目的探讨冷驯化不同时期中缅树鼩脂肪细胞形态变化,为冷驯化是否可以诱导白色脂肪组织中米色脂肪细胞(beige adipocytes)的产生提供形态学依据。方法通过苏木素-伊红染色切片、免疫组织化学染色切片、免疫荧光染色切片进行对比。结果随...目的探讨冷驯化不同时期中缅树鼩脂肪细胞形态变化,为冷驯化是否可以诱导白色脂肪组织中米色脂肪细胞(beige adipocytes)的产生提供形态学依据。方法通过苏木素-伊红染色切片、免疫组织化学染色切片、免疫荧光染色切片进行对比。结果随着冷驯化时间延长,白色脂肪细胞和褐色脂肪细胞形态都明显变小,且褐色脂肪组织中解偶联蛋白1(uncoupling protein 1,UCP1)表达增加,同时腹部皮下白色脂肪组织中可能已发生褐变,产生一些具有UCP1表达的浅褐色脂肪细胞,即米色脂肪细胞。结论不同染色切片不仅能够清晰观察到脂肪细胞的形态改变,还能从抗体阳性颜色中观察出UCP1表达变化,为中缅树鼩脂肪组织研究提供基础性材料。展开更多
AIM:To investigate morphological changes of intestinal smooth muscle contractile fibres in small bowel atresia patients.METHODS:Resected small bowel specimens from small bowel atresia patients(n = 12) were divided int...AIM:To investigate morphological changes of intestinal smooth muscle contractile fibres in small bowel atresia patients.METHODS:Resected small bowel specimens from small bowel atresia patients(n = 12) were divided into three sections(proximal,atretic and distal).Standard histology hematoxylin-eosin staining and enzyme immunohistochemistry was performed to visualize smooth muscle contractile markers-smooth muscle actin(SMA) and desmin using conventional paraffin sections of the proximal and distal bowel.Small bowel from agematched patients(n = 2) undergoing Meckel's diverticulum resection served as controls.RESULTS:The smooth muscle coat in the proximal bowel of small bowel atresia patients was thickened compared with control tissue,but the distal bowel was unchanged.Expression of smooth muscle contractile fibres SMA and desmin within the proximal bowel was slightly reduced compared with the distal bowel and control tissue.There were no major differences in the architecture of the smooth muscle within the proximal bowel and the distal bowel.The proximal and distal bowel in small bowel atresia patients revealed only minimal differences regarding smooth muscle morphology and the presence of smooth muscle contractile filament markers.CONCLUSION:Changes in smooth muscle contractile filaments do not appear to play a major role in postoperative motility disorders in small bowel atresia.展开更多
文摘目的探讨冷驯化不同时期中缅树鼩脂肪细胞形态变化,为冷驯化是否可以诱导白色脂肪组织中米色脂肪细胞(beige adipocytes)的产生提供形态学依据。方法通过苏木素-伊红染色切片、免疫组织化学染色切片、免疫荧光染色切片进行对比。结果随着冷驯化时间延长,白色脂肪细胞和褐色脂肪细胞形态都明显变小,且褐色脂肪组织中解偶联蛋白1(uncoupling protein 1,UCP1)表达增加,同时腹部皮下白色脂肪组织中可能已发生褐变,产生一些具有UCP1表达的浅褐色脂肪细胞,即米色脂肪细胞。结论不同染色切片不仅能够清晰观察到脂肪细胞的形态改变,还能从抗体阳性颜色中观察出UCP1表达变化,为中缅树鼩脂肪组织研究提供基础性材料。
文摘AIM:To investigate morphological changes of intestinal smooth muscle contractile fibres in small bowel atresia patients.METHODS:Resected small bowel specimens from small bowel atresia patients(n = 12) were divided into three sections(proximal,atretic and distal).Standard histology hematoxylin-eosin staining and enzyme immunohistochemistry was performed to visualize smooth muscle contractile markers-smooth muscle actin(SMA) and desmin using conventional paraffin sections of the proximal and distal bowel.Small bowel from agematched patients(n = 2) undergoing Meckel's diverticulum resection served as controls.RESULTS:The smooth muscle coat in the proximal bowel of small bowel atresia patients was thickened compared with control tissue,but the distal bowel was unchanged.Expression of smooth muscle contractile fibres SMA and desmin within the proximal bowel was slightly reduced compared with the distal bowel and control tissue.There were no major differences in the architecture of the smooth muscle within the proximal bowel and the distal bowel.The proximal and distal bowel in small bowel atresia patients revealed only minimal differences regarding smooth muscle morphology and the presence of smooth muscle contractile filament markers.CONCLUSION:Changes in smooth muscle contractile filaments do not appear to play a major role in postoperative motility disorders in small bowel atresia.
