From the roots of Aristolochia triangularis, two new alkaloids triangularine A and triangularine B together with thirteen known alkaloids namely aristololactam AII, AIIIa, AIA, Ia, IIA, BII, CII, cepharadione A,...From the roots of Aristolochia triangularis, two new alkaloids triangularine A and triangularine B together with thirteen known alkaloids namely aristololactam AII, AIIIa, AIA, Ia, IIA, BII, CII, cepharadione A, 4,5 dioxodehydroasimilobine; aristolochic acid D (IVa), magnoflorine; N, N dimethyllindcarpine and oblongine were isolated, their structures were identified by means of spectral analysis and chemical conversion.展开更多
Aristolochic acids （AAs）, a natural mixture of 8-methoxy-6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAI）and 6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAII）, derived from aristo...Aristolochic acids （AAs）, a natural mixture of 8-methoxy-6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAI）and 6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAII）, derived from aristolochiaceae species, has beenreported to cause AAS-induced nephropathy and upper urothelial cancer. In this review, we summarize the informationon the nephrotoxicity and carcinogenesis of AAs and their derivatives. AAs nephrotoxicity can lead to apoptosis andoxidative stress of renal tubular cells, and inhibition of the expression of aquaporins. AAs can also reduce the capabilityfor renal tubular epithelial cell repair after acute injury and further produce renal fibrosis by activating TGF-β-Smadsignaling and promoting the migration of macrophages. Moreover, AAs-induced carcinogenesis may be due to theformation of covalent adducts with DNA which can lead to the mutation in certain tumor suppressor genes orproto-oncogenes and the different catalyzing capacity of the microsomal cytochrome P450 of individuals in AAImetabolism.展开更多
Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present s...Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present study, we established an ultra high performance liquid chromatography （UHPLC） coupled with quadmpole/time-of-flight mass spectrometry （Q/TOF-MS） method for the rapid analysis of eight AA analogues in 19 samples originated from the five Aristolochiaceae plants, the roots and rhizomes ofAsarum sieboldii Miq. var. seoulense Nakai, the fruits ofAristolochia contorta Bunge or A. debilis Sieb. et Zucc., the roots ofAristolochia debilis Sieb. et Zucc., the stems ofAristolochia manshuriensis Kom., and the roots of Aristolochiafangchi Y. C. Wu ex L. D. Chou et S. M. Hwang. A total of five AAs and three ALs were identified by co-chromatography of sample extract and comparing the retention time, UV spectra, and characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification or semi-quantification of them. The samples significantly differed in the quality and quantity of AA analogues, which aUowed the possibility of showing their chemical distinctness, and it might be helpful in their standardization and quality control. Furthermore, in order to holistically compare the difference between the five Aristolochiaceae plants, dataset obtained from UHPLC-Q/TOF-MS was processed with principal component analysis （PCA） and orthogonal partial least squared discriminant analysis （OPLS-DA）.展开更多
Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectr...Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectroscopic analysis. Thirteen compounds were obtained and identified as 7-methoxyl-aristololactam Ⅳ （1）, aristololactam Ⅱ （2）, aristolochic acid Ⅰ （3）, aristololactam AⅡ （4）, daucosterol （5）, aristololactam Ⅰa （6）, N-trans-feruloyl tyramine （7）, aristololactam Ⅰ （8）, 4β,10β-aromadendranediol （9）, aristololide （10）, aristolic acid Ⅰ （11）, meso-dihydroguaiaretic acid （12）, and calopiptin （13）. These compounds were obtained from the genus Saruma for the first time, and they provided chemical evidences for the chemotaxonomy of plants of the Aristolochiaceae family. Since aristolochic acids and aristololactams are toxic to kidney, the results of this investigation suggest that it should be cautious to use Saruma henryi as a medicine.展开更多
The pharmacokinetic parameters of aristolochic acid-Ⅰ （AA-Ⅰ） and -Ⅱ （AA-Ⅱ） in rat serum after intragastrical administration of the crude drug powders of Radix Aristolochiae （RA） and Muskone containing equal ...The pharmacokinetic parameters of aristolochic acid-Ⅰ （AA-Ⅰ） and -Ⅱ （AA-Ⅱ） in rat serum after intragastrical administration of the crude drug powders of Radix Aristolochiae （RA） and Muskone containing equal amounts of RA were compared. The pharmacokinetic profiles of AA-Ⅰ and AA-Ⅱ could be fitted with a two-compartment model The elimination half time （T1/2β） of AA-Ⅰ in Muskone was 1573.2 min and that of AA-Ⅰ in RA was 475.8 min; T1/2β of AA-Ⅱ in Muskone was 2344.8 min and that of AA-Ⅱ in RA was 427.8 rain. The area under the concentration-time curve （AUC） of AA-Ⅰ in Muskone was 13.07 μg/h/mL and that of AA-Ⅰ in RA was 3.86 μg/h/mL; AUC of AA-Ⅱ in Muskone was 67.67 μg/h/mL and that of AA-Ⅱ in RA was 23.93 μg/h/mL. The bioavailabilities of AA-Ⅰ and AA-Ⅱ in Muskone were markedly increased compared with that in RA based on the elimination half-time and A UC values.展开更多
文摘From the roots of Aristolochia triangularis, two new alkaloids triangularine A and triangularine B together with thirteen known alkaloids namely aristololactam AII, AIIIa, AIA, Ia, IIA, BII, CII, cepharadione A, 4,5 dioxodehydroasimilobine; aristolochic acid D (IVa), magnoflorine; N, N dimethyllindcarpine and oblongine were isolated, their structures were identified by means of spectral analysis and chemical conversion.
文摘Aristolochic acids （AAs）, a natural mixture of 8-methoxy-6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAI）and 6-nitro-phenanthro-（3,4-d）-1,3-dioxolo-5-carboxylic acid （AAII）, derived from aristolochiaceae species, has beenreported to cause AAS-induced nephropathy and upper urothelial cancer. In this review, we summarize the informationon the nephrotoxicity and carcinogenesis of AAs and their derivatives. AAs nephrotoxicity can lead to apoptosis andoxidative stress of renal tubular cells, and inhibition of the expression of aquaporins. AAs can also reduce the capabilityfor renal tubular epithelial cell repair after acute injury and further produce renal fibrosis by activating TGF-β-Smadsignaling and promoting the migration of macrophages. Moreover, AAs-induced carcinogenesis may be due to theformation of covalent adducts with DNA which can lead to the mutation in certain tumor suppressor genes orproto-oncogenes and the different catalyzing capacity of the microsomal cytochrome P450 of individuals in AAImetabolism.
基金The National Science and Technology Support Program during the Twelfth Five-Year Plan of China(Grant No.2011BAI03B05)the Distinguished Professor Foundation of Liaoning Province of China of 2011Innovative Drug Incubation Base Plan Project from Liaoning Province of China of 2013(Grant No.2013226027)
文摘Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present study, we established an ultra high performance liquid chromatography （UHPLC） coupled with quadmpole/time-of-flight mass spectrometry （Q/TOF-MS） method for the rapid analysis of eight AA analogues in 19 samples originated from the five Aristolochiaceae plants, the roots and rhizomes ofAsarum sieboldii Miq. var. seoulense Nakai, the fruits ofAristolochia contorta Bunge or A. debilis Sieb. et Zucc., the roots ofAristolochia debilis Sieb. et Zucc., the stems ofAristolochia manshuriensis Kom., and the roots of Aristolochiafangchi Y. C. Wu ex L. D. Chou et S. M. Hwang. A total of five AAs and three ALs were identified by co-chromatography of sample extract and comparing the retention time, UV spectra, and characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification or semi-quantification of them. The samples significantly differed in the quality and quantity of AA analogues, which aUowed the possibility of showing their chemical distinctness, and it might be helpful in their standardization and quality control. Furthermore, in order to holistically compare the difference between the five Aristolochiaceae plants, dataset obtained from UHPLC-Q/TOF-MS was processed with principal component analysis （PCA） and orthogonal partial least squared discriminant analysis （OPLS-DA）.
基金National Natural Science Foundation of China (Grant No. 30371748)the 985 Project of Peking University and the National Eleventh Five-year Key Technologies R & D Program of China (Grant No. 2006BAI14B01)
文摘Chemical constituents of the whole herb of Saruma henryi Oliv. were investigated. The herbal extract was separated by repeated column chromatography over silica gel and celite. The structures were elucidated by spectroscopic analysis. Thirteen compounds were obtained and identified as 7-methoxyl-aristololactam Ⅳ （1）, aristololactam Ⅱ （2）, aristolochic acid Ⅰ （3）, aristololactam AⅡ （4）, daucosterol （5）, aristololactam Ⅰa （6）, N-trans-feruloyl tyramine （7）, aristololactam Ⅰ （8）, 4β,10β-aromadendranediol （9）, aristololide （10）, aristolic acid Ⅰ （11）, meso-dihydroguaiaretic acid （12）, and calopiptin （13）. These compounds were obtained from the genus Saruma for the first time, and they provided chemical evidences for the chemotaxonomy of plants of the Aristolochiaceae family. Since aristolochic acids and aristololactams are toxic to kidney, the results of this investigation suggest that it should be cautious to use Saruma henryi as a medicine.
基金The 10 th Five Years Programs for Science and Technology Development of China(Grant No. 2004BA721A10)National Natural Science Foundation of China (Grant No.30371748)
文摘The pharmacokinetic parameters of aristolochic acid-Ⅰ （AA-Ⅰ） and -Ⅱ （AA-Ⅱ） in rat serum after intragastrical administration of the crude drug powders of Radix Aristolochiae （RA） and Muskone containing equal amounts of RA were compared. The pharmacokinetic profiles of AA-Ⅰ and AA-Ⅱ could be fitted with a two-compartment model The elimination half time （T1/2β） of AA-Ⅰ in Muskone was 1573.2 min and that of AA-Ⅰ in RA was 475.8 min; T1/2β of AA-Ⅱ in Muskone was 2344.8 min and that of AA-Ⅱ in RA was 427.8 rain. The area under the concentration-time curve （AUC） of AA-Ⅰ in Muskone was 13.07 μg/h/mL and that of AA-Ⅰ in RA was 3.86 μg/h/mL; AUC of AA-Ⅱ in Muskone was 67.67 μg/h/mL and that of AA-Ⅱ in RA was 23.93 μg/h/mL. The bioavailabilities of AA-Ⅰ and AA-Ⅱ in Muskone were markedly increased compared with that in RA based on the elimination half-time and A UC values.
