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垂体泌乳素腺瘤患者的激素分泌谱、克隆状态及临床病理学分析 被引量:3
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作者 马文斌 池田秀敏 吉本高志 《中华医学杂志》 CAS CSCD 北大核心 2005年第20期1382-1387,共6页
目的探讨影响垂体泌乳素瘤生物学行为的因素。方法123例垂体泌乳素腺瘤患者,其中男40例,女83例。将123例患者的肿瘤组织镜下标本经10%福尔马林或70%乙醇固定,进行常规病理及免疫组化分析。测定123例患者肿瘤切除前后的血清泌乳素水平。... 目的探讨影响垂体泌乳素瘤生物学行为的因素。方法123例垂体泌乳素腺瘤患者,其中男40例,女83例。将123例患者的肿瘤组织镜下标本经10%福尔马林或70%乙醇固定,进行常规病理及免疫组化分析。测定123例患者肿瘤切除前后的血清泌乳素水平。对26例女性患者肿瘤组织标本提取的DNA进行了HUMARA分析,确定肿瘤的克隆状态。结果123例患者中单激素分泌性61例(50%),多激素分泌性62例(50%)。患者年龄大小与血清泌乳素水平呈正相关(ρ=0.337,P<0.01),患者年龄与肿瘤体积呈正相关(ρ=0.378,P<0.01),肿瘤体积与血清泌乳素水平呈正相关(ρ=0.670,P<0.01)。多元回归分析提示,只有肿瘤体积与血清泌乳素水平存在明显相关。Mann-WhitneyU检验提示,泌乳素水平越高肿瘤体积越大,患者年龄越大,肿瘤侵袭海绵窦越明显。男性患者血清泌乳素水平明显高于女性患者,同时肿瘤体积明显较大。11例患者多激素分泌性垂体泌乳素腺瘤为单克隆起源。结论垂体泌乳素腺瘤患者除了分泌泌乳素外,还可以分泌多种垂体激素,而且绝大多数多激素分泌性垂体腺瘤的起源是单克隆性的。 展开更多
关键词 垂体泌乳素腺瘤 激素分泌谱 克隆状态 病理学 免疫组织化学
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基于动态马尔可夫模型的智能网页推荐 被引量:1
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作者 周晟 俞建家 《计算机工程与应用》 CSCD 北大核心 2006年第A01期84-87,共4页
马尔可夫模型被广泛应用于智能网页推荐中,研究表明高阶马尔可夫模型比低阶模型具有更高的推荐精度,但高阶模型存在着状态空间复杂度高的问题。提出了一种新颖的基于动态马尔可夫模型网页推荐方法,动态马尔可夫模型采用基于聚类的状态... 马尔可夫模型被广泛应用于智能网页推荐中,研究表明高阶马尔可夫模型比低阶模型具有更高的推荐精度,但高阶模型存在着状态空间复杂度高的问题。提出了一种新颖的基于动态马尔可夫模型网页推荐方法,动态马尔可夫模型采用基于聚类的状态克隆方法提高模型的精确度,同时通过设置精确度域值可以有效地控制模型的状态空间复杂度。 展开更多
关键词 WEB使用挖掘 动态马尔可夫模型 状态克隆 个性化推荐
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Scheme for Implementation of Optimal Cloning of Arbitrary Single Particle Atomic State into Two Photonic States
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作者 SONG Wei QIN Tao 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第6期1515-1518,共4页
We present a feasible scheme to implement the 1→2 optimal cloning of arbitrary single particle atomic state into two photonic states, which is important for applications in long distance quantum communication. Our sc... We present a feasible scheme to implement the 1→2 optimal cloning of arbitrary single particle atomic state into two photonic states, which is important for applications in long distance quantum communication. Our scheme also realizes the tele-NOT gate of one atom to the distant atom trapped in another cavity. The scheme is based on the adiabatic passage and the polarization measurement. It is robust against a number of practical noises such as the violation of the Lamb Dicke condition, spontaneous emission, and detection inefficiency. 展开更多
关键词 optimal cloning atomic state photonic state
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Implementing a Universal Quantum Cloning Machine via Adiabatic Evolution in Ion-Trap System
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作者 YANG Rong-Can LI Hong-Cai LIN Xiu HUANG Zhi-Ping XIE Hong 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第1期80-82,共3页
A scheme for the realization of a universal quantum cloning machine is proposed in this paper. The present protocol does not need the vibrational mode to act as the memory and it is robust against small changes of exp... A scheme for the realization of a universal quantum cloning machine is proposed in this paper. The present protocol does not need the vibrational mode to act as the memory and it is robust against small changes of experimental parameters due to adiabatic passages. Furthermore, the scheme may be realized based on current technology. 展开更多
关键词 universal quantum cloning adiabatic evolution dark state trapped ions
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Cloning and construction of sense and antisense eukaryotic expression vector of human Pin1
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作者 Wenhua Xiong Anmin Chen Fengjing Guo Tao Huang 《The Chinese-German Journal of Clinical Oncology》 CAS 2006年第5期358-361,共4页
Objective: To clone and construct eukaryotic expressing vectors of sense and antisense human Pin1 (hPinl) genes. Methods: Total RNA was extracted from MG-63 cells, then the hPinl cDNA was amplified by RT-PCR. The ... Objective: To clone and construct eukaryotic expressing vectors of sense and antisense human Pin1 (hPinl) genes. Methods: Total RNA was extracted from MG-63 cells, then the hPinl cDNA was amplified by RT-PCR. The same time the sense and antisense hPinl genes were formed by binding BamH Ⅰ and Hind Ⅲ in cis and trans-directions. At the end they were cloned into the eukaryotic expressing vector pIRES2-EGFP in cis and trans directions using DNA recombinant technology. The recombinant vectors were further identified by digestion of BamHⅠ and Hind Ⅲ. Results: The results of sequencing showed that the orientation of the ligations and the reading frame were correct. After digested by BamH Ⅰ and Hind Ⅲ, two fragments exhibiting 5.3 kb and 0.99 kb were formed in sense and antisense eukaryotic expressing vectors. Electrophoretic results were completely coincident with theoretical calculation. Conclusion: Human Pin1 sense and antisense genes were successfully cloned and eukaryotic expressing vectors were successfully constructed. 展开更多
关键词 PIN1 ISOMERASE antisense gene eukaryotic expressing vector
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Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum 被引量:3
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作者 Jia-ying ZHU Qi FANG +1 位作者 Gong-yin YE Cui HU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第2期93-102,共10页
Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially... Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoid-induced host alterations associated with innate immune responses, detoxification, and energy metabolism. 展开更多
关键词 PROTEOMICS PLASMA PARASITISM Gene cloning Pteromalus puparum Pieris rapae
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Implementing ancilla-free phase covariant quantum cloning with atoms trapped in cavities 被引量:1
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作者 YE Liu XIONG Wei +1 位作者 LI AiXia GUO GuangCan 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2011年第2期262-267,共6页
We propose a scheme to implement ancilla-free 1 to 2 optimal phase covariant quantum cloning with atoms trapped in cavities.In the scheme the W-class state of three atoms,which are individually trapped in three spatia... We propose a scheme to implement ancilla-free 1 to 2 optimal phase covariant quantum cloning with atoms trapped in cavities.In the scheme the W-class state of three atoms,which are individually trapped in three spatially separated cavities,is deterministically generated.Then by the use of this W-class state and detection of the atomic state,an optimal ancilla-free 1 to 2 phase-covariant quantum cloning between two spatially separated trapped atoms can be realized.The scheme is robust for atomic spontaneous decay,cavity decay and photon leakage out of the fiber.In principle,an M+1-atom W-class state can be generated by using such an approach.Thus this scheme also can be generalized to implement 1 to M ancilla-free phase-covariant quantum cloning. 展开更多
关键词 ancilla-free 1 to 2 OPTIMAL phase covariant quantum cloning atoms trapped in cavities
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Existence and construction of simultaneous cloning machines for mixed states
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作者 GUO ZhiHua CAO HuaiXin QU ShiXian 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2015年第4期11-15,共5页
It is a well-known fact that the no-cloning theorem forbids the creation of identical copies of an arbitrary unknown quantum state. In other words, there does not exist a quantum cloning machine that can clone all qua... It is a well-known fact that the no-cloning theorem forbids the creation of identical copies of an arbitrary unknown quantum state. In other words, there does not exist a quantum cloning machine that can clone all quantum states. However, it is possible to clone given quantum states under certain conditions, for instance, k distinct pure states |ψ1〉, |ψ2),....,|ψk) can be cloned simultaneously if and only if they are orthogonal. This paper discusses the existence and construction of simultaneous cloning machines for mixed states. It is proved that k distinct mixed states Pl,P2 Pk of the n-dimensional quantum system Cn can be cloned simultaneously, that is, there exists a quantum channel Ф on .Mn ⊙ Mn and a state ∑ in Mn, such that Ф(ρi ⊙∑) = ρi ⊙ ρi for all i, if and only if ρiρj = 0 (i ≠j). Also, the constructing procedure of the desired simultaneous cloning machine is given. 展开更多
关键词 simultaneous cloning machine quantum channel mixed state
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