sequence were shown on both sides of the centromeres of all eight pairs of the chromosomes, suggesting that the Antirrhinum S locus is located in a pericentromeric region. Furthermore, a retrotransposon, named RIS1 (retrotransposon in the S locus), which has not been identified yet in. Antirrhinum, was found next to S-2 RNase. Taken together, the centromeric location of the S locus from the three S-RNase-based self-incompatible families provides a further support on a common origin of their evolution as well as suppressed recombination.

Effects of Homologous Genome 7 on Gas Exchange and Chlorophyll Fluorescence Parameters of Wheat

[Objective]To study the effects of homologous chromosomes 7 （7A,7B and 7D） on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochemical means. [Method]The Triticum asetivum cultivar Chinese Spring wheat and nullisomic wheat （N7A,N7B and N7D） were planted in greenhouse. The photosynthetic indexes were determined at early filling stage. [Result]The photosynthetic rate （Pn）,stomatal conductance （Gs）,primary photochemical efficiency （Fv/Fm）,actual chemical efficiency of photosystem II （ФPS II） and apparent electron transfer rate of photosystem II （ETR） were significantly lower in the N7A and N7B than in the Chinese Spring （P0.05）. The photosynthetic rate and stomatal conductance was significantly lower in the N7D than in the Chinese Spring （P0.05）. The Fv/Fm,ФPS II,ETR of N7D were higher than that of the Chinese Spring without significant difference. [Conclusion]The homologous chromosomes 7A and 7B have positive effects on photosynthetic rate and have relationship with stomatal conductance and photoreaction （chlorophyll fluorescence parameters）. The homologous chromosome 7D has negative effects on photosynthetic rate,which is mainly related to stomatal conductance rather than photoreaction.

Isolation and Chromosomal Mapping of a Corn B Chromosome Specific RAPDs

B染色体存在于多种动植物中 ,具有很多独特的性状。B染色体与正常染色体在DNA组成方面十分相似 ,寻找B染色体特异序列一直是B染色体研究的难点和热点。通过对含有和不含有B染色体的两种玉米 (ZeamaysL .)基因组进行了RAPD分析 ,筛选到一个B染色体特异性分子标记B480。该标记与玉米的自主复制起始序列ARS1和ARS2同源 ,特别是该序列中的 2 5bp出现在多种模式生物基因组中。FISH的结果显示 。

Blocking NF-kB nuclear translocation leads to p53-related autophagy activation and cell apoptosis

AIM: To investigate the anti-tumor effects of nuclear factor-κB (NF-κB) inhibitor SN50 and related mechanisms of SGC7901 human gastric carcinoma cells. METHODS: MTT assay was used to determine the cytotoxic effects of SN50 in gastric cancer cell line SGC7901. Hoechst 33258 staining was used to detect apoptosis morphological changes after SN50 treatment. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after SN50 treatment.Immunofluorescence staining was used to detect the expression of light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Western blotting analysis were used to determine the expression of proteins involved in apoptosis and autophagy including p53, p53 upregulated modulator of apoptosis (PUMA), damage-regulated autophagy modulator (DRAM), LC3 and Beclin 1. We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-α. RESULTS: The viability of SGC7901 cells was inhibited after SN50 treatment. Inductions in the expression of apoptotic protein p53 and PUMA as well as autophagic protein DRAM, LC3 and Beclin 1 were detected with Western blotting analysis. SN50-treated cells exhibited punctuate microtubule-associated protein 1 LC3 in immunoreactivity and MDC-labeled vesicles increased after treatment of SN50 by MDC staining. Collapse of mitochondrial membrane potential Δψ were detected for 6 to 24 h after SN50 treatment. SN50-induced increases in PUMA, DRAM, LC3 and Beclin 1 and cell death were blocked by the p53 specific inhibitor pifithrin-α. CONCLUSION: The anti-tumor activity of NF-κB inhibitors is associated with p53-mediated activation of autophagy.

Liraglutide directly protects cardiomyocytes against reperfusion injury possibly via modulation of intracellular calcium homeostasis

Background Liraglutide is glucagon-like peptide-1 receptor agonist for treating patients with type 2 diabetes mellitus. Our previous studies have demonstrated that liraglutide protects cardiac function through improving endothelial function in patients with acute myocardial infarction undergoing percutaneous coronary intervention. The present study will investigate whether liraglntide can perform direct protective effects on cardiomyocytes against reperfusion injury. Methods In vitro experiments were performed using H9C2 cells and neonatal rat ventricular cadiomyocytes undergoing simulative hypoxia/reoxygenation （H/R） induction. Cardiomyocytes apoptosis was detected by fluorescence TUNEL. Mitochondrial membrane potential （AWm） and intracellular reactive oxygen species （ROS） was assessed by JC-1 and DHE, respectively. Fura-2/AM was used to measure intracellular Ca2＋ concentration and calcium transient. Immtmofluorescence staining was used to assess the expression level of sarcoplasmic reticulum Ca2＋-ATPase （SERCA2a）. In vivo experiments, myocardial apoptosis and expression of SERCA2a were detected by colorimetric TUNEL and by immunofluorescence staining, respectively. Results In vitro liraglutide inhibited cardiomyotes apoptosis against H/R. △mψ of cardiomyocytes was higher in liraglntide group than H/R group. H/R increased ROS production in H9C2 cells which was attenuated by liraglutide. Liraglutide significantly lowered Ca2＋ overload and improved calcium transient compared with H/R group, lmmunofluorescence staining results showed liraglutide promoted SERCA2a expression which was decreased in H/R group. In ischemia/reperfusion rat hearts, apoptosis was significantly attenuated and SERCA2a expression was increased by liraglutide compared with H/R group. Conclusions Liraglutide can directly protect cardiomyocytes against reperfusion injury which is possibly through modulation of intracellular calcium homeostasis.