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不同检测方法对低水平乙型肝炎表面抗原的测定及其临床评估 被引量:4
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作者 费春荣 叶爱青 张钧 《浙江大学学报(医学版)》 CAS CSCD 北大核心 2011年第4期436-439,共4页
目的:探讨免疫发光、电化学免疫发光、ELISA三种方法对检测低水平乙型肝炎表面抗原的价值。方法:以免疫发光测试系统Architect i2000检测结果为参考,把70例<50 IU/ml的标本分成三个级别:<1 ng/ml、1~5 ng/ml、≥5 ng/ml三组,用电... 目的:探讨免疫发光、电化学免疫发光、ELISA三种方法对检测低水平乙型肝炎表面抗原的价值。方法:以免疫发光测试系统Architect i2000检测结果为参考,把70例<50 IU/ml的标本分成三个级别:<1 ng/ml、1~5 ng/ml、≥5 ng/ml三组,用电化学免疫发光MODULAR〈E〉170、ELISA检测,对三种方法测定的结果进行比较。结果:免疫发光Architect i2000与电化学免疫发光MODULAR〈E〉170比较,<1 ng/ml组符合率79.2%(P(0.05),1~5 ng/ml组符合率100%,≥5ng/ml组符合率100%。免疫发光Architect i2000与ELISA比较,<1 ng/ml组符合率0%,1~5 ng/ml组符合率45.5%(P<0.05),≥5 ng/ml组符合率100%。结论:免疫发光Architect i2000与电化学免疫发光MODULAR〈E〉170对低水平HBsAg的检测效能基本一致,结果相对可靠,常规ELISA法对≥5 ng/ml的HBsAg检测结果可靠,但对低水平HBsAg漏检率高,特别是<1 ng/ml HBsAg很难检出。 展开更多
关键词 肝炎表面抗原 乙型/分析 酶联免疫吸附测定/方法 乙型肝炎表面抗原 免疫发光测定 电化学免疫发光测定 ELISA
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Determination of theophylline concentration in serum by chemiluminescent immunoassay 被引量:2
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作者 周美霞 管茶英 +2 位作者 陈光 谢鑫友 吴盛海 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第12期1148-1152,共5页
Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and eval... Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline. 展开更多
关键词 THEOPHYLLINE Chemiluminescent immunoassay (CLIA) Fluorescence polarization immunoassay (FPIA)
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Fast Diagnosis of Gonorrhea With Enhanced Luminescence Enzyme Immunoassay
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作者 郑和义 曹经江 邵燕玲 《Chinese Journal of Sexually Transmitted Infections》 2002年第1期44-45,共2页
Objective:To evaluate the value of enhanced luminescence enzyme immunoassay in the diagnosis of Neisseria gonorrhea(NG) infection. Methods: Anti-catalase antibody for Neisseria gonorrheae combined with enhanced lumine... Objective:To evaluate the value of enhanced luminescence enzyme immunoassay in the diagnosis of Neisseria gonorrhea(NG) infection. Methods: Anti-catalase antibody for Neisseria gonorrheae combined with enhanced luminescence enzyme immunoassay were used to test for N. gonorrhea. Results: A minimum of 1×10^4/CFU of GC in genital tract secretions or urine could be detected with the technique of luminescence enzyme immunoassay. Conclusion : The enhanced luminescence enzyme immunoassay has the advantage of high sensitivity and specificity for diagnosing NG from genitourinary tract secretion and urine. 展开更多
关键词 Neisseria gonorrhoeae Enhanced luminescence Enzyme immunoassay
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