微波免疫组化SP法在长角血蜱体内伯氏疏螺旋体形态研究中的应用

目的 观察长角血蜱体内伯氏疏螺旋体的免疫形态特征。方法 1996年 5月 ,对北京西山采集的羊体表长角血蜱寄生蜱标本行微波免疫组化SP法检测和伯氏疏螺旋体形态观察。结果 光镜下 ,寄生蜱中肠内显示伯氏疏螺旋体菌体数量多且形态多样。螺旋体螺旋波纹呈不规则锯齿形 ,或呈纤细疏螺旋形。短形螺旋体有 3～ 10个疏螺旋 ,而长形螺旋体达 30个以上的疏螺旋。结论 微波抗原修复免疫组化SP法染色 ,蜱中肠标本中伯氏疏螺旋体显示强阳性 ,易于伯氏疏螺旋体的检出和形态观察。

Analysis of clinicopathological and immunohistochemical features of 15 cases with olfactory neuroblastoma

Objective:We studied the clinicopathological and immunohistochemical features of olfactory neuroblastoma(ONB).Methods:The clinic pathological data and immunohistochemical features of 15 cases with ONB were analyzed retrospectively,and the related literatures were reviewed.Results:The tumors were all located in the nasal cavity in 15 cases.According to Kadish's staging system,7 cases were in stage A,5 cases in stage B,and 3 cases in stage C.The morphological features showed small round or ovoid tumor cells divided into pieces or trabeculae by connective tissues which were rich in capillaries.The tumor cells had round or oval nuclei and fine chromatins and lack of cytoplasma.Flexner rosette and Homer-Wright rosette were found in some cases.Acidophilic fibrins were composed of cytoplasmic projection among tumor cells.All cases were positive for NSE,Syn,CgA,1 case was positive for Vimentin,2 cases were positive for S-100,while CKpan,LCA and HMB45 were all negative.Conclusion:ONB is a type of very rare malignant tumors,which could be diagnosed by pathology,and immunohistochemistry is helpful in the diagnosis and differential diagnosis.