The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and inv...The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence 'burst' was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.展开更多
With the use of techniques in nonlinear problems, the IDP (improved discrepancy principle) method has been proposed and applied to the optimal smooth factor (parameterγ) in the inversion process of atmosphere profile...With the use of techniques in nonlinear problems, the IDP (improved discrepancy principle) method has been proposed and applied to the optimal smooth factor (parameterγ) in the inversion process of atmosphere profiles from satellite observation. This method has also been used to inverse atmospheric parameters from the observation of new generation geostationary operational environmental satellite (GOES-8). Results show that this method is more accurate than that in use.展开更多
Diffraction limited electron storage ring is considered a promising candidate for future light sources,whose main characteristics are higher brilliance,better transverse coherence and better stability.The challenge of...Diffraction limited electron storage ring is considered a promising candidate for future light sources,whose main characteristics are higher brilliance,better transverse coherence and better stability.The challenge of diffraction limited storage ring design is how to achieve the ultra low beam emittance with acceptable nonlinear performance.Effective linear and nonlinear parameter optimization methods based on Artificial Intelligence were developed for the storage ring physical design.As an example of application,partial physical design of HALS(Hefei Advanced Light Source),which is a diffraction limited VUV and soft X-ray light source,was introduced.Severe emittance growth due to the Intra Beam Scattering effect,which is the main obstacle to achieve ultra low emittance,was estimated quantitatively and possible cures were discussed.It is inspiring that better performance of diffraction limited storage ring can be achieved in principle with careful parameter optimization.展开更多
文摘The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence 'burst' was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.
文摘With the use of techniques in nonlinear problems, the IDP (improved discrepancy principle) method has been proposed and applied to the optimal smooth factor (parameterγ) in the inversion process of atmosphere profiles from satellite observation. This method has also been used to inverse atmospheric parameters from the observation of new generation geostationary operational environmental satellite (GOES-8). Results show that this method is more accurate than that in use.
文摘Diffraction limited electron storage ring is considered a promising candidate for future light sources,whose main characteristics are higher brilliance,better transverse coherence and better stability.The challenge of diffraction limited storage ring design is how to achieve the ultra low beam emittance with acceptable nonlinear performance.Effective linear and nonlinear parameter optimization methods based on Artificial Intelligence were developed for the storage ring physical design.As an example of application,partial physical design of HALS(Hefei Advanced Light Source),which is a diffraction limited VUV and soft X-ray light source,was introduced.Severe emittance growth due to the Intra Beam Scattering effect,which is the main obstacle to achieve ultra low emittance,was estimated quantitatively and possible cures were discussed.It is inspiring that better performance of diffraction limited storage ring can be achieved in principle with careful parameter optimization.
