母婴间营养及免疫指标的探讨

目的 检测前白蛋白 (PA)作为孕妇及新生儿营养指标的价值及与免疫球蛋白IgG和微量元素锌 (Zn)的关系。方法 选择 188对孕妇及新生儿 ,其中足月儿 14 8例 ,早产儿 4 0例 ,检测孕妇血清及新生儿脐血PA、白蛋白 (Alb)、总蛋白(TP)、免疫球蛋白IgG(单向免疫扩散法 )及微量元素Zn(WFD -Y2 型原子吸收分光光度计火焰法 )水平 ,并进行相关分析。结果 (1)足月儿PA、Alb、TP、IgC和Zn均显著高于早产儿 (PPA <0 0 0 1,P Ab <0 0 1,PTP <0 0 0 1,PIgG <0 0 0 1,Pzn <0 0 1)。 (2 )脐血PA低于母亲血清PA(P <0 0 0 1) ,足月儿脐血IgG高于母亲血清IgG(P <0 0 5 ) ,脐血Zn显著高于孕妇血清Zn(P <0 0 0 1)。 (3)新生儿PA与体重、Alb、TP及IgG均呈正相关 (rW =0 2 13,rAIb=0 198,rTP=0 2 14 ,rIgG=0 186 ,P均 <0 0 5 ) ,Zn与PA、IgG无相关性。结论 (1)PA可为孕妇及新生儿更准确、更敏感的营养指标。 (2 )PA水平可反映新生儿体液免疫状态及胎盘功能。 (3)为全面正确评价新生儿营养及免疫状态 ,应同时检测PA、IgG和Zn。

Effect of moxibustion at Shenque (CV8) on immune system in rats with different degrees of exhaustive exercise

Objective: To investigate the effect of moxibustion at Shenque (CV8) on the immune system in rats with different levels of exhaustive exercise. Methods: Fifty-six male Sprague-Dawley (SD) rats were randomly divided into a blank group (n=8), an exhaustive group (n=24), and a moxibustion group (n=24). The exhaustive group was randomly divided into a 1-time exhaustive group, a 4-time exhaustive group and a 7-time exhaustive group, with 8 rats in each group. According to the treatment time, the moxibustion group was randomly divided into a 1-time moxibustion group, a 4-time moxibustion group and a 7-time moxibustion group, with 8 rats in each group. Rats in the exhaustive groups and the moxibustion groups were subjected to replicating the exhaustive swimming models. Rats in each moxibustion group received mild moxibustion for 15 min immediately after the exhaustive modeling, once every other day. Twenty-four hours after the corresponding exhaustive exercise, the rats in each group were tested for the levels of serum immunoglobulin (Ig) G, IgA, IgM and acid phosphatase (ACP), and the morphological changes of spleen tissues were observed. The level of IgA was detected by immunoturbidimetric assay, and the levels of IgG, IgM and ACP were detected by en zyme-linked imm uno sorb ent assay (ELISA). Results: Compared with the 1-time exhaustive group, swimming time of rats in the 4-time exhaustive group was significantly proIonged (P<0.01), and swimming time of rats in the 7-time exhaustive group was significantly shortened (P<0.01). Compared with the 7-time exhaustive group, exhaustive swimming time of rats in the 7-time moxibustion group was significantly proIonged (P<0.01). Compared with the blank group, the IgG level in the 1-time exhaustive group was significantly decreased (PvO.Ol), and the levels of IgG, IgA and IgM in the 4-time exhaustive group and the 7-time exhaustive group were all sign ifica ntly decreased (P<0.05 or PvO.Ol), while the ACP level was in creased sign ifica ntly (both P<0.01). Microscopically, the number of splenic corpuscles in the 1-time exhaustive group was reduced;the center of some splenic corpuscles in the 4-time exhaustive group was damaged;the number of splenic corpuscles in the 7-time exhaustive group was reduced, and there was no obvious germinal center. Compared with the 4-time exhaustive group, the IgA level in the 4-time moxibusti on group was sign ifica ntly in creased (P<0.01), and the ACP level was sign ifica ntly decreased (P<0.01). Compared with the 7-time exhaustive group, the levels of IgG, IgA and IgM in the 7-time moxibustion group were sign ifica ntly in creased (all PvO.Ol), and the ACP level was sign ifica ntly decreased (P<0.01). Microscopically, the nu mber of splenic corpuscles in the 1-time moxibustion group was reduced;the center of some splenic corpuscles in the 4-time moxibustion group was damaged together with hyperplasia of some splenic corpuscles;blast cells were proliferated in the center of some splenic corpuscles in the 7-time moxibustion group. Conclusion: Moxibustion at Shenque (CV 8) can improve the levels of IgG, IgA and IgM, reduce the ACP level, repair damaged spleen tissues, and enhance the immunity of the body to some extent in the Iong-term fatigue rats.

Effect of mild-warm moxibustion on fatigue, immunestate and quality of life in sub-health population

Objective： To observe the effect of mild-warm moxa stick moxibustion on fatigue, immune state and quality of life （QOL） in sub-health population. Methods： A total of 120 cases were randomly allocated into a moxibustion group （n=60） and a Chinese patent medicine （CPM） group （n=60）. People in the moxibustion group were intervened with mild-warm moxa stick moxibustion, once a day; while people in the control group took oral Bu Zhong Yi Qi Won （Spleen/stomach-supplementing and Qi-boosting Pill）, 3 doses a day. Two months made up a course of treatment. The fatigue, immune state and QOL were then observed after one treatment course, followed by a 12-month follow-up. Results： A total of 215 cases completed the clinical trial. After intervention, the fatigue scores, immune parameters and QOL scores were significantly increased in both groups, showing statistical significances （P〈0.05）. There were between-group significant differences in fatigue score, levels of immunoglobulin M （IgM） and IgG, natural killer （NK） cell activity and QOL score （P〈O.05）. After the 22-month follow-up, there were still significant between-group differences in fatigue score, physical health, psychological, social relationships and overall perception of QOL and overall perception of health （P〈0.05）. Conclusion： Mild-warm moxibustion can markedly improve the fatigue, immune state and QOL in sub-health population.