目的实验研究大剂量左氧氟沙星对兔肾功能的影响。方法将16只新西兰白兔随机分为四组:4只作为对照组,余按时间分为静脉滴注左氧氟沙星后1h组、2h组、3h组,每组4只。对照组给予生理盐水,其余3组均给于左氧氟沙星0.05g/kg,药物溶于50m l 0...目的实验研究大剂量左氧氟沙星对兔肾功能的影响。方法将16只新西兰白兔随机分为四组:4只作为对照组,余按时间分为静脉滴注左氧氟沙星后1h组、2h组、3h组,每组4只。对照组给予生理盐水,其余3组均给于左氧氟沙星0.05g/kg,药物溶于50m l 0.9%N aC l溶液中。各组分别在给药后1、2和3h时应用彩色多普勒超声观察兔肾的大小、形态及内部回声的改变,应用彩色多普勒能量图及彩色血流观察兔肾血流灌注情况并测量兔肾动脉的血流参数。彩超观察完毕后于耳缘静脉在各个时点取血做生化检验,最后福尔马林灌注处死取材切片,使用光镜观察兔肾脏的形态学变化。结果左氧氟沙星组兔肾大小、内部回声、血流灌注及血流参数均无明显变化,各种生化指标均无明显变化,与对照组比较无统计学意义(P>0.05),光镜下左氧氟沙星组兔肾组织无明显异常。结论大剂量左氧氟沙星对兔肾功能无明显影响。展开更多
Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hy...Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hydric restriction times （2 h and 4 h per day, continuously） were used in our trial carried in good sanitary conditions. Thirty six rabbits were divided at weaning （28 days） into three groups： R2 with restricted access to drinking water from 35 days to 80 days age of 2 h per day （from 8 a.m. to 10 a.m.）, R4： access to drinking water 4 h per day （from 8 a.m. to 12 a.m.） and T （control group） with unlimited access to drinking water. Six animals from each group were slaughtered at 80 days of age. Carcass measurements and meat quality were studied. Histological study for kidneys was carried out. Relative weight of gastrointestinal tract increases with the degree of restriction： 16.5, 18.4 and 18.9 for T, R4 and R2, respectively （P 〈 0.05）. Perirenal fat was lower in restricted groups but differences were not statically significant. Scapular fat decreases of 2/3 or 1/3 respectively with limiting access to drinking water of 2 h or 4 h per day. Compared to control group （T）, meat of restricted groups contains more water： 70.3%, 70% and 69.3% respectively for R4, R2 and T （P 〈 0.01）. The same tendency was observed for proteins： 19.2%, 19.3% and 17.7% for groups R2, R4 and T. Fat decreases in meat of restricted animals： 8.4%, 8.2% and 8.1% respectively for T, R4 and R2 （P 〈 0.05）. Water restriction induced decrease of carcass and meat adiposity. There were no differences between pH post mortem and pH ultimate （after 24 h） between the three groups. Hydric restriction does not effect on kidney histology.展开更多
文摘目的实验研究大剂量左氧氟沙星对兔肾功能的影响。方法将16只新西兰白兔随机分为四组:4只作为对照组,余按时间分为静脉滴注左氧氟沙星后1h组、2h组、3h组,每组4只。对照组给予生理盐水,其余3组均给于左氧氟沙星0.05g/kg,药物溶于50m l 0.9%N aC l溶液中。各组分别在给药后1、2和3h时应用彩色多普勒超声观察兔肾的大小、形态及内部回声的改变,应用彩色多普勒能量图及彩色血流观察兔肾血流灌注情况并测量兔肾动脉的血流参数。彩超观察完毕后于耳缘静脉在各个时点取血做生化检验,最后福尔马林灌注处死取材切片,使用光镜观察兔肾脏的形态学变化。结果左氧氟沙星组兔肾大小、内部回声、血流灌注及血流参数均无明显变化,各种生化指标均无明显变化,与对照组比较无统计学意义(P>0.05),光镜下左氧氟沙星组兔肾组织无明显异常。结论大剂量左氧氟沙星对兔肾功能无明显影响。
文摘Feed restriction is a common practice to reduce post weaning digestive disorders in rabbits and to get carcasses with less fat. Hydric restriction is commonly used to induce feed restriction in growing rabbits. Two hydric restriction times （2 h and 4 h per day, continuously） were used in our trial carried in good sanitary conditions. Thirty six rabbits were divided at weaning （28 days） into three groups： R2 with restricted access to drinking water from 35 days to 80 days age of 2 h per day （from 8 a.m. to 10 a.m.）, R4： access to drinking water 4 h per day （from 8 a.m. to 12 a.m.） and T （control group） with unlimited access to drinking water. Six animals from each group were slaughtered at 80 days of age. Carcass measurements and meat quality were studied. Histological study for kidneys was carried out. Relative weight of gastrointestinal tract increases with the degree of restriction： 16.5, 18.4 and 18.9 for T, R4 and R2, respectively （P 〈 0.05）. Perirenal fat was lower in restricted groups but differences were not statically significant. Scapular fat decreases of 2/3 or 1/3 respectively with limiting access to drinking water of 2 h or 4 h per day. Compared to control group （T）, meat of restricted groups contains more water： 70.3%, 70% and 69.3% respectively for R4, R2 and T （P 〈 0.01）. The same tendency was observed for proteins： 19.2%, 19.3% and 17.7% for groups R2, R4 and T. Fat decreases in meat of restricted animals： 8.4%, 8.2% and 8.1% respectively for T, R4 and R2 （P 〈 0.05）. Water restriction induced decrease of carcass and meat adiposity. There were no differences between pH post mortem and pH ultimate （after 24 h） between the three groups. Hydric restriction does not effect on kidney histology.
