An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the U...An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus.展开更多
Seven McAbs raised against human sperm were evaluated by several immunological assays. The results are shown as follows: (1) Among 7 MeAbs, 5 were IgG1, 2 were IgM.(2) By immunofluorescent assay, McAb 21-2-4 was local...Seven McAbs raised against human sperm were evaluated by several immunological assays. The results are shown as follows: (1) Among 7 MeAbs, 5 were IgG1, 2 were IgM.(2) By immunofluorescent assay, McAb 21-2-4 was localized on postacrosome, neck and midpiece of human sperm; other 5 McAbs were bound to plasma membrane and acrosome; another McAb gave a negative reaction. In addition, when McAbs were reacted with sperms of other species, positive results were also obtained, but with different patterns of staining. It is suggested that the location of the same antigen on sperms of various species may be different. (3) Five McAbs showed mixed pattern of agglutination in TAT, other two displayed tail-tail pattern; three McAbs gave pasitive test in SIT, two revealed weak positive reaction. (4) Cross-reactivity of seven McAbs was examined with seven human somatic tissues as well as peripheral blood lymphocytes, three McAbs reacted with lymphocytes of spleen, thymus and peripheral blood. (5) The reaction of McAbs with testes of various species was observed. McAb 21-2-4 reacted with germ cells of rabbit and hamster, and A 14 with germ cells of rat and hamster. Negative reaction of these two MeAbs with human testes was obtained, but they did react with ejaculatory, sperms, it is suggested that antigens reacted with McAbs probably, were coating antigens.展开更多
Objective： To observe the expression and distribution of adult rat axon guidance cues Netrin- 1 and Slit2 at different time points after spinal cord injury and to investigate the guidance mechanism of regenerated axo...Objective： To observe the expression and distribution of adult rat axon guidance cues Netrin- 1 and Slit2 at different time points after spinal cord injury and to investigate the guidance mechanism of regenerated axons. Methods： Twenty adult Sprague Dawley （SD） rats were divided randomly into five groups with 4 in each. Four groups of them were used to make Allen＇s spinal cord punch models and we took materials randomly from one of them on the 2nd, 4th, 7th and 14th day respectively after operation. The left one group was taken as the control group. Immun- ofluorescence laser confocal scan was used to examine the co-expression and localization of Netrin- 1 and Slit2 proteinsin the injured site of the spinal cord. Results： Within two weeks after SCI, the expression of Netrin- 1 and Slit2 proteins increased temporarily and there was co-expression of them on the neuron plasma membrane. Conclusions： Synchronous high expression and co-expression of axon attractant Netrin-1 and repellent Slit2 are found in the adult rat injured spinal cord in the damaged local and vicinity parts, and probably, they act as the key regulators of axon guidance regeneration.展开更多
Adjuvant monoarthritis was induced by the injection of 50μl Complete Freund's Adjuvant（CFA) into ankle joint of adult SD rats. Electroacupuncture (EA)was performed on Huantiao (GB30)and Yanglingquan (GB 34) (0.5...Adjuvant monoarthritis was induced by the injection of 50μl Complete Freund's Adjuvant（CFA) into ankle joint of adult SD rats. Electroacupuncture (EA)was performed on Huantiao (GB30)and Yanglingquan (GB 34) (0.5-1.5 v, 4-16 Hz, 30 min) after the injection.展开更多
基金National Natural Science Funds(30570081, 30670094 and 30700028)
文摘An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus.
文摘Seven McAbs raised against human sperm were evaluated by several immunological assays. The results are shown as follows: (1) Among 7 MeAbs, 5 were IgG1, 2 were IgM.(2) By immunofluorescent assay, McAb 21-2-4 was localized on postacrosome, neck and midpiece of human sperm; other 5 McAbs were bound to plasma membrane and acrosome; another McAb gave a negative reaction. In addition, when McAbs were reacted with sperms of other species, positive results were also obtained, but with different patterns of staining. It is suggested that the location of the same antigen on sperms of various species may be different. (3) Five McAbs showed mixed pattern of agglutination in TAT, other two displayed tail-tail pattern; three McAbs gave pasitive test in SIT, two revealed weak positive reaction. (4) Cross-reactivity of seven McAbs was examined with seven human somatic tissues as well as peripheral blood lymphocytes, three McAbs reacted with lymphocytes of spleen, thymus and peripheral blood. (5) The reaction of McAbs with testes of various species was observed. McAb 21-2-4 reacted with germ cells of rabbit and hamster, and A 14 with germ cells of rat and hamster. Negative reaction of these two MeAbs with human testes was obtained, but they did react with ejaculatory, sperms, it is suggested that antigens reacted with McAbs probably, were coating antigens.
文摘Objective： To observe the expression and distribution of adult rat axon guidance cues Netrin- 1 and Slit2 at different time points after spinal cord injury and to investigate the guidance mechanism of regenerated axons. Methods： Twenty adult Sprague Dawley （SD） rats were divided randomly into five groups with 4 in each. Four groups of them were used to make Allen＇s spinal cord punch models and we took materials randomly from one of them on the 2nd, 4th, 7th and 14th day respectively after operation. The left one group was taken as the control group. Immun- ofluorescence laser confocal scan was used to examine the co-expression and localization of Netrin- 1 and Slit2 proteinsin the injured site of the spinal cord. Results： Within two weeks after SCI, the expression of Netrin- 1 and Slit2 proteins increased temporarily and there was co-expression of them on the neuron plasma membrane. Conclusions： Synchronous high expression and co-expression of axon attractant Netrin-1 and repellent Slit2 are found in the adult rat injured spinal cord in the damaged local and vicinity parts, and probably, they act as the key regulators of axon guidance regeneration.
文摘Adjuvant monoarthritis was induced by the injection of 50μl Complete Freund's Adjuvant（CFA) into ankle joint of adult SD rats. Electroacupuncture (EA)was performed on Huantiao (GB30)and Yanglingquan (GB 34) (0.5-1.5 v, 4-16 Hz, 30 min) after the injection.
