利用试验生态学方法,研究3-羟基丁酸对雨生红球藻光系统Ⅱ(photosystemⅡ,PSⅡ)光化学活性与色素合成的影响。结果发现,3-羟基丁酸对雨生红球藻PSⅡ光反应中心造成胁迫损伤,细胞PSⅡ光化学最大光能转化效率(Fv/Fm)、有效光能转化效率(Yi...利用试验生态学方法,研究3-羟基丁酸对雨生红球藻光系统Ⅱ(photosystemⅡ,PSⅡ)光化学活性与色素合成的影响。结果发现,3-羟基丁酸对雨生红球藻PSⅡ光反应中心造成胁迫损伤,细胞PSⅡ光化学最大光能转化效率(Fv/Fm)、有效光能转化效率(Yield)和表观电子传递速率(electron transfer rate,ETR)的参数均出现不同程度降低,其中0.02、0.10μg/L处理组的作用最明显;3-羟基丁酸造成雨生红球藻叶绿素a含量波动变化并最终下降至较低水平;3-羟基丁酸能够促进雨生红球藻合成并积累虾青素,其中0.01μg/L浓度的促进作用最强,虾青素最高产量为7.57 mg/L;3-羟基丁酸影响了雨生红球藻自身资源在光合作用与抗逆合成之间的权衡分配。研究结果为使用3-羟基丁酸作为外源诱导物促进雨生红球藻生产虾青素提供了依据。展开更多
The structural and functional alterations within the PSⅡ membrane from phosphatidylcholine reconstitution and Triton X_100 (TX_100) treatment were studied by using Fourier transform_infrared (FT_IR) spectroscopic tec...The structural and functional alterations within the PSⅡ membrane from phosphatidylcholine reconstitution and Triton X_100 (TX_100) treatment were studied by using Fourier transform_infrared (FT_IR) spectroscopic technique and oxygen electrode. Phosphatidylcholine reconstitution showed no significant effect on the protein secondary structures of PSⅡ membrane but an increase of the rate of PSⅡ_mediated oxygen_evolution. The phosphatidylcholine lipids with different length of acyl chains displayed different capabilities to stimulate oxygen_evolution. In contrast, perturbation of the bilayer lipids by TX_100 resulted in obvious changes of the protein secondary structures within the PSⅡ membrane and in the loss of the PSⅡ_mediated oxygen_evolving activity. The results indicate the importance of membrane integrity in maintaining the stability of the photosynthetic membrane proteins.展开更多
The crystal of the title compound C17H22ClN3O3(Mr = 351.83) was prepared and its structure was determined by X-ray single-crystal diffraction. The crystal belongs to monoclinic, space group P21/n with the following cr...The crystal of the title compound C17H22ClN3O3(Mr = 351.83) was prepared and its structure was determined by X-ray single-crystal diffraction. The crystal belongs to monoclinic, space group P21/n with the following crystallographic parameters: a = 14.7641(1), b = 9.0050(7), c = 15.4874(1) ? =116.9590(1)? V = 1835.3(3) ?, Z = 4, Dx = 1.273 g/cm3, (MoK? = 0.227mm-1, F(000) = 744, the final R = 0.0516 and wR = 0.1594 for 2168 observed reflections. The X-ray data confirmed the z-sterochemistry of active cyanoacrylates and the presence of a planar core stablized by an intramolecular hydrogen bond between the ester carbonyl oxygen and the pyridyl methylamino hydrogen atom.展开更多
文摘利用试验生态学方法,研究3-羟基丁酸对雨生红球藻光系统Ⅱ(photosystemⅡ,PSⅡ)光化学活性与色素合成的影响。结果发现,3-羟基丁酸对雨生红球藻PSⅡ光反应中心造成胁迫损伤,细胞PSⅡ光化学最大光能转化效率(Fv/Fm)、有效光能转化效率(Yield)和表观电子传递速率(electron transfer rate,ETR)的参数均出现不同程度降低,其中0.02、0.10μg/L处理组的作用最明显;3-羟基丁酸造成雨生红球藻叶绿素a含量波动变化并最终下降至较低水平;3-羟基丁酸能够促进雨生红球藻合成并积累虾青素,其中0.01μg/L浓度的促进作用最强,虾青素最高产量为7.57 mg/L;3-羟基丁酸影响了雨生红球藻自身资源在光合作用与抗逆合成之间的权衡分配。研究结果为使用3-羟基丁酸作为外源诱导物促进雨生红球藻生产虾青素提供了依据。
文摘The structural and functional alterations within the PSⅡ membrane from phosphatidylcholine reconstitution and Triton X_100 (TX_100) treatment were studied by using Fourier transform_infrared (FT_IR) spectroscopic technique and oxygen electrode. Phosphatidylcholine reconstitution showed no significant effect on the protein secondary structures of PSⅡ membrane but an increase of the rate of PSⅡ_mediated oxygen_evolution. The phosphatidylcholine lipids with different length of acyl chains displayed different capabilities to stimulate oxygen_evolution. In contrast, perturbation of the bilayer lipids by TX_100 resulted in obvious changes of the protein secondary structures within the PSⅡ membrane and in the loss of the PSⅡ_mediated oxygen_evolving activity. The results indicate the importance of membrane integrity in maintaining the stability of the photosynthetic membrane proteins.
基金the National Natural Science Foundation of China(29832050)
文摘The crystal of the title compound C17H22ClN3O3(Mr = 351.83) was prepared and its structure was determined by X-ray single-crystal diffraction. The crystal belongs to monoclinic, space group P21/n with the following crystallographic parameters: a = 14.7641(1), b = 9.0050(7), c = 15.4874(1) ? =116.9590(1)? V = 1835.3(3) ?, Z = 4, Dx = 1.273 g/cm3, (MoK? = 0.227mm-1, F(000) = 744, the final R = 0.0516 and wR = 0.1594 for 2168 observed reflections. The X-ray data confirmed the z-sterochemistry of active cyanoacrylates and the presence of a planar core stablized by an intramolecular hydrogen bond between the ester carbonyl oxygen and the pyridyl methylamino hydrogen atom.