基于单细胞测序数据的拉克罗斯病毒感染神经系统细胞基因表达差异分析

目的分析受拉克罗斯病毒(LACV)感染的人脑类器官细胞不同亚群相关基因表达差异,揭示不同亚群之间的转录组特性。方法选择GEO数据库中编号为GSE131434的单细胞转录组数据,包含2个样本,1个为模拟感染COs的作为阴性对照组,1个为受LACV感染的COs。采用R语言“seurat”包对数据进行过滤、归一化、主成分分析、UMAP聚类分析,寻找COs亚群。通过“SingleR”包进行基因注释,“iTALK”包分析细胞通讯。通过“clusterProfile”包对差异基因进行富集分析,后构建PPI网络筛选关键基因。结果单细胞测序数据分析表明,受感染的COs能够分成7个细胞亚群,3种细胞类型,分别为浆细胞样树突状细胞、祖细胞、髓样树突状细胞,其中细胞通讯主要发生在祖细胞和浆细胞样树突状细胞之间,尤其是APOE-LDLR这对配体和受体中。样本的差异基因主要富集于翻译起始、mRNA和RNA分解过程、核糖体等过程中,与冠状病毒感染、帕金森等疾病的信号通路相关。进一步获得的4个关键基因为RPS27A、UBA52、RPLP0、RPL13A。结论拉克罗斯病毒感染对人脑类器官细胞的关键基因表达差异以及涉及APOE-LDLR的细胞通讯可能在感染过程中发挥关键作用,为未来的研究提供了重要线索。

Comparison of Influenza Outbreaks in the Republic of Kazakhstan and Russia Induced by 2009 Yearly New Variant of А(H1N1) Influenza Virus

The aim of the work is the comparison of the epidemiology of influenza and acute respiratory virus infections (ARVI) in the Republic of Kazakhstan with the corresponding influenza epidemic in Russia induced by influenza pandemic virus A/California/07/2009 in 2009.Data on influenza and ARVI from the Republic of Kazakhstan and Federal Center of influenza was collected and investigated over the course of several weeks from hospitalized patients with the same diagnosis among all population and in age groups on 16 territories of Kazakhstan and in 49 major cities of Russia.The epidemic in Kazakhstan resembled the Russian epidemic in terms of its abnormally early beginning,expression of monoaetiology,the spread of the epidemic into all territories and start of the epidemics among adult population.High percentage of hospitalized people and lethal outcome were registered in this epidemic.Similarity of epidemic process character in corresponding border-line territories of both countries was found out.