Objective： To study the expression of eukaryotic initiation factor-4E （eIF-4E） and p53 in squamous cell carcinomas （SCC） and to explore their relationship and clinical significance. Methods： The expression of el...Objective： To study the expression of eukaryotic initiation factor-4E （eIF-4E） and p53 in squamous cell carcinomas （SCC） and to explore their relationship and clinical significance. Methods： The expression of elF-4E and p53 in 32 cases of SCC was detected by immunohistochemical SABC method. Results： The positive rate of elF-4E and p53 expression was 93.8% and 56.3% in SCC, and the levels of eIF-4E and p53 were significantly higher in SCC than those in the normal skin tissue （P 〈 0.05）. Conclusion： Both elF-4E and p53 were useful markers in SCC, but the specialty and sensitivity of the eiF- 4E protein was high in SCC.展开更多
Objective： To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods： Detecting proteins of p130Cas, PTEN and PTEN mRNA ...Objective： To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods： Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results： The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa （P 〈 0.05）. Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group （P 〈 0.05）. The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group （P 〈 0.001）. Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion： The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it.展开更多
文摘Objective： To study the expression of eukaryotic initiation factor-4E （eIF-4E） and p53 in squamous cell carcinomas （SCC） and to explore their relationship and clinical significance. Methods： The expression of elF-4E and p53 in 32 cases of SCC was detected by immunohistochemical SABC method. Results： The positive rate of elF-4E and p53 expression was 93.8% and 56.3% in SCC, and the levels of eIF-4E and p53 were significantly higher in SCC than those in the normal skin tissue （P 〈 0.05）. Conclusion： Both elF-4E and p53 were useful markers in SCC, but the specialty and sensitivity of the eiF- 4E protein was high in SCC.
基金Supported by a grant from Sanitary Science and Technological Development Foundation of Shandong Province (No. 2003HW015)
文摘Objective： To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods： Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results： The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa （P 〈 0.05）. Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group （P 〈 0.05）. The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group （P 〈 0.001）. Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion： The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it.
