Objective: To observe human to mouse one way mixed lymphocyte(MLC); And to set up the xeno-grats verse host disease Xeno-graft host disease(XGVHD) model,probing its immunologic mechamism.Methods: Mouse splenic lympho...Objective: To observe human to mouse one way mixed lymphocyte(MLC); And to set up the xeno-grats verse host disease Xeno-graft host disease(XGVHD) model,probing its immunologic mechamism.Methods: Mouse splenic lymphocyte were collected in asepsis and treated by mitomycin as activating cell. Human Peripheral blood lymphocytes (hPBL)were separated and gathered as reacting cell; Mouse splenic lymphocyte and hPBL were mixed to incubate for a week. Destroying recipient (mouse) immune system by total body irradiation (TBI) and intraperitoneal injecting CTX、MTX; Separating and collecting hPBL; Injecting hPBL to mouse by caudal vein. Results; ①HPBL in the experiment groups(mixed mouse lymphocyte) proliferated obviously, the amount of 3H-TdR in corporation increased evidently(P<0.05); The mean percentage of CD 4、CD 8、IgG 、IgM positive cells rose markedly. ②Experiment groups,the hPBL were found in the spleen and kidney tissue, fas protein expressing, we occasionally discovered and apoptosis cells.Conclusion: The human to mouse one-way MLC has obvious lymphocyte proliferation. By these means,we succeed in inducing XGVHD and setting up a XGVHD model.展开更多
The cholinergic anti-inflammatory pathway （CAP） is a neurophysiological mechanism that regulates the immune system. The CAP inhibits inflammation by suppressing cytokine synthesis via release of acetylcholine in org...The cholinergic anti-inflammatory pathway （CAP） is a neurophysiological mechanism that regulates the immune system. The CAP inhibits inflammation by suppressing cytokine synthesis via release of acetylcholine in organs of the reticuloendothelial system, including the lungs, spleen, liver, kidneys and gastrointestinal tract. Acetylcholine can interact with a 7 nicotinic acetylcholine receptors （ a 7 nAchR） expressed by macrophages and other cytokine producing cells, down-regulate pro-inflammatory cytokine synthesis and prevent tissue damage. Herein is a review of the neurophysiological mechanism in which the CAP regulates inflammatory response, as well as its potential interventional strategy for inflammatory diseases.展开更多
Takayasu's arteritis(TA),also known as the "pulseless disease," is a chronic vasculitis of the aorta and aortic branches.TA with Crohn's disease is rare and has not been documented in China before.In...Takayasu's arteritis(TA),also known as the "pulseless disease," is a chronic vasculitis of the aorta and aortic branches.TA with Crohn's disease is rare and has not been documented in China before.In this paper we report on a case of Takayasu's arteritis associated with concurrent Crohn's disease.A 17-year-old Chinese male developed upper limb sourness and a sensation of fatigue,and his upper limb pulses were absent.He was diagnosed with TA and underwent an axillary artery bypass with autologous great saphenous vein on the left subclavian artery.After the surgery,he regained the normal blood pressure.This patient also had years of diarrhea and developed an anal canal ulcer,and was diagnosed with inflammatory bowel disease and ulcerative colitis before.Five months after the TA surgery,he was hospitalized for severe stomachache and diarrhea and was finally diagnosed with Crohn's disease.The possible pathophysiological mechanisms responsible for concurrent existence of TA and Crohn's disease may be associated with immune disorders,especially autoimmunity.展开更多
文摘Objective: To observe human to mouse one way mixed lymphocyte(MLC); And to set up the xeno-grats verse host disease Xeno-graft host disease(XGVHD) model,probing its immunologic mechamism.Methods: Mouse splenic lymphocyte were collected in asepsis and treated by mitomycin as activating cell. Human Peripheral blood lymphocytes (hPBL)were separated and gathered as reacting cell; Mouse splenic lymphocyte and hPBL were mixed to incubate for a week. Destroying recipient (mouse) immune system by total body irradiation (TBI) and intraperitoneal injecting CTX、MTX; Separating and collecting hPBL; Injecting hPBL to mouse by caudal vein. Results; ①HPBL in the experiment groups(mixed mouse lymphocyte) proliferated obviously, the amount of 3H-TdR in corporation increased evidently(P<0.05); The mean percentage of CD 4、CD 8、IgG 、IgM positive cells rose markedly. ②Experiment groups,the hPBL were found in the spleen and kidney tissue, fas protein expressing, we occasionally discovered and apoptosis cells.Conclusion: The human to mouse one-way MLC has obvious lymphocyte proliferation. By these means,we succeed in inducing XGVHD and setting up a XGVHD model.
基金This study was supported by grants from the National Natural Science Foundation of China （No. 30672178, 30872683, 30800437） and the National Basic Research Program of China （No. 2005CB522602）.
文摘The cholinergic anti-inflammatory pathway （CAP） is a neurophysiological mechanism that regulates the immune system. The CAP inhibits inflammation by suppressing cytokine synthesis via release of acetylcholine in organs of the reticuloendothelial system, including the lungs, spleen, liver, kidneys and gastrointestinal tract. Acetylcholine can interact with a 7 nicotinic acetylcholine receptors （ a 7 nAchR） expressed by macrophages and other cytokine producing cells, down-regulate pro-inflammatory cytokine synthesis and prevent tissue damage. Herein is a review of the neurophysiological mechanism in which the CAP regulates inflammatory response, as well as its potential interventional strategy for inflammatory diseases.
文摘Takayasu's arteritis(TA),also known as the "pulseless disease," is a chronic vasculitis of the aorta and aortic branches.TA with Crohn's disease is rare and has not been documented in China before.In this paper we report on a case of Takayasu's arteritis associated with concurrent Crohn's disease.A 17-year-old Chinese male developed upper limb sourness and a sensation of fatigue,and his upper limb pulses were absent.He was diagnosed with TA and underwent an axillary artery bypass with autologous great saphenous vein on the left subclavian artery.After the surgery,he regained the normal blood pressure.This patient also had years of diarrhea and developed an anal canal ulcer,and was diagnosed with inflammatory bowel disease and ulcerative colitis before.Five months after the TA surgery,he was hospitalized for severe stomachache and diarrhea and was finally diagnosed with Crohn's disease.The possible pathophysiological mechanisms responsible for concurrent existence of TA and Crohn's disease may be associated with immune disorders,especially autoimmunity.
