[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant mater...[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures of staining and mounting,the preparations were finished.When an anatomical digital photograph was processed,copy it into the word file and two copies of the original photograph were obtained.One copy was selected to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After it was copied into the word file,cut of the unnecessary parts and other operations were carried out,then processed photograph was obtained.[Results] The anatomical preparation for research was gotten.The analyzed digital photograph of the leaf structure of Salix matsudana var.matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantages of low cost and higher efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant anatomical digital photographs can acquire more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.展开更多
Color information plays a key role in the research fields of object recognition and image retrieval. However, the actual color varies by the conditions of illumination, especially the open natural daylight. Four diffe...Color information plays a key role in the research fields of object recognition and image retrieval. However, the actual color varies by the conditions of illumination, especially the open natural daylight. Four different color constancy schemes are proposed in the paper to minimize the effects of open illumination conditions. (1) The color constancy scheme based on the image statistics is proposed, which includes the color cast detection and removal. (2) The color constancy scheme based on the color temperature curve is proposed, which combines Gaussian model with linear fitting to estimate color temperature curve. (3) The color constancy scheme based on the double exposure theory is proposed, which is able to reproduce a color image under typical illumination. (4) According to the concepts of supervised learning, the supervised color constancy scheme is proposed. The transformation of color values from unknown illumination to typical illumination is solved by improved Support Vector Regression (SVR).展开更多
基金Supported by National Natural Science Foundation of China(30770124)~~
文摘[Objective] The research aimed to study a paraffin method without using the microtome,and also introduced an analysis method for optical information of the plant anatomical digital photographs.[Method] The plant material softened or not was embedded in paraffin according to the paraffin method.Cut the thin paraffin sections from the paraffin block with a sharp two-sided blade under anatomical lens.The thin material sections rolled up when they were cut off.Took the section rolls to a slide,and then heated them to melt the paraffin section roll.When the paraffin melted,the sections of plant material were rolled out.After the common or simplified procedures of staining and mounting,the preparations were finished.When an anatomical digital photograph was processed,copy it into the word file and two copies of the original photograph were obtained.One copy was selected to make it to be a negative photograph,and then press the key ＂Press Screen＂ to copy the screen frame.After it was copied into the word file,cut of the unnecessary parts and other operations were carried out,then processed photograph was obtained.[Results] The anatomical preparation for research was gotten.The analyzed digital photograph of the leaf structure of Salix matsudana var.matsudana f.tortuosa has some a three-dimensional effect,and the different leaf structures and cells,e.g.cuticle,cell wall,protoplast,vein,etc.can be identified easily.[Conclusion]The paraffin method without using the microtome has advantages of low cost and higher efficiency,which could be applied by the beginner or in the time without a microtome to be used.The analysis of the plant anatomical digital photographs can acquire more structural information than the original digital photographs,which shows the potentiality and prospects of the optical information analysis of the microscopic imagery and its digital photograph.
基金Supported by the National Natural Science Foundation of China (No.60431020)the Natural Science Foundation of Beijing (No.3052005)the Ph.D. Foundation of Ministry of Education (No.20040005015)
文摘Color information plays a key role in the research fields of object recognition and image retrieval. However, the actual color varies by the conditions of illumination, especially the open natural daylight. Four different color constancy schemes are proposed in the paper to minimize the effects of open illumination conditions. (1) The color constancy scheme based on the image statistics is proposed, which includes the color cast detection and removal. (2) The color constancy scheme based on the color temperature curve is proposed, which combines Gaussian model with linear fitting to estimate color temperature curve. (3) The color constancy scheme based on the double exposure theory is proposed, which is able to reproduce a color image under typical illumination. (4) According to the concepts of supervised learning, the supervised color constancy scheme is proposed. The transformation of color values from unknown illumination to typical illumination is solved by improved Support Vector Regression (SVR).
