口服枸杞多糖对实验性食管癌大鼠免疫功能影响的研究

枸杞多糖（lycium barbarum polysaccharides,LBP）作为枸杞子的主要活性成分已日益得到临床医生的高度重视。研究表明,LBP有增强机体的免疫功能和显著的抗肿瘤效应,它能从调动机体自身的抗病能力出发,在提高机体免疫力的同时杀伤肿瘤细胞[1];它能促进骨髓有核细胞数和粒/巨噬细胞集落数量的增加（CFU-GM）;

缺铁性贫血对仔猪免疫功能的不良影响

采用醋酸纤维薄膜电泳分析法和3H—TdR掺入法,研究了仔猪缺铁性贫血对免疫功能的影响。试验于补铁治疗前后检测了血常规3个指标、免疫学2个指标,结果显示:出生后不予补铁的仔猪,其血红蛋白(Hb.)、红细胞压积(PCV)、红细胞数(RBC)均显著降低,从而严重影响了仔猪的免疫功能,其中对细胞免疫功能的影响尤为显著(P<0.01),补铁治疗后血常规指标升高。外周血淋巴细胞3H—TdR掺入量显著提高(P<0.01),而血清丙球蛋白(γ—Ig)百分比的平均值略高于不治疗对照组,但差异无显著性。(P>0.05)。

胸腺肽α1在提高激素耐药型肾病综合征患儿细胞免疫功能中的作用

目的：分析胸腺肽α1在提高激素耐药型肾病综合征（SRNS）患儿细胞免疫功能中的作用.方法：选取从2013年4月-2014年3月来我院接受治疗的原发性SRNS患儿70例,随机分成实验组和对比组,每组各35例,对对比组患者行以泼尼松治疗为主的常规治疗,在此基础上对实验组行胸腺肽α1治疗.结果：相对于对比组,实验组CD4＋和CD4＋/CD8＋更高、CD8＋更低,差异均具有统计学意义（P〈0.05）.CD3＋比较,2组之间的差异不具有统计学意义（P〉0.05）.有效率比较,2组差异不具有统计学意义（P〉0.05）,复发率比较,实验组更低,差异有统计学意义（P〈0.05）.结论：胸腺肽α1具有提高激素耐药型肾病综合征患儿细胞免疫功能作用,减少肾病的复发,达到良好的临床效果.

紫菜多糖对免疫低功小鼠脾细胞功能的影响

目的探讨紫菜多糖对免疫低功小鼠脾细胞功能的影响。方法小鼠腹腔注射不同剂量(0.025,0.050和0.150g·kg-1)的紫菜多糖,每日1次,连续7d,并在给予紫菜多糖的第1天和第2天同时注射环磷酰胺造成免疫功能低下模型,第8天检测小鼠脾淋巴细胞的增殖及TNF的生物学活性。结果0.150g·kg-1紫菜多糖组小鼠的淋巴细胞增殖能力及TNF的细胞毒活性均高于模型组。结论适宜浓度的紫菜多糖能够促进淋巴细胞增殖,提高TNF的产生。

经济责任审计实务探析——基于审计“免疫系统”功能和审计视角的思考

审计是经济社会发展过程中的重要环节,审计在组织建构和发展过程中具有“免疫系统”功能.事业单位的审计具有纠错、预警、震慑和建设的作用.从审计具有“免疫系统”功能的角度看,审计需要向管理、控制和风险把控转变.

甲功五项化学发光免疫分析的基质效应对临床测值的影响

目的探究甲功五项化学发光免疫分析的基质效应对临床测值的影响。方法探究T3、T4、FT3、FT4、TSH项目的牛血清、马血清、山羊血清、水解明胶、BSA、PBS、生理盐水、正常人血清、甲减人血清的基质效应对临床测试的影响。结果用不同基质对同一份高值血清进行倍比稀释,T3项目中的牛血清、水解明胶、BSA基质效应较为明显,T4项目中的牛血清、马血清、水解明胶、BSA、PBS、生理盐水基质效应明显,FT3项目中的牛血清、水解明胶、BAS、PBS、生理盐水基质效应明显,FT4项目中的牛血清、马血清、水解明胶、BSA、PBS、生理盐水基质效应明显。结论使用甲减人血清对T3、T4、FT3、FT4、TSH项目高值血清进行稀释,甲亢人血清在促甲状腺激素稀释中取得了良好的稀释效果。

匹多莫德治疗小儿反复呼吸系统感染的疗效观察

目的:探讨匹多莫德治疗小儿反复呼吸系统感染的临床效果及对免疫功能的影响。方法:选取130例反复呼吸系统感染患儿,随机分为对照组和观察组各65例,对照组给予常规综合治疗,观察组在对照组的基础上给予匹多莫德颗粒口服治疗。比较两组患儿的治疗效果及两组患儿治疗前、治疗后3个月时Ig A、Ig G、Ig M水平变化。结果:观察组总有效率明显高于对照组(P<0.05),Ig A、Ig G、Ig M水平,对照组治疗前后比较无明显变化(P>0.05),观察组治疗后较治疗前明显升高(P<0.05)。结论:匹多莫德治疗小儿反复呼吸系统感染疗效显著,可改善其免疫功能,减少感染次数,减轻症状,缩短病程,安全可靠,值得推广应用。

Modulation of Lianbizi Injection (Andrographolide ) on Some Immune Functions

Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) culture supernatants dealt with by different concentrations of LianBiZhi (LBZ) injection, the effective component of which is andrographolide, were detected by biological activity test or ELISA in vitro. The effects of LBZ injection on macrophage phagocytotic function and natural killer cells cytotoxicity were examined by means of macrophage to phagocytize cock erythrocyte and measurement of lactate dehydrogenase (LDH) activity released from the damaged cells, respectively.Results: The LBZ injection could not only enhance the phagocytosis activity of peritoneal macrophage from guinea pig to phagocytosis cock erythrocytes, but also augment the cytotoxicity mediated by natural killer cells from PBMCs.Conclusion: Andrographolide is an immunostimulant agent which can modulate both antigen specific and nonspecific immune function by means of its natural killer cells, macrophage and cytokines.

Effects of Compound Phellinus igniarius(L.ex Fr.)Quel. Oral Liquid's Polysaccharide on the Immunologic Function of Mice

In this paper, the effects of compound P. igniarius oral liquid’s crude polysaccharide on the immunologic function of mice were studied from four aspects, namely, carbon clearance test of mice, macrophage phagocytosis of chicken red blood cel s in the enterocoelia of mice （semi-in vivo method）, the ratio of organ to body weight, natural kil er （NK） cel activity in mice （the determination of lactate de-hydrogenase assay）. The results showed that high-dosage group（40 mL/kg） of com-pound P. igniarius oral liquid can obviously enhance the ability of carbon clearance of mice; middle-（20 mL/kg） and high-dosage groups can significantly enhance the phagocytic rate and phagocytic index of chicken erythrocyte of mouse macrophage;low-（10 mL/kg）, middle- and high-dosage groups can significantly enhance NK cel activity of mice. These showed that compound P. igniarius oral liquid can enhance mononuclear-macrophage and NK cel activity. In conclusion, compound P. igniarius oral liquid’s polysaccharide can enhance immunologic function and significantly im-prove the specific and nonspecific immunologic function.

IMMUNE FUNCTION OF IMMATURE RAINBOW TROUT ( Oncorhynchus mykiss ) RELATED WITH PHOTOPERIOD

Radioimmunoassay,enzyme linked immunospot assay and enzyme immunoassay were used for the determinations of plasma steroid hormone's level,antibody producing cell's counting and IgM level in this study.The decreased number of antibody producing cells and low IgM levels were observed in sexual immature rainbow trout during the spawning season.These fish were reared under almost constant water temperature and natural photoperiod.Moreover,low IgM level was also observed in immature rainbow trout,which were reared under short photoperiod,and IgM level was not changed by treatment of testosterone.The results suggest that photoperoid may cause the changes in immune competence.It is possible that circadian rhythm accompanied with photoperiod may influence physiological function of fish,so that immune competence is changed.

A Review on the Biological Characteristics and Secretory Functions of Adipose-derived Mesenchymal Stem Cells

Adipose-derived mesenchymal stem cells （ADSCs） can be largely and easily obtained from a wide range of sources. Moreover, they have self-renewal ability, multi-differentiation potential, and an important role in immune regulation. They can secrete a variety of cytokines to regulate the in vivo micro-environment. Therefore, ADSCs are the ideal seed ceils for stem ceils application. This paper reviews the location, isolation, surface markers, proliferation, differentiation and other biological characteristics of ADSCs, as well as their secretory function and relative researches. ADSCs are expected to become excellent seed cells for cell therapy and tissue engineering through in-depth studies.

Effect of Cistanoside Compounds on Oxidative Stress and Immunity

Cistanoside compounds were studied as the scavengers of hydroxyl and superoxide anion free radicals with spin trapping ESR method in vitro. Low-temperature ESR technique, experimental technique of immunotoxicology and biochemical method were used to detect the level of reactive oxygen radicals in kidney tissue of rats and SOD level and GSH-Px activity in rat serum. The results indicated that cistanoside compounds could inhibit reactive oxygen free radicals in vitro and prevent and repair the free radical damages for diabetic nephropathy. The experimental data of 揷arbon-particle detection in mouse serum?showed that cistanoside compounds could improve the phagocytotis index of macrophages (Mj) in mice blood and increase the weights of immune organs of mice.

Effects of Echinacea Compound on the Immune Function of Weaned Piglets

[Objective] This study aimed to investigate effects of Echinacea compound on the immune function of weaned piglets. [Method] Eighty Duroc x Landrace x Yorkshire crossbred piglets were randomly divided into four groups： control group （drug-free group）, 1.5% close group, 1.0% dose group and 0.5% dose group. Blood samples of piglets were collected at 20, 35, 50, 60, 70 and 80 days old, respective- ly, to determine neutrophil leukocyte percentage in peripheral blood, lymphocyte trapsformation rate and the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder. [Result] Applying a certain dose of Echinacea compound could significantly increase neutrophil leukocyte per- centage in peripheral blood and lymphocyte transformation rate （P〈0.05）, and ex- tremely significantly improve the levels of antibodies against classical swine fever, porcine reproductive and respiratory syndrome disorder （P〈0.01）. [Conclusion] Echi- nacea compound has played a certain role in promoting nonspecific and specific im- mune function of piglets.

夏日话午睡

夏天来临,日长夜短,人容易感到困倦,尤其是午后,睡意更袭人。 人在睡眠峰期不睡眠,不仅对健康无益,而且容易导致车祸、猝死等。日本警视厅“科学警察研究所”调查发现。

秋凉谨防老年人肺炎

秋季,尤其是金秋时节,老年人肺炎的发病率和死亡率骤然增高。 中医认为,燥是秋之主气,五脏属肺,肺为“娇脏”,极易伤其燥,导致肺虚,引发肺炎。从生理上看,老年人呼吸道老化,支气管上皮萎缩,杯状细胞增生,纤毛运动减弱,清除功能下降,再加之胸腺退化,T细胞减少,免疫功能差,极易发生肺炎。另外,老年人往往患有“老慢支”等,抵抗力弱,适应性差,在天气由热转凉、昼夜温差悬殊时。

延年益寿的心疗处方

最近,美国研究人员对700名百岁老人进行了3年跟踪研究,揭开了他们的长寿秘密:性格开朗,很少发愁,基本不发火,一辈子心平气和。可见,长寿之道不分中外。"没心没肺"者最长寿今年84岁高龄的国医大师陆广莘满头乌发,从相貌上看也就50岁左右,

Effects of Collagen Peptide from Cyanea nozakii on Mouse Immune Function

[Objective ] The study aimed to provide a theoretical basis for the clinical application of collagen peptide from Cyanea nozakii. [ Method] After acute toxicity test on mice, collagen peptide from C. nozakii were given to mice by continuous intragastric administration for 30 d at the doses of 25, 50, 100 mg/kg, and then the phagocytosis of macrophage, delayed type hypersensitivity （DTH） and serum hemolysin level were determined. [ Result] Collagen peptide from C. nozakii was atoxic or low toxic, and the three immune indices of experimental groups were signifi- canUy higher than those of the control group （treated with same volume of normal saline） at 0.05 or 0.01 level. E Conclusion] Collagen peptide from C. nozakii has a certain immunopotentiation.

Changes in count and function of splenic lymphocytes from patients with portal hypertension

AIM: To investigate changes in numbers and proliferative function of splenic lymphocytes in patients with hypersplenism due to portal hypertension (PH), to provide evidence for further study of immune status of the spleen during PH. METHODS: Twelve spleens from patients with hypersplenism due to PH served as the PH group, and four spleens from cases of traumatic spleen rupture were regarded as the control group. After weighing the spleen, lymphocytes were separated and counted using a cell counting plate to calculate the lymphocyte count per gram of spleen tissue (relative quantity) and total lymphocyte count in whole spleen (absolute quantity). The immunohistochemical SP method was used to observe the density and distribution of lymphocytes in the spleen. The MTT method was used to observe changes in lymphocyte proliferative function. RESULTS: As compared to the control group, the splenic lymphocytes in the PH group showed that: (1) There was no difference in distribution but a significant decreasein density; (2) the number of lymphocytes per gram of spleen (relative quantity) decreased significantly (0.822 ± 0.157) × 108 vs (1.174 ± 0.254) × 108, P < 0.01]; (3) with the significant increase in the weight of the PH spleen (832.6 ± 278.2 g vs 211.7 ± 85.6 g, P < 0.01), the total quantity of lymphocytes (absolute quantity) increased significantly (0.685 ± 0.072) × 1011 vs (0.366 ± 0.057) × 1011, P < 0.01]; and (4) the proliferative function of lymphocytes was enhanced: T lymphocytes, (0.022 ± 0.005 vs 0.015 ± 0.003, P < 0.05), and B lymphocytes (0.034 ± 0.006 vs 0.023 ± 0.001, P < 0.01). CONCLUSION: Although lymphocyte density in the spleen decreased in patients with PH, the total quantity of lymphocytes increased because spleen weight increased greatly, along with the proliferating function. With respect to changes in lymphocytes, PH spleens may still have immune function, although it may be disordered. However, complete evaluation of the immune function of the spleen in PH requires more research.

Effects of a Shuangling Fuzheng anticancer preparation on the proliferation of SGC-7901 cells and immune function in a cyclophosphamide-treated murine model

AIM: To study the inhibitory effects of a Shuangling Fuzheng anticancer preparation (SFAP) on the human gastric cancer cell line SGC-7901 in vitro as well as its immune-modulated effects in a cyclophosphamide-treated murine model. METHODS: MTT experiments and immunocytochemistry ABC experiments were performed for detecting the proliferation of SGC-7901 cells in vitro and protein expression of c-myc. The staphylococcal protein A (SPA) rosette test was utilized for measuring the ratio of T-lymphocyte subsets from peripheral blood in a cyclophosphamide-treated murine model. Enzyme- linked immunosorbant assay (ELISA) was performed for measuring the levels of serum sIL-2R in treated mice, while immunoturbidimetry was used for measuring the levels of immunoglobulins (Ig). RESULTS: SFAP (40-640 mg/L, 48 h) inhibited the proliferation of SGC-7901 cells, and a positive correlation was noted between inhibitory effects and dosage. At a dosage of 160-320 mg/L in cultured cells, the expression of c-myc was decreased. SFAP (50-200 mg/kg) increased the percentage of CD3+ and CD4+ T-lymphocytes, the ratio of CD4/CD8, and the contents of Ig such as IgM, IgG or IgA, but decreased the levels of serum sIL-2R in peripheral blood from cyclophosphamide-treated mice. CONCLUSION: SFAP can inhibit the proliferation of SGC-7901 cells via the c-myc gene. In addition, SFAP can modulat the cellular and humoral immunity in cyclophosphamide-induced immunosuppressed mice.

Hepatitis B virus DNA is more powerful than HBeAg in predicting peripheral T-lymphocyte subpopulations in chronic HBV-infected individuals with normal liver function tests

AIM:To investigate the peripheral T-lymphocyte subpopulation profile,and its correlations with hepatitis B virus(HBV) replication level in chronic HBV-infected(CHI) individuals with normal liver function tests(LFTs) . METHODS:Frequencies of T-lymphocyte subpopu-lations in peripheral blood were measured by flow cytometry in 216 CHI individuals. HBV markers were detected with ELISA. Serum HBV DNA load was assessed with quantitative real-time PCR. Information of age at HBV infection,and maternal HBV infection status was collected. ANOVA linear trend test and linear regression were used in statistical analysis. RESULTS:CHI individuals had significantly decreased relative frequencies of CD3+,CD4+ subpopulationsand CD4+/CD8+ ratio,and increased CD8+ subset percentage compared with uninfected individuals(all P < 0.001) . There was a significant linear relationship between the load of HBV DNA and the parameters of T-lymphocyte subpopulations(ANOVA linear trend test P < 0.01) . The parameters were also significantly worse among individuals whose mothers were known to be HBV carriers,and those having gained infection before the age of 8 years. In multiple regressions,after adjustment for age at HBV infection and status of maternal HBV infection,log copies of HBV DNA maintained its highly significant predictive coefficient on T-lymphocyte subpopulations,whereas the effect of HBeAg was not significant. CONCLUSION:HBV DNA correlates with modification in the relative T-lymphocyte subpopulation frequencies. High viral load is more powerful than HBeAg in predicting the impaired balance of T-cell subsets.