[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobac...[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.展开更多
Even though various wireless Net- work Access Technologies （NATs） with dif- ferent specifications and applications have been developed in the recent years, no single wireless technology alone can satisfy the any- ti...Even though various wireless Net- work Access Technologies （NATs） with dif- ferent specifications and applications have been developed in the recent years, no single wireless technology alone can satisfy the any- time, anywhere, and any service wire- less-access needs of mobile users. A real seamless wireless mobile environment is only realized by considering vertical and horizontal handoffs together. One of the major design issues in heterogeneous wireless networks is the support of Vertical Handoff （VHO）. VHO occurs when a multi-interface enabled mobile terminal changes its Point of Attachment （PoA） from one type of wireless access technology to another, while maintaining an active session. In this paper we present a novel multi-criteria VHO algorithm, which chooses the target NAT based on several factors such as user preferences, system parameters, and traf- tic-types with varying Quality of Service （QoS） requirements. Two modules i.e., VHO Neces- sity Estimation （VHONE） module and target NAT selection module, are designed. Both modules utilize several ＂weighted＂ users＇ and system＇s parameters. To improve the robust- ness of the proposed algorithm, the weighting system is designed based on the concept of fuzzy linguistic variables.展开更多
基金the Fund of Basis Scientific Research Operation of Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciencesthe Grant of Scientific Fund of Chinese Academy of Tropical Agricultural Sciences (NoRky0529)~~
文摘[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.
文摘Even though various wireless Net- work Access Technologies （NATs） with dif- ferent specifications and applications have been developed in the recent years, no single wireless technology alone can satisfy the any- time, anywhere, and any service wire- less-access needs of mobile users. A real seamless wireless mobile environment is only realized by considering vertical and horizontal handoffs together. One of the major design issues in heterogeneous wireless networks is the support of Vertical Handoff （VHO）. VHO occurs when a multi-interface enabled mobile terminal changes its Point of Attachment （PoA） from one type of wireless access technology to another, while maintaining an active session. In this paper we present a novel multi-criteria VHO algorithm, which chooses the target NAT based on several factors such as user preferences, system parameters, and traf- tic-types with varying Quality of Service （QoS） requirements. Two modules i.e., VHO Neces- sity Estimation （VHONE） module and target NAT selection module, are designed. Both modules utilize several ＂weighted＂ users＇ and system＇s parameters. To improve the robust- ness of the proposed algorithm, the weighting system is designed based on the concept of fuzzy linguistic variables.
