克隆测定法在卵巢恶性肿瘤中的应用

化疗是恶性肿瘤综合治疗主要措施之一,但并非所有化疗都能达到预期目的。相同组织类型,同样临床阶段的恶性肿瘤,对于不同的病人,即使用同一抗癌药物,同样治疗方案,其疗效常不一致。因为癌组织中癌细胞的异质性及不同肿瘤生物学特性的差异可影响肿瘤化疗敏感性,所以目前尚未发现某种抗癌药对所有的肿瘤都有效。据统计,凭临床经验选择药物进行肿瘤化疗的总有效率为14％,而大部分肿瘤病人化疗不但无效,而且化疗药物还可产生某些副作用及毒性作用,甚至可产生促癌作用。卵巢恶性肿瘤也不例外,化疗为主要手段之一,也具有上述不足之处。因此,近年许多学者致力于探讨测定个体肿瘤化疗敏感性的方法,目的是提高化疗疗效。

生长及克隆测定法对肿瘤活存细胞的检测

目的比较ＣｏｕｒｔｅｎａｙＭｉｌｓ克隆测定法和生长测定法对放射处理后活存肿瘤细胞的检测效果。方法选用人类乳腺癌ＭＣＦ７及结肠癌Ｃｏｌｏ２０５细胞株，在接受２Ｇｙ、４Ｇｙ、８Ｇｙ放射处理后，用以上两种方法检测活存的肿瘤细胞数。结果在克隆测定组，被检测的活存细胞均具有克隆能力；Ｃｏｌｏ２０５悬浮生长和贴壁生长细胞对放射线的敏感性没有差别。在生长测定组，“相对活存指数”于培养后第６～９天为最小，其平均值接近于克隆法。结论生长测定可以为细胞增殖的早期活动状态提供丰富的信息，而Ｃｏｕｒｔｅｎａｙ克隆测定则确切地反映了具有克隆能力的活存细胞数，并且适用于所有生长形式的细胞。

克隆酶供体免疫测定法在环孢菌素A浓度监测中的应用

目的 评价用自动生化分析仪测定全血环孢菌素A(cyclosporineA ,CsA)浓度的克隆酶供体免疫测定 (CEDIA)方法。方法 分析测定CEDIA方法的精密度、灵敏度、线性、准确性 (回收实验 )以及与荧光偏振免疫分析法 (FPIA/TDx)和酶扩大免疫测定法 (EMIT)之间的相关性。结果 CEDIA测定的批内CV分别为 1.8% (4 5 5 .7μg/L)、2 .2 9% (2 70 .5 μg/L)和 7.0 % (73.8μg/L) ;最低检出限为 7.0 μg/L ;测定线性范围可达 180 0 μg/L ;平均回收率为 99.4 % ;CEDIA法与FPIA/TDx和EMIT法进行对比 ,相关系数分别为 0 .931和 0 .94 5。结论 CEDIA方法测定CsA血液浓度具有操作简便、标本不需繁琐的前处理、精密度好和较宽的分析范围等特点 。

克隆酶供体免疫测定法检测25-羟基维生素D试剂盒的检测意义与性能评价

目的:介绍25-羟基维生素D的临床背景,并对复星长征公司研制生产的25-羟基维生素D测定试剂盒(克隆酶供体免疫测定法)进行初步方法学评价,为临床应用提供依据。方法:依据《临床化学体外诊断试剂(盒)通用技术要求》对25-羟基维生素D测定试剂盒(克隆酶供体免疫测定法)进行分析性能评估分析与验证,并评价其与其他厂家试剂的相关性。结果:试剂启用后可稳定2周;与英国Immunodiagnostic Systems Limited(IDS Ltd)公司生产的试剂测试结果相关性良好,其相关系数为0.9680。结论:复星长征公司研制生产的用克隆酶供体免疫测定法检测25-羟基维生素D试剂盒各项分析性能完全能够达到指标要求,其检测结果可为临床诊断提供科学依据。

M.M.Y-1型棉纤维马克隆测定仪省级设计定型会在山东省泰安市召开

M.M.Y—1型棉纤维马克隆测定仪,是山东省光学仪器厂根据国际标准 ISO2403—1972“棉纤维—马克隆测定法”中规定的有关技术指标,并在消化吸收美国思彬莱公司生产的 portar175气流仪基础上研制的。设计定型会于88年12月28日在泰安市召开.山东省国防工办、山东省纺织纤维检验所、泰安市棉麻公司,泰安棉纺针织厂及泰安纤检所等单位派员参加了会议。会上听取了山东光学仪器厂对该仪器的研制、标准化审查等有关报告和验证单位的验证试验报告吸使用单位意见。

Preparation and Application of Monoclonal Antibodies Specific for Salicylic Acid

Salicylic acid (SA) is widely distributed in many monocots and dicots and has many physiological effects. It can induce heat production in the thermogenic inflorescences of Arum lily([1]), block the biosynthesis of ethylene, and more attractively, it seems to be an important natural signal molecule in the induction of systemic acquired resistance (SAR) in tobacco, cucumber and other plants([2,3]). Studies in recent years showed that SA was also intimately related to the resistance of plants to aboitic stress, for example, SA increased chilling resistance of maize seedlings. Hence, SA has been accepted as a kind of new plant hormones. Up to date, the quantification of SA usually has been performed by HPLC[4,5], which often needs a large quantity of sample and a verbose pretreatment. Compared to HPLC, immunoassays, including radio-immunoassays (RIA) and enzyme-immunoassays (EIA), are easy to perform and have been widely used in the quantification of other plant hormones, such as IAA([6]), ABA([7,8]), GAs([9]), cytokinins et al([10]), and jasmonic acid (JA)([11]), and other low-molecular-weight, none-immunogenic compounds in plants([12]). Till now, only an indirect enzyme-linked immunosorbent assay (ELISA) for SA based on polyclonal antibodies (PAbs) has been developed by our group([13]), although Bennett et al([14]) had prepared SA PAbs using 4-aminosalicylic acid linked to KLH as immunogen in goat. However, the sensitivity of the ELISA we established formerly was relatively low, and also relatively larger quantity of sample is needed than other ELISAs for plant hormones. In this paper, an ELISA for SA based on monoclonal antibody raised against SA-NH-CH2-NH-KLH was introduced, and the fluctuation of SA content in cucumber leaves after inoculated with Pseudomonas syringae pv. syringae was determined.

丙型肝炎患者白细胞介素-2受体和肝功能损伤的相关研究

目的 探讨血清可溶性白细胞介素 - 2受体 (SIL- 2 R)、T淋巴细胞亚群等在丙型肝炎病毒感染中的作用。方法 应用双抗体夹心 EL ISA法和单克隆抗体测定法对 5 8例丙型肝炎患者进行了血清 SIL - 2 R和 T淋巴细胞亚群等进行相关研究 ,并以正常人 35名作对照。结果 丙型肝炎患者血清 SIL- 2 R水平显著地高于正常人组(P<0 .0 0 1) ,尤以肝硬变组明显。SIL - 2 R水平与 T细胞亚群中 CD8细胞比例 ,与 AL T密切相关。结论 检测丙型肝炎患者血清中 SIL- 2 R、T淋巴细胞亚群等水平可做为丙肝患者病情变化、预后判断及临床用药的监测指标。

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC-bovine serum albumin （BSA） conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA （icELISA） was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration （IC50） and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an ICs0 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross- reactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.