点腺过路黄免疫调节成分的提取及对小鼠淋巴细胞增殖的影响

目的:观察点腺过路黄提取成分体外对小鼠淋巴细胞增殖的影响。方法:用极性递增的溶剂顺次萃取出全草的脂溶性及水溶性成分,并分别研究它们对小鼠淋巴细胞增殖的影响。结果:氯仿提取部分(10^(-10)～10^(-6)g/ml)可显著增强活化的淋巴细胞的增殖,正丁醇提取部分(10^(-10)～10^(-6)g/ml)可显著抑制活化的B淋巴细胞的增殖,在10^(-6)g/ml时可显著增强活化的T淋巴细胞的增殖,而在10^(-8)～10^(-6)g/ml时则显著抑制活化的T淋巴细胞增殖。结论:点腺过路黄全草的氯仿及正丁醇部分提取物可能具有免疫调节功能。

免疫增强型黑色营养膳的营养评价及其作用研究

将黑黏米、黑豆、黑芝麻经发芽、破碎、挤压膨化、微波灭菌等工序加工成营养膳,按国标规定检测其营养成分含量。将营养膳添加入基础饲料,以20、40、60 g/kg bw3种剂量给予昆明种小鼠。连续喂养30 d后,观察其对小鼠各项免疫功能指标的影响。结果表明以黑黏米、黑豆、黑芝麻为主要原料加工制成的营养膳中蛋白质质量分数达到25.44%,脂肪18.81%,此外维生素和矿物质均较高,8种必需氨基酸均高出WHO/FAO模式标准。在免疫调节试验中,营养膳能增强小鼠的胸腺指数和脾指数,提升淋巴细胞刺激指数和小鼠NK细胞活性,提高腹腔巨噬细胞的吞噬功能和溶血素含量,其最适作用剂量为40 g/kg bw。

抗癌鲜药的生物治疗作用

现代鲜药（金龙胶囊、金水鲜胶囊），是选用鲜活动物、植物药材为原料组方，以现代低温技术，提取其全部有效成分用于恶性肿瘤治疗的中药制剂。创新的制备工艺是我国中药鲜药应用史上的革命。

Recent advances in cytokines:Therapeutic implications for inflammatory bowel diseases

Inflammatory bowel diseases （IBDs） are complex and chronic disabling conditions resulting from a dysregulated dialogue between intestinal microbiota and components of both the innate and adaptive immune systems. Cytokines are essential mediators between activated immune and non-immune cells, including epithelial and mes- enchymal cells. They are immunomodulatory peptides released by numerous cells and these have significant effects on immune function leading to the differentiation and survival of T cells. The physiology of IBD is becom- ing a very attractive field of research for development of new therapeutic agents. These include cytokines involved in intestinal immune inflammation. This review will focus on mechanisms of action of oytokines involved in IBD and new therapeutic opportunities for these diseases.

Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth

AIM： To investigate the functional significance of insulin-like growth factor binding protein-5 （IGFBP-5） overexpression in pancreatic cancer （PaC）.METHODS： The effects of IGFBP-5 on cell growth were assessed by stable transfection of BxPC-3 and PANC-1 cell lines and measuring cell number and DNA synthesis. Alterations in the cell cycle were assessed by flow cytometry and immunoblot analyses. Changes in cell survival and signal transduction were evaluated after mitogen and phosphatidylinositol activated protein kinase 3-kinase （PI3K） inhibitor treatment.RESULTS： After serum deprivation, IGFBP-5 expression increased both cell number and DNA synthesis in BxPC-3 cells, but reduced cell number in PANC-1 cells. Consistent with this observation, cell cycle analysis of IGFBP-5-expressing cells revealed accelerated cell cycle progression in BxPC-3 and G2/M arrest of PANC-1 cells. Signal transduction analysis revealed that Akt activation was increased in BxPC-3, but reduced in PANC-1 cells that express IGFBP-5. Inhibition of PI3K with LY294002 suppressed extracellular signal-regulated kinase-1 and -2 （ERK1/2） activation in BxPC-3, but enhanced ERK1/2 activation in PANC-1 cells that express IGFBP-5. When MEK1/2 was blocked, Akt activation remained elevated in IGFBP-5 expressing PaC cells; however, inhibition of PI3K or MEK1/2 abrogated IGFBP-5-mediated cell survival.CONCLUSION： These results indicate that IGFBP-5 expression affects the cell cycle and survival signal pathways and thus it may be an important mediator of PaC cell growth.

保健咨询台

连日来，本刊收到一些读者来信，咨询有关“参百益——Rg3”方面的知识。为此，我们请教了有关专家，现做以下回答。