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胃食管反流病患者食管上皮Occludin的表达及细胞间隙的改变 被引量:7
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作者 徐兆军 张振玉 +3 位作者 黄文斌 王劲松 姜宗丹 罗新华 《世界华人消化杂志》 CAS 北大核心 2009年第27期2835-2839,共5页
目的:研究胃食管反流病(GERD)患者食管上皮细胞紧密连接蛋白Occludin表达的变化及食管上皮细胞间隙的改变.方法:采用免疫组织化学法检测62例GERD患者[其中反流性食管炎(RE组)患者30例,非糜烂性反流病(NERD组)患者32例]及10例正常对照食... 目的:研究胃食管反流病(GERD)患者食管上皮细胞紧密连接蛋白Occludin表达的变化及食管上皮细胞间隙的改变.方法:采用免疫组织化学法检测62例GERD患者[其中反流性食管炎(RE组)患者30例,非糜烂性反流病(NERD组)患者32例]及10例正常对照食管下段上皮的紧密连接蛋白Occludin表达,并在光镜下观察切片并采集图像,采用图像分析系统对每例切片测量100个细胞间隙,进行统计学分析.结果:10例正常对照组Occludin呈正常连续的细胞膜表达,NERD组4例正常表达,28例不同程度异常表达,为细胞膜不完整表达、细胞质表达及表达缺失,30例RE均表达异常;RE组和NERD组Occludin阳性率与正常对照组比较,差异均有显著性(P<0.01),而RE组与NERD组间比较,无显著差异.RE组和NERD组平均细胞间隙与正常对照组比较,均有显著性差异(1.51±0.31μm,1.38±0.26μm vs0.77±0.12μm,均P<0.01),而RE组和NERD组间无显著差异.结论:胃食管反流病患者食管上皮Occludin数量减少、分布异常,导致细胞间隙增宽,影响黏膜上皮屏障的完整性,可能是胃食管反流病的发病机制之一. 展开更多
关键词 胃食管反流病 紧密连接蛋白 细胞间隙增宽 免疫组织化学 光镜测量
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美国发射星系间谍
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作者 小木 《太空探索》 2003年第6期9-9,共1页
关键词 美国 星系演化探测器 河外星系 紫外线分光镜测量 航天发射
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CHARACTER OF RETROREFLECTOR’S LIGHT FIELD’S ENERGY DISTRIBUTION AND ITS MEASURING TECHNOLOGY 被引量:2
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作者 杜颖 李真 张国雄 《Transactions of Tianjin University》 EI CAS 2000年第2期117-121,共页
The formation of the retroreflected light field is introduced in the paper and the components of the retroreflected light field are analyzed.Furthermore,a deep analysis of the factors affecting energy distribution of ... The formation of the retroreflected light field is introduced in the paper and the components of the retroreflected light field are analyzed.Furthermore,a deep analysis of the factors affecting energy distribution of the retroreflected light,such as design deviation,angle of incidence,was made.The simulation of the retroreflected light field was done.Recommendation is made in detail on both the energy distribution of the retroreflected light field at different working distances and the energy distribution of the retroreflected light field at a short distance when the diverging light comes.At last,two kinds of measuring instrument for the retroreflector are introduced,one is the long tunnel measuring instrument,the other is the minitype measuring instrument based on the character of the retroreflector when the diverging light comes. 展开更多
关键词 retroreflector retroreflected light field photometric measurement measuring instrument
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光学显微镜食管下段细胞间隙的测量对胃食管反流病诊断的意义 被引量:9
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作者 崔荣丽 周丽雅 +1 位作者 林三仁 薛艳 《中华内科杂志》 CAS CSCD 北大核心 2009年第3期208-212,共5页
目的通过光学显微镜(光镜)对胃食管反流病(GERD)患者食管下段细胞间隙的测量,探讨光镜下细胞间隙的变化对GERD的诊断意义。方法158例受试者行内镜检查,分为正常对照组(25例)、非糜烂性反流病(NERD)组(58例)和糜烂性食管炎... 目的通过光学显微镜(光镜)对胃食管反流病(GERD)患者食管下段细胞间隙的测量,探讨光镜下细胞间隙的变化对GERD的诊断意义。方法158例受试者行内镜检查,分为正常对照组(25例)、非糜烂性反流病(NERD)组(58例)和糜烂性食管炎(EE)组(75例),在齿状线以上2~3cm取活检行光镜检查,并随机选取43例(其中正常对照组4例,EE组28例,NERD组11例)在相同部位取活检同时进行电镜检查。光镜/电镜下观察切片并采集图像,采用图像分析系统对每例切片测量100个细胞间隙,并进行统计学分析。结果正常对照组、NERD组及EE组光镜下测量的平均细胞间隙(LMIS)分别为(0.61±0.10)μm、(1.12±0.61)μm和(1.30±0.19)μm,3组两两相比差异均有统计学意义(P〈0.05);EE组不同洛杉矶分级的各亚组间相比,细胞间隙增宽程度的差异无统计学意义(P〉0.05);NERD患者食管24hpH监测阳性者(40例)与阴性者(17例)LMIS的差异无统计学意义(P〉0.05);光镜下LMIS的截断(cut—off)值为0.85μm,如果以临床症状加内镜和食管24hpH监测综合作为诊断GERD的金标准,LMIS诊断GERD的敏感性为95.5%,特异性为100.0%;同时做光镜和电镜测量的受试者共43例,经相关性分析和一致性检验,光镜测量和电镜测量的细胞间隙宽度显著相关(r=0.737,P=0.000),且一致性好。结论GERD患者食管下段组织的细胞间隙可通过光镜进行定量测量,其结果与电镜测量结果的一致性好。光镜下细胞间隙增宽可以作为GERD诊断的敏感、特异且较为客观的指标之一。 展开更多
关键词 胃食管反流病 细胞间隙增宽 光镜测量 电镜
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3D Surface Morphology Measurement and Auto-focusing System
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作者 CHEN Qi ZANG Huai-pei 《Semiconductor Photonics and Technology》 CAS 2005年第3期208-211,共4页
When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur f... When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur for the measured object if there has small interference after clear focus. The auto-focusing system is introduced into the interference microscope, the system can obtain high definition interference image rapidly,and can improve the measuring velocity and measuring precision. The system is characterized by auto-focusing range of ±150 μm, auto-focusing precision of ±0.3 μm, auto-focusing time of 4~8 s. 展开更多
关键词 3D surface morphology Interference microscope AUTO-FOCUSING
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A displacement measurement system based on optical triangulation method 被引量:1
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作者 付献斌 刘彬 张玉存 《Optoelectronics Letters》 EI 2011年第5期380-383,共4页
A new displacement measurement system is described in this paper according to the basic principles of traditional lasertriangulation method. The range of the traditional measuring method is enlarged by measuring in se... A new displacement measurement system is described in this paper according to the basic principles of traditional lasertriangulation method. The range of the traditional measuring method is enlarged by measuring in sections. Three independent CCDs, which are distributed uniformly along the optical axis, are used to achieve subsection measurement. The planemirror is regarded as a virtual detector. When imaging beam is reflected by the plane mirror, the beam is imaged on theCC D. The designed system is equivalent to add a CCD. The feasibility of the displacement measurement system is verifiedby the experiment. 展开更多
关键词 TRIANGULATION
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Constraining photon mass by energy-dependent gravitational light bending
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作者 QIAN Lei 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2012年第3期523-526,共4页
In the standard model of particle physics, photons are massless particles with a particular dispersion relation. Tests of this claim at different scales are both interesting and important. Experiments in territory lab... In the standard model of particle physics, photons are massless particles with a particular dispersion relation. Tests of this claim at different scales are both interesting and important. Experiments in territory labs and several exterritorial tests have put some upper limits on photon mass, e.g., torsion balance experiment in the lab shows that photon mass should be smaller than 1.2 x 10-51g. In this work, this claim is tested at a cosmological scale by looking at strong gravitational lensing data available and an upper limit of 8.71 x 10-39g on photon mass is given. Observations of energy-dependent gravitational lensing with not yet available higher accuracy astrometry instruments may constrain photon mass better. 展开更多
关键词 ASTROMETRY SUN galaxy: nuclei
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