Complementary DNA library of a dinoflagellate Pyrocystis lunula was constructed for the purpose of expression sequence tags analysis. The RNA isolated from this alga was about 20 μg g -1 net cells, and the band inten...Complementary DNA library of a dinoflagellate Pyrocystis lunula was constructed for the purpose of expression sequence tags analysis. The RNA isolated from this alga was about 20 μg g -1 net cells, and the band intensity ratio of 28 S/ 18 S in electrophoresis pattern was nearly 1 to 1. Different cDNA/vector molar ratios were exploited in the ligating reaction to be optimized. The clones produced by cDNA/vector molar ratio of 3.75 to 1 were desirable, most of whose inserts were longer than 300 bp. The recombinants insert length of the unfractionation cDNA library was largely shorter than 500 bp. However, in the fractionation library made from high molecule weight cDNA parts, over seventy percent of the recombinants contained inserts longer than 1 kb, some of which were even longer than 3 kb. Operating concerns were discussed at the end.展开更多
The meiotic process in Noctiluca scintillans were observed under light microscope. Some abnormal cell divisions, incompletely separated "zoospores" and the changes of the zoospores are described in this pape...The meiotic process in Noctiluca scintillans were observed under light microscope. Some abnormal cell divisions, incompletely separated "zoospores" and the changes of the zoospores are described in this paper. Together with the findings of field samplings and the previous results by other researchers, the process of meiosis in N. scintillans was supposed to be a pathway to reduce the extra high density of NH 3-N within the cell in order to ensure normal population growth.展开更多
OBJECTIVE: Bone mineral density (BMD) was measured in normal Chinese women with single X-ray absorptiometry (SXA) and dual energy X-ray absorptiometry (DEXA). These two methods were compared to evaluate the sensitivit...OBJECTIVE: Bone mineral density (BMD) was measured in normal Chinese women with single X-ray absorptiometry (SXA) and dual energy X-ray absorptiometry (DEXA). These two methods were compared to evaluate the sensitivity in reflecting bone loss. METHODS: Measurements were performed in 300 women aged 20 to approximately 79 (5 for each age). The 8mm distal, 1/4 distal, ultra distal site of the nondominant forearm were measured with SXA; the AP spine (L2-4), right femoral neck (Neck), Ward's triangle (Ward) and trochantor (Troch) were measured with DEXA. Ten women had 5 repeated measurements to evaluate the reproducibility of the equipments. RESULTS: The BMD peak for Neck and Ward was found between age 20 to approximately 29, for ultra distal and L2-4 at age 30 to approximately 39, for 8mm distal, 1/4 distal and Troch at age 40 to approximately 49. After reaching the peak values, BMD at all sites decreased with increasing age. The highest rate of yearly loss of BMD was at 8mm distal. Results of measurement showed significant correlation between SXA and DEXA. Comparing with premenopausal women, the bone loss rate in postmenopausal women was faster and highest up to 11 to approximately 15 years and the highest rate was at the Ward. CONCLUSION: The present study gives the normal values of BMD at seven sites for normal Chinese women. The ages of BMD peak were different at seven sites. The yearly loss of BMD was highest at 8mm distal, it seemed to be the sensitive region for bone loss with age. The postmenopausal women had highly significantly loss bone mass than the premenopausal women. Comparing with premenopause, postmenopause BMD for Ward's triangle with DEXA seemed to be most sensitive to the effect of menopause.展开更多
Objective： To identify radiographic predictors of residual low back pain （LBP） after laminectomy for lumbar canal stenosis Methods： （LCS） Clinical results and radiographic findings in 69 patients who underwent ...Objective： To identify radiographic predictors of residual low back pain （LBP） after laminectomy for lumbar canal stenosis Methods： （LCS） Clinical results and radiographic findings in 69 patients who underwent single level laminectomy for LCS were retrospectively reviewed. Patients who had an improvement in LBP scores evaluated by Japanese Orthopaedic Association （JOA） scoring system during the fol- low-up periods were classified as the recovery group, and others were classified as the non-recovery group. Patients＇ clinical data and radiographic parameters like lordosis angle, range of motion and intervertebral rotational angle were analyzed using binary logistic regression analysis to detect factors significantly related with the occurrence of residual LBP. Results： The average preoperative JOA score of 14.8± 5.05 improved to 21.59±5.51 at the final follow-up. Binary logistic regression analysis revealed that significant predictors of residual LBP were preoperative lumbar lordosis angle and range of motion. Conclusions： Our results suggest that patients with flat back and limited lumbar mobility before surgery tend to have poor results in terms of LBP. Therefore, these sagittal radiographic parameters should be taken into account when choosing laminectomy as the surgical option for LCS.展开更多
As the application of orbital angular momentum(OAM) of photon quantum in quantum communication, the OAM photon quantum interface for the transmission wavelength from the telecom communication quantum information stora...As the application of orbital angular momentum(OAM) of photon quantum in quantum communication, the OAM photon quantum interface for the transmission wavelength from the telecom communication quantum information storage in visible regime is required. Here we demonstrate the efficiency enhancement for the OAM photon quantum interface based on the frequency upconversion from telecom wavelength to visible regime by sum-frequency generation. The infrared photons at 1 558 nm carrying different OAM values could be converted to the visible regime at 622.2 nm with the optimal efficiency via adjusting the pump beam waist radius and intensity.展开更多
文摘Complementary DNA library of a dinoflagellate Pyrocystis lunula was constructed for the purpose of expression sequence tags analysis. The RNA isolated from this alga was about 20 μg g -1 net cells, and the band intensity ratio of 28 S/ 18 S in electrophoresis pattern was nearly 1 to 1. Different cDNA/vector molar ratios were exploited in the ligating reaction to be optimized. The clones produced by cDNA/vector molar ratio of 3.75 to 1 were desirable, most of whose inserts were longer than 300 bp. The recombinants insert length of the unfractionation cDNA library was largely shorter than 500 bp. However, in the fractionation library made from high molecule weight cDNA parts, over seventy percent of the recombinants contained inserts longer than 1 kb, some of which were even longer than 3 kb. Operating concerns were discussed at the end.
文摘The meiotic process in Noctiluca scintillans were observed under light microscope. Some abnormal cell divisions, incompletely separated "zoospores" and the changes of the zoospores are described in this paper. Together with the findings of field samplings and the previous results by other researchers, the process of meiosis in N. scintillans was supposed to be a pathway to reduce the extra high density of NH 3-N within the cell in order to ensure normal population growth.
文摘OBJECTIVE: Bone mineral density (BMD) was measured in normal Chinese women with single X-ray absorptiometry (SXA) and dual energy X-ray absorptiometry (DEXA). These two methods were compared to evaluate the sensitivity in reflecting bone loss. METHODS: Measurements were performed in 300 women aged 20 to approximately 79 (5 for each age). The 8mm distal, 1/4 distal, ultra distal site of the nondominant forearm were measured with SXA; the AP spine (L2-4), right femoral neck (Neck), Ward's triangle (Ward) and trochantor (Troch) were measured with DEXA. Ten women had 5 repeated measurements to evaluate the reproducibility of the equipments. RESULTS: The BMD peak for Neck and Ward was found between age 20 to approximately 29, for ultra distal and L2-4 at age 30 to approximately 39, for 8mm distal, 1/4 distal and Troch at age 40 to approximately 49. After reaching the peak values, BMD at all sites decreased with increasing age. The highest rate of yearly loss of BMD was at 8mm distal. Results of measurement showed significant correlation between SXA and DEXA. Comparing with premenopausal women, the bone loss rate in postmenopausal women was faster and highest up to 11 to approximately 15 years and the highest rate was at the Ward. CONCLUSION: The present study gives the normal values of BMD at seven sites for normal Chinese women. The ages of BMD peak were different at seven sites. The yearly loss of BMD was highest at 8mm distal, it seemed to be the sensitive region for bone loss with age. The postmenopausal women had highly significantly loss bone mass than the premenopausal women. Comparing with premenopause, postmenopause BMD for Ward's triangle with DEXA seemed to be most sensitive to the effect of menopause.
文摘Objective： To identify radiographic predictors of residual low back pain （LBP） after laminectomy for lumbar canal stenosis Methods： （LCS） Clinical results and radiographic findings in 69 patients who underwent single level laminectomy for LCS were retrospectively reviewed. Patients who had an improvement in LBP scores evaluated by Japanese Orthopaedic Association （JOA） scoring system during the fol- low-up periods were classified as the recovery group, and others were classified as the non-recovery group. Patients＇ clinical data and radiographic parameters like lordosis angle, range of motion and intervertebral rotational angle were analyzed using binary logistic regression analysis to detect factors significantly related with the occurrence of residual LBP. Results： The average preoperative JOA score of 14.8± 5.05 improved to 21.59±5.51 at the final follow-up. Binary logistic regression analysis revealed that significant predictors of residual LBP were preoperative lumbar lordosis angle and range of motion. Conclusions： Our results suggest that patients with flat back and limited lumbar mobility before surgery tend to have poor results in terms of LBP. Therefore, these sagittal radiographic parameters should be taken into account when choosing laminectomy as the surgical option for LCS.
基金supported by the National Natural Science Foundation of China(Nos.61378033 and 91021014)the Shuguang Program(No.15SG22)by Shanghai Education Development Foundation and Shanghai Municipal Education Commissionthe Open Project of State Key Laboratory of Precision Spectroscopy
文摘As the application of orbital angular momentum(OAM) of photon quantum in quantum communication, the OAM photon quantum interface for the transmission wavelength from the telecom communication quantum information storage in visible regime is required. Here we demonstrate the efficiency enhancement for the OAM photon quantum interface based on the frequency upconversion from telecom wavelength to visible regime by sum-frequency generation. The infrared photons at 1 558 nm carrying different OAM values could be converted to the visible regime at 622.2 nm with the optimal efficiency via adjusting the pump beam waist radius and intensity.
