In this report we illustrate our application of soft lithography-based microfabrication,surface modification,and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual ...In this report we illustrate our application of soft lithography-based microfabrication,surface modification,and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual forebrain neurons from Day 7 embryonic chicks into two rows of eight in a silicon microstructure aligned over a microelectrode array (MEA). The polydimethylsiloxane (PDMS) membrane with microstructures to confine cells and guide network connectivity was aligned to the electrodes of a MEA. Both the MEA and the PDMS membrane were treated with O2 plasma,Poly-L-Lysine,and Laminin to aid in cell attachment and survival. The primary advantage of our process is that it is quicker and simpler than previous cell-placement methods and may make highly defined neuronal network biochips more practical.展开更多
基金South Carolina Spinal Cord Injury Association (Grant No.SCIRF 0303)AHA (0565445U)+2 种基金NIH SC INBRE (Grant No.2p20RR16461-05)DoD Era of Hope Award (BC044778)NIH Career Award (1K25HL088262-01)
文摘In this report we illustrate our application of soft lithography-based microfabrication,surface modification,and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual forebrain neurons from Day 7 embryonic chicks into two rows of eight in a silicon microstructure aligned over a microelectrode array (MEA). The polydimethylsiloxane (PDMS) membrane with microstructures to confine cells and guide network connectivity was aligned to the electrodes of a MEA. Both the MEA and the PDMS membrane were treated with O2 plasma,Poly-L-Lysine,and Laminin to aid in cell attachment and survival. The primary advantage of our process is that it is quicker and simpler than previous cell-placement methods and may make highly defined neuronal network biochips more practical.
