Objective：To explore the expression of nuclear factor-kappa B （NF-kB） in Schwann cells （SCs） and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods： Thirty...Objective：To explore the expression of nuclear factor-kappa B （NF-kB） in Schwann cells （SCs） and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods： Thirty-six adult Sprague-Dawley （SD） rats were divided randomly into normal control group （n=6）, and sciatic nerves crushing group （n= 30）, and the later was further equally randomized into 5 subgroups： 1, 3, 7, 14, and 21 d post-injury groups. The expression of NF-kB of normal and injured nerves were examined by immunohistochemistry staining, and the apoptosis of motor neurons in spinal cord of lumbar 4 to lumbar 6 （L4-L6） was investigated by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling （TUNEL） assay. Both were qua.ntitated by image analysis. Results： In crushing group, except 21 d post-injury group, the expression of NF-kB was markedly higher than that in the normal control group （P〈0.05, P〈0. 01）. At 1 d after sciatic nerves crushing, the expression of NF-kB was obviously up-regulated, reached peak at 3 d, and recovered at 21 d. The same trend was observed in the time-course on motor neuron apoptosis after sciatic nerves injury. Correlation analyses revealed that motor neuron apoptosis was significantly and positively correlated with the expression of NF-kB following sciatic nerves injury （r= 0. 976 0, P〈0. 01）. Conclusion： After injury of sciatic nerves, the presence and up-regulation of NF-kB in SCs may be involved in motor neuron apoptosis in L4-L6 spinal cord.展开更多
The present study presents a peripheral nerve injury animal model for evaluation of an implantable electrical stimulator we designed.The evaluation was confirmed by ethological,electrophysiological and histologic stud...The present study presents a peripheral nerve injury animal model for evaluation of an implantable electrical stimulator we designed.The evaluation was confirmed by ethological,electrophysiological and histologic study.Twenty New Zealand rabbits were used.The left sciatic nerve was crushed with a micro-vessel clamp in all rabbits,and the stimulators were implanted in ten rabbits.The other ten rabbits were in a control group.As compared with the implantation group,3 and 6 weeks after operation,the histology showed the typical pathologic atrophy by hematoxylin-eosin(HE) ,the motor nerve conduction velocity(MCV) was found significantly slowed and the axon of crushed nerve at distal portion exhibited breakdown in the control group.These results reveal that the implantable electrical stimulator was effective and was suitable for implantation in an animal model.展开更多
基金Supported by the National Natural Science Foundation of China (No. 30600665)the Opening Project Foundation of State Key Laboratory of Trauma. Burns and Combined Injury (No. 2006A-3)the Youth Scientific Research Foundation of Third Military Medical University (No.06XG048)
文摘Objective：To explore the expression of nuclear factor-kappa B （NF-kB） in Schwann cells （SCs） and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods： Thirty-six adult Sprague-Dawley （SD） rats were divided randomly into normal control group （n=6）, and sciatic nerves crushing group （n= 30）, and the later was further equally randomized into 5 subgroups： 1, 3, 7, 14, and 21 d post-injury groups. The expression of NF-kB of normal and injured nerves were examined by immunohistochemistry staining, and the apoptosis of motor neurons in spinal cord of lumbar 4 to lumbar 6 （L4-L6） was investigated by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling （TUNEL） assay. Both were qua.ntitated by image analysis. Results： In crushing group, except 21 d post-injury group, the expression of NF-kB was markedly higher than that in the normal control group （P〈0.05, P〈0. 01）. At 1 d after sciatic nerves crushing, the expression of NF-kB was obviously up-regulated, reached peak at 3 d, and recovered at 21 d. The same trend was observed in the time-course on motor neuron apoptosis after sciatic nerves injury. Correlation analyses revealed that motor neuron apoptosis was significantly and positively correlated with the expression of NF-kB following sciatic nerves injury （r= 0. 976 0, P〈0. 01）. Conclusion： After injury of sciatic nerves, the presence and up-regulation of NF-kB in SCs may be involved in motor neuron apoptosis in L4-L6 spinal cord.
基金the Shanghai-Philips Research & Development Fund of China (No.06SP07001)Shanghai Rising-Star Program (No.08QH14015)
文摘The present study presents a peripheral nerve injury animal model for evaluation of an implantable electrical stimulator we designed.The evaluation was confirmed by ethological,electrophysiological and histologic study.Twenty New Zealand rabbits were used.The left sciatic nerve was crushed with a micro-vessel clamp in all rabbits,and the stimulators were implanted in ten rabbits.The other ten rabbits were in a control group.As compared with the implantation group,3 and 6 weeks after operation,the histology showed the typical pathologic atrophy by hematoxylin-eosin(HE) ,the motor nerve conduction velocity(MCV) was found significantly slowed and the axon of crushed nerve at distal portion exhibited breakdown in the control group.These results reveal that the implantable electrical stimulator was effective and was suitable for implantation in an animal model.
