Aim To establish a sensitive and specific liquid chromatography-massspeetrometry method for determination of scutellarin in rabbit plasma after oral administration.Methods For the quantitative analysis, rutin was used...Aim To establish a sensitive and specific liquid chromatography-massspeetrometry method for determination of scutellarin in rabbit plasma after oral administration.Methods For the quantitative analysis, rutin was used as an internal standard and solid-phaseextraction (SPE) was performed by using a Phenomenex C_8 cartridge. HPLC was carried out using aZorbax Extend-C_(18) column (150 mm x 2.1 mm ID, 5 μm) with a guard cartridge (Phenomenex) .Gradient elution was selected with the mobile phase of methanol 10 mmol·L^(-1) ammonium acetatesolution (pH adjusted to 8.0 with ammonia solution). The flow rate of mobile phase was 0.4mL·min^(-1) and the column temperature was 35 ℃ . Both scutellarin and the internal standard rutinin rabbit plasma extracts were detected by mass spectrometry using an ESI interface in the negativeion mode. Results The linear range was from 2 to 200 ng· mL^(-1), with acceptable accuracy andprecision (RSD) . Conclusion A sensitive, reliable and accurate method for the quantitation ofscutellarin in rabbit plasma has been established.展开更多
This paper describes a kinetic spectrophotometric method for simultaneous determination of three cephalosporin antibiotics,cephradine,cefaclor and cefixime,with the aid of chemometrics. The method relies on their oxid...This paper describes a kinetic spectrophotometric method for simultaneous determination of three cephalosporin antibiotics,cephradine,cefaclor and cefixime,with the aid of chemometrics. The method relies on their oxidation with KMnO4 to produce green manganate with different kinetic rates in alkaline medium. The proposed method was successfully applied to a pharmacokinetic study of three cephalosporin antibiotics in rabbit plasma via intravenous injections. The results indicated that the amount of cephradine,cefaclor and cefixime were reduced rapidly in rabbit blood,showing clear dose-dependency and the half-life of cefixime (160 min) was longer than those of cephradine (60 min) and cefaclor (60 min).展开更多
文摘Aim To establish a sensitive and specific liquid chromatography-massspeetrometry method for determination of scutellarin in rabbit plasma after oral administration.Methods For the quantitative analysis, rutin was used as an internal standard and solid-phaseextraction (SPE) was performed by using a Phenomenex C_8 cartridge. HPLC was carried out using aZorbax Extend-C_(18) column (150 mm x 2.1 mm ID, 5 μm) with a guard cartridge (Phenomenex) .Gradient elution was selected with the mobile phase of methanol 10 mmol·L^(-1) ammonium acetatesolution (pH adjusted to 8.0 with ammonia solution). The flow rate of mobile phase was 0.4mL·min^(-1) and the column temperature was 35 ℃ . Both scutellarin and the internal standard rutinin rabbit plasma extracts were detected by mass spectrometry using an ESI interface in the negativeion mode. Results The linear range was from 2 to 200 ng· mL^(-1), with acceptable accuracy andprecision (RSD) . Conclusion A sensitive, reliable and accurate method for the quantitation ofscutellarin in rabbit plasma has been established.
基金support from the National Natural Science Foundation of China (21065007)the State Key Labo-ratory of Food Science and Technology of Nanchang University (SKLF-MB-200807 & SKLF-TS-200919)
文摘This paper describes a kinetic spectrophotometric method for simultaneous determination of three cephalosporin antibiotics,cephradine,cefaclor and cefixime,with the aid of chemometrics. The method relies on their oxidation with KMnO4 to produce green manganate with different kinetic rates in alkaline medium. The proposed method was successfully applied to a pharmacokinetic study of three cephalosporin antibiotics in rabbit plasma via intravenous injections. The results indicated that the amount of cephradine,cefaclor and cefixime were reduced rapidly in rabbit blood,showing clear dose-dependency and the half-life of cefixime (160 min) was longer than those of cephradine (60 min) and cefaclor (60 min).
