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旅游模式转变影响下的社会经济效益评价——对鼓浪屿入遗效应的再思考 被引量:2
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作者 吕宁 魏青 《中国文化遗产》 2020年第1期32-40,共9页
世界遗产影响效益分为社会、经济和环境三大类型,而世界遗产的遗产类型、区域基础、基础设施、管理模式、保护政策、发展目标等诸多因素,均会对入遗效应产生影响。详细的数据比对和定期访谈、问卷监测表明,鼓浪屿的申遗和入遗过程对城... 世界遗产影响效益分为社会、经济和环境三大类型,而世界遗产的遗产类型、区域基础、基础设施、管理模式、保护政策、发展目标等诸多因素,均会对入遗效应产生影响。详细的数据比对和定期访谈、问卷监测表明,鼓浪屿的申遗和入遗过程对城市形态、商业业态、社区品质均产生了明显影响。与其他遗产地相比,鼓浪屿由此前游客量至上的旅游模式转变为注重旅游体验与社区需求、平衡保护与发展关系的可持续模式,证明随着遗产理论和文化实践的发展,广受关注的"入遗效应"也有了目的和方向的转变和拓展。 展开更多
关键词 入遗效应 鼓浪屿 世界 旅游模式 可持续发展
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“入遗效应”的理论发展探究
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作者 陈曦 李东会 《中国名城》 2020年第5期19-25,共7页
"入遗效应"曾多次在国际文化遗产会议上被提及,是一项在遗产保护过程中不可忽视的重要课题。首先通过对既往文献的研究,结论分三个主流方向:(1)认为其具有正向影响;(2)认为其具有负向影响;(3)认为其基本无影响或影响相对较小... "入遗效应"曾多次在国际文化遗产会议上被提及,是一项在遗产保护过程中不可忽视的重要课题。首先通过对既往文献的研究,结论分三个主流方向:(1)认为其具有正向影响;(2)认为其具有负向影响;(3)认为其基本无影响或影响相对较小。继而分析研究结论的差异性,厘清"入遗效应"理论演变的逻辑和原因,并且通过案例阐释这些变化,为国内遗产地构建可持续发展的世界遗产模式建立良好的理论基础。 展开更多
关键词 世界 入遗效应 产保护 可持续发展
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论文化遗产的原真性 被引量:4
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作者 沈坚 《中国名城》 2010年第3期13-18,共6页
文化遗产的核心价值,在于其原真性。国际通行以及国内长期认同的关于文化遗产的管理制度和规则,都是围绕着严格保持其本来面目、反对造假这一点展开的。无论是申报世界遗产,还是在入遗后持续展开的维护工作中,都应始终坚守原真性这一基... 文化遗产的核心价值,在于其原真性。国际通行以及国内长期认同的关于文化遗产的管理制度和规则,都是围绕着严格保持其本来面目、反对造假这一点展开的。无论是申报世界遗产,还是在入遗后持续展开的维护工作中,都应始终坚守原真性这一基本原则。 展开更多
关键词 文化 原真性 入遗
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Production of Partial New-typed Brassica Napus by Introgression of Genomic Components from B. rapa and B. carinata 被引量:1
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作者 栗茂腾 刘建民 +2 位作者 王艳婷 余龙江 孟金陵 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第5期460-468,共9页
A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A^r subgenome of B. rapa (A^rA^r) and C^c of B. carinata (B^cB^cC^cC^c) into natural B. napus (AnAncncn). The... A breeding strategy for widening the germplasm of Brassica napus was proposed by introgression of the A^r subgenome of B. rapa (A^rA^r) and C^c of B. carinata (B^cB^cC^cC^c) into natural B. napus (AnAncncn). The progenies with 38 chromosomes that were derived from the self-pollinated seeds of pentaploid hybrids (A^rA^nB^cC^cC^n) were used for further research. Some of the partial new-typed B. napus showed normal meiotic behavior, high portion of germinated pollen and normal embryological development. This indicates that the selected new-typed B. napus had a balanced genetic base. Molecular analysis showed that about 50% of the genome in the new-typed B. napus was replaced by A^r and C^c subgenome from B. rapa and B. carinata. Considering the genetic diversity among different lines of new-typed B. napus, it was deduced that the introgression of the genomic components from B, rapa and B. carinata could widen the genetic diversity of rapeseed. 展开更多
关键词 new-typed B. napus pollen germination embryo sac development genome introgression genetic distance
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Tumor differentiation phenotype in gastric differentiated-type tumors and its relation to tumor invasion and genetic alterations 被引量:9
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作者 Kimiyasu Yamazaki Yusuke Tajima +7 位作者 Reiko Makino Nobukazu Nishino Shigeo Aoki Masanori Kato Masaaki Sakamoto Koji Morohara Tsutomu Kaetsu Mitsuo Kusano 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第24期3803-3809,共7页
AIM: To clarify the relations between tumor differentiation phenotype and tumor invasion or genetic alterations in gastric differentiated-type tumors. METHODS: We examined the tumor differentiation phenotype, the pr... AIM: To clarify the relations between tumor differentiation phenotype and tumor invasion or genetic alterations in gastric differentiated-type tumors. METHODS: We examined the tumor differentiation phenotype, the presence of mutations in APC and p53, and the microsatellite instability (MSI) status in 48 gastric adenomas and 171 differentiated-type carcinomas, The tumor differentiation phenotype was determined by examining the expression of human gastric mucin (HGM), NUC6, MUC2 and CD10, The tumors were then classified into gastric- (G-), gastric and intestinal mixed (GI-), or intestinal- (I-) phenotypes, according to the immunopositivity of the above markers, The presence of mutations in APC and p53 and the MSI status were also investigated in all the tumors, RESULTS: Gastric adenomas were significantly associated with CDIO expression, I-phenotype tumors and the presence of APC mutations, compared with carcinomas (66.7% vs 25.1%, P 〈 0.0001; 56.3% vs 14.6%, P 〈 0.0001; 39.6% vs 14.0%, P 〈 0.0001, respectively) and inversely associated with expressions of HGM and MUC6 and the presence of p53 mutations (10.4% vs 62.6%, P 〈 0.0001; 39.6% vs 64.3%, P = 0.003; 2.0% vs 26.3%, P = 0.001, respectively). The frequency of APC mutations was significantly higher in HGM-negative tumors, MUC6-negative tumors, CD10-positive tumors and I-phenotype tumors than in HGM-positive tumors, MUC6- positive tumors, CD10-negative tumors and G-phenotype tumors (32.7% vs 7.1%, P 〈 0.0001; 27.8% vs 14.0%, P = 0.0182; 37.3% vs 10.4%, P 〈 0.0001; and 38.5% vs 9.5%, P = 0.0017, respectively). The frequency of MSI was significantly higher in MUC6-positive tumors, CD10- negative tumors and G-phenotype tumors than in MUC6- negative tumors, CD10-positive tumors and I-phenotype tumors (24.8% vs 6.7%, P = 0.0009; 22.2% vs 8.0%, P = 0.0143; and 28.6% vs 9.6%, P = 0.0353, respectively). CONCLUSION: The tumor differentiation phenotype is closely related to tumor invasion and genetic alterations in gastric differentiated-type tumors. 展开更多
关键词 Gastric carcinoma Tumor differentiation phe-notype APC p53 Microsatellite instability
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Preimplantation genetic diagnosis for Down syndrome pregnancy 被引量:2
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作者 ZHANG Yu XU Chen-ming ZHU Yi-min DONG Min-yue QIAN Yu-li JIN Fan HUANG He-feng 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第7期515-521,共7页
Objective: To evaluate the effect of preimplantation genetic diagnosis (PGD) conducted for women who had Down syndrome pregnancy previously. Methods: Trisomy 21 was diagnosed by using fluorescence in site hybridizatio... Objective: To evaluate the effect of preimplantation genetic diagnosis (PGD) conducted for women who had Down syndrome pregnancy previously. Methods: Trisomy 21 was diagnosed by using fluorescence in site hybridization (FISH) before embryo transfer in two women who had Down syndrome pregnancies. Each received one or two PGD cycles respectively. Results: Case 1: one PGD cycle was conducted, two oocytes were fertilized and biopsied. One embryo is of trisomy 21 and the other of monosomy 21. No embryo was transferred. Case 2: two PGD cycles were conducted, in total, sixteen oocytes were fertilized and biopsied. Four embryos were tested to be normal, six of trisomy 21, and one of monosomy 21. Five had no signal. Four normal embryos were transferred but no pregnancy resulted. Conclusion: For couples who had pregnancies with Down syndrome pre-viously, PGD can be considered, and has been shown to be an effective strategy. 展开更多
关键词 Down syndrome Fluorescence in site hybridization (FISH) Preimplantation genetic diagnosis (PGD)
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Phenotype Observation and Variation Analysis of F_2 Generation of Ion Implanted Chili Pepper Seeds 被引量:1
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作者 汪宁 许峥 +4 位作者 张丰收 张涛 苏颖 连慕兰 周云龙 《Agricultural Science & Technology》 CAS 2011年第8期1103-1106,1132,共5页
[Objective]The aim was to investigate inheritance of the mutagenic properties caused by ion implantation from F1 to F2 generation in chili pepper.[Method]Chili pepper seeds were implanted with different ion combinatio... [Objective]The aim was to investigate inheritance of the mutagenic properties caused by ion implantation from F1 to F2 generation in chili pepper.[Method]Chili pepper seeds were implanted with different ion combinations at different doses,and the F1 generation seeds of five groups in which biological mutation occurred were selected to sow in the field.Then the main phenotype changes in F2 generation were observed,the biochemical changes caused by ion implantation were analyzed by determination of peroxidase isozyme.[Result]Seed implanted with 9×1011 P2+/cm2 and 1×1012 Cu2+/cm2(No.21)on its both sides could maintain the superiority in yield per plant to F2 generation,while the mutagenic effects of F1 generation in other groups were not inherited by the F2 generation.[Conclusion]The prominent biological characters induced in the seeds of group No.21 were relatively inherited,so the seeds were worth further breeding. 展开更多
关键词 Ion implantation Chili pepper Phenotype of F2 generation MUTATION HEREDITY
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An Optimization Design of a Weft Insertion Mechanism for Rapier Looms 被引量:5
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作者 竺志超 方志和 《Journal of Donghua University(English Edition)》 EI CAS 2003年第3期38-41,共4页
By analyzing a combined and spatial 6-bar linkage weft insertion mechanism, its practical model for optimization design is set up and the modification of penalty strategy is put forward so that the genetic algorithm c... By analyzing a combined and spatial 6-bar linkage weft insertion mechanism, its practical model for optimization design is set up and the modification of penalty strategy is put forward so that the genetic algorithm can be better used in optimization design for mechanisms with non- linear constraints. The design result is discussed. 展开更多
关键词 Combined mechanism weft insertion motion optimization design genetic algorithm
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Application of detecting algorithm based on network
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作者 张凤斌 杨永田 +1 位作者 江子扬 孙冰心 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2004年第3期275-280,共6页
Because currently intrusion detection systems cannot detect undefined intrusion behavior effectively, according to the robustness and adaptability of the genetic algorithms, this paper integrates the genetic algorithm... Because currently intrusion detection systems cannot detect undefined intrusion behavior effectively, according to the robustness and adaptability of the genetic algorithms, this paper integrates the genetic algorithms into an intrusion detection system, and a detection algorithm based on network traffic is proposed. This algorithm is a real-time and self-study algorithm and can detect undefined intrusion behaviors effectively. 展开更多
关键词 network security intrusion detection ANOMALY
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SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN
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作者 Fang-YinMeng Xiao-hongLi 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第4期285-285,共1页
关键词 Chromosome Aberrations Chromosomes Human Pair 21 Fertilization in Vitro Preimplantation Diagnosis ADULT Down Syndrome FEMALE Humans In Situ Hybridization Fluorescence Maternal Age PREGNANCY Pregnancy High-Risk Research Support Non-U.S. Gov't
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ORTHOGONAL DISCRETE FREQUENCY-CODING WAVEFORM DESIGN FOR MIMO RADAR 被引量:8
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作者 Liu Bo He Zishu 《Journal of Electronics(China)》 2008年第4期471-476,共6页
Design of orthogonal code sets with ideal correlation properties is crucial for orthogonalMultiple Input Multiple Output(MIMO)radar.A modified Genetic Algorithm(GA)is proposed tonumerically design orthogonal Discrete ... Design of orthogonal code sets with ideal correlation properties is crucial for orthogonalMultiple Input Multiple Output(MIMO)radar.A modified Genetic Algorithm(GA)is proposed tonumerically design orthogonal Discrete Frequency-Coding Waveforms(DFCWs)with good correlationproperties for MIMO radar.Some of the designed results are presented,and their correlation propertiesare better than those presented in literatures.The effect of Doppler frequency shift on the performanceof these signals is simply investigated.Simulation results and comparisons show that the proposedalgorithm is more effective for the design of DFCWs with superior aperiodic correlation properties. 展开更多
关键词 Multiple Input Multiple Output (MIMO) radar Netted radar Ambiguity function Genetic Algorithm (GA) Discrete Frequency-Coding Waveforms (DFCWs)
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Research on the Computer Network Protocol Test Model based on Genetic and Random Walk Algorithm
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作者 Ping Li 《International Journal of Technology Management》 2016年第8期39-42,共4页
In this paper, we conduct research on the computer network protocol test model based on genetic and random walk algorithm.Network protocol is the abstract concept, is important in the process of the development of net... In this paper, we conduct research on the computer network protocol test model based on genetic and random walk algorithm.Network protocol is the abstract concept, is important in the process of the development of network system. Fully understand and grasp of thenetwork protocols for managers is there is a big diffi cult. Network covert channel is the evaluation of intrusion detection system and fi rewallsecurity performance of an important means, the paper will start from the angle of the attacker, the fl aws of the research, and use this kind ofdefect to realize network covert channel, the random walk algorithm will be feasible for dealing with this issue. For achieving this, we integratethe genetic and random walk algorithm for systematic optimization. 展开更多
关键词 Computer Network Protocol Test Model Genetic and Random Walk Algorithm.
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PLC-Oriented Access Point Location Planning Algorithm in Smart-Grid Communication Networks 被引量:2
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作者 Ningzhe Xing Sidong Zhang +1 位作者 Yue Shi Shaoyong Guo 《China Communications》 SCIE CSCD 2016年第9期91-102,共12页
In this study, we investigate the optimal location of access points (APs) to connect end nodes with a service provider through power-line communication in smartgrid communication networks. APs are the gateways of po... In this study, we investigate the optimal location of access points (APs) to connect end nodes with a service provider through power-line communication in smartgrid communication networks. APs are the gateways of power-distribution communication networks, connecting users to control centers. Hence, they are vital for the reliable, safe, and economical operation of a power system. This paper proposes a planning method for AP allocation that takes into consideration economics, reliability, network delay, and (n-l) resilience. First, an optimization model for the AP location is established, which minimizes the cost of installing APs, while satisfying the reliability, network delay, and (n-1) resilience constraints. Then, an improved genetic algorithm is proposed to solve the optimization problem. The simulation results indicate that the proposed planning method can deal with diverse network conditions satisfactorily. Furthermore, it can be applied effectively with high flexibility and scalability. 展开更多
关键词 smart-grid communication network optimal location access point network delay genetic algorithm
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Hybrid Optimization of Support Vector Machine for Intrusion Detection
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作者 席福利 郁松年 +1 位作者 HAO Wei 《Journal of Donghua University(English Edition)》 EI CAS 2005年第3期51-56,共6页
Support vector machine (SVM) technique has recently become a research focus in intrusion detection field for its better generalization performance when given less priori knowledge than other soft-computing techniques.... Support vector machine (SVM) technique has recently become a research focus in intrusion detection field for its better generalization performance when given less priori knowledge than other soft-computing techniques. But the randomicity of parameter selection in its implement often prevents it achieving expected performance. By utilizing genetic algorithm (GA) to optimize the parameters in data preprocessing and the training model of SVM simultaneously, a hybrid optimization algorithm is proposed in the paper to address this problem. The experimental results demonstrate that it’s an effective method and can improve the performance of SVM-based intrusion detection system further. 展开更多
关键词 intrusion detection system IDS) support vector machine SVM) genetic algorithm GA system call trace ξα-estimator sequential minimal optimization(SMO)
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Nonlinear model predictive control based on support vector machine and genetic algorithm 被引量:5
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作者 冯凯 卢建刚 陈金水 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第12期2048-2052,共5页
This paper presents a nonlinear model predictive control(NMPC) approach based on support vector machine(SVM) and genetic algorithm(GA) for multiple-input multiple-output(MIMO) nonlinear systems.Individual SVM is used ... This paper presents a nonlinear model predictive control(NMPC) approach based on support vector machine(SVM) and genetic algorithm(GA) for multiple-input multiple-output(MIMO) nonlinear systems.Individual SVM is used to approximate each output of the controlled plant Then the model is used in MPC control scheme to predict the outputs of the controlled plant.The optimal control sequence is calculated using GA with elite preserve strategy.Simulation results of a typical MIMO nonlinear system show that this method has a good ability of set points tracking and disturbance rejection. 展开更多
关键词 Support vector machine Genetic algorithm Nonlinear model predictive control Neural network Modeling
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Institutional Distance and CrossNational Income Gaps
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作者 黄新飞 舒元 徐裕敏 《China Economist》 2014年第2期76-89,共14页
ihis paper examines the root causes of current cross-national institutional difference from the perspective of national hetereogeneity and provides detailed explanations on the justification and effectiveness of using... ihis paper examines the root causes of current cross-national institutional difference from the perspective of national hetereogeneity and provides detailed explanations on the justification and effectiveness of using cross-national genetic distance as the instrumental variable (IV) of institutional difference. We combined 10,585 samples by pairing 146 countries and regions, created a cross-national institutional distance variable composed of 14 indicators from the World Bank and the Heritage Foundation in three aspects including differences of political system, economic system and institutional implementation attributes, and conducted a cross-section IV estimation for the long-term effect of institutional differences on cross-national income gaps using data between 1996 and 2010. Empirical results indicate that institutional difference has a long-term significant positive effect on cross-national income gaps and such an effect has a tendency to increase during sample period. With factors like human capital, geographical factor, language and religion under control, we still arrived at similar conclusions. The empirical results are demonstrated to be robust using different genetic distance measurement indicators and estimation methods. 展开更多
关键词 institutional distance cross-national income gap genetic distance nationalheterogeneity estimation of instrumental variable
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The genetic relationship between Habo alkaline intrusion and its surrounding deposits, Yunnan Province, China: geological and S–Pb isotopic evidences 被引量:1
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作者 Zhongneng Meng Qian Zhang +3 位作者 Lin Ye Yupin Liu Jiangbo Lan Dapeng Wang 《Acta Geochimica》 EI CAS CSCD 2016年第4期391-407,共17页
The Habo alkaline intrusion, which is located in the south of the Sanjiang area, Yunnan Province, China, is a typical Cenozoic alkaline intrusion. There are a series of small to medium-sized Au and Pb-(Zn) deposits ... The Habo alkaline intrusion, which is located in the south of the Sanjiang area, Yunnan Province, China, is a typical Cenozoic alkaline intrusion. There are a series of small to medium-sized Au and Pb-(Zn) deposits around this intrusion. Those deposits are spatially associated with the Habo alkaline intrusion. (1) The δ^34S values of sulfides from Au deposits range from -1.91‰ to 2.69 ‰, which are similar to those of Pb-(Zn) deposits (-3.82 ‰ to -0.05 ‰) and both indicate a much greater contribution from magma. (2) The Habo alkaline intrusion has relatively homogeneous Pb isotopic compositions with ^206pb/^204pb ranging from 18.608 to 18.761, ^207pb/^204pb from 15.572 to 15.722 and ^20spb/^204pb from 38.599 to 39.110. These Pb isotope ratios are similar to those of Au deposits, whose ^206pb/^204pb range from 18.564 to 18.734, ^20Tpb/^20apb from 15.582 to 15.738 and ^208pb/^204pb from 38.592 to 39.319. Pb ratios in both the intrusion and Au deposits suggest that Pb mainly derived from the depth, probably represents a mixture of mantle and crust. Pb-(Zn) deposits, however, show a decentralized trait, and most of them are similar to that of the alkaline intrusion with ^206pb/^204pb ranging from 18.523 to 18.648, ^207pb/^204pb from 15.599 to 15.802, and ^20spb/^204pb from 38.659 to 39.206. (3) In the plumbotectonic diagram ^20Tpb/^204pb versus ^206pb/^204pb, almost all of Au and Pb-(Zn) deposits have the same projection area with the Habo alkaline intrusion, which indicates that those deposits almost share the same source with the alkaline intrusion. (4) Isotopic age of the Habo alkaline intrusion is 36-33 Ma, which is similar to that of Beiya, whose ore- related alkaline porphyries age is 38-31 Ma and molybdenite Re-Os age is 36.9 Ma. Therefore, along with S-Pb isotope traits, we suggest that the Habo Au and Pb-(Zn) deposits should be typically Ailaoshan-Red RiverCenozoicalkaline-related deposits and ore-forming ages of these deposits should be later than that of the Habo alkaline intrusion. 展开更多
关键词 The Habo Au and intrusion Ore genesis S-Pb materials Pb-(Zn) deposits - Alkaline isotope analyses - Source
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Low complexity user scheduling algorithms for uplink multiuser MIMO systems
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作者 李亮 邱玲 卫国 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2012年第2期105-112,共8页
Two efficient and low complexity multiuser scheduling algorithms are proposed for the uplink multi- ple-input multiple-output systems in this paper. Conventionally, the exhaustive search algorithm (ESA) can give the... Two efficient and low complexity multiuser scheduling algorithms are proposed for the uplink multi- ple-input multiple-output systems in this paper. Conventionally, the exhaustive search algorithm (ESA) can give the optimal performance; however, it is complexity prohibitive for practical implementation. Aiming at re- ducing the complexity while keeping the achievable sum rate performance, two heuristic algorithms are proposed for the multiuser scheduling problems: the improved genetic algorithm and simplified norm-based greedy algo- rithm. Moreover, we also consider the heterogeneity scenario where a modified grouping-based user selection al- gorithm is given to guarantee the user' s fairness. Specifically, the asymptotic behavior of the norm-based greed- y algorithm is given when each user is equipped with one antenna. Numerical examples demonstrate the superi- ority of our proposed schedulin~ and ~rouoin~ algorithms. 展开更多
关键词 Genetic algorithm (GA) multiuser scheduling muhiple-input multiple-output (MIMO)
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Nutritional Status and Associated Factors among Pregnant Women in Wondo Genet District, Southern Ethiopia
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作者 Desalegn Kuche Pragya Singh +1 位作者 Debebe Moges Tefera Belachew 《Journal of Food Science and Engineering》 2015年第2期85-94,共10页
Inadequate quality and quantity diet is one of the major reasons for high levels of malnutrition in pregnant women. A cross-sectional survey was conducted in Wondo Genet District, Southern Ethiopia. A two-stage cluste... Inadequate quality and quantity diet is one of the major reasons for high levels of malnutrition in pregnant women. A cross-sectional survey was conducted in Wondo Genet District, Southern Ethiopia. A two-stage cluster sampling technique was used to select a representative sample of 153 pregnant women aged 19-49 years from three rural villages ("Kebeles"). Energy and nutrient intakes from foods were calculated from one-day weighed food records on a sub-sample (n = 77). The result of the study showed that the intakes of most nutrients were lower than the recommended intake. The energy intake of the study participants both in 2nd and 3rd trimesters of pregnancy were 2,308 kcal and 1,420.5 kcal compared to the recommended 2,340 kcal and 2,452 kcal, respectively. Except iron, almost all micronutrient intakes were lower than the recommended intake. Vitamin A intake was 3/ag compared with the recommended 800μg, while protein intake of the study respondents in 2nd and 3rd trimester of pregnancy was 45.9 g and 31.5 g, respectively, compared with the recommended 71 g. Risk factors for undernutrition were multiple pregnancy and no consumption of cereal-based foods. This study revealed that the energy and nutrient intake of the pregnant women in study area was below the recommended intakes. Furthermore, the situation might be aggravated by high phytate content food consumption reported. Nutritional status of pregnant women in study area was not adequate to support the increased energy and nutrient requirement of the participants. 展开更多
关键词 Pregnant women nutritional status VITAMINS energy PROTEIN Southern Ethiopia.
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Preimplantation gender diagnosis by fluorescence in situ hybridization
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作者 徐艳文 庄广伦 +3 位作者 舒益民 李满 周灿权 张敏芳 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第6期874-877,152-153,共4页
Objective To describe the clinical application of fluorescence in situ hybridization (FISH) in preimplantation gender diagnosis.Methods Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers,... Objective To describe the clinical application of fluorescence in situ hybridization (FISH) in preimplantation gender diagnosis.Methods Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 male patients with Y chromosome abnormality. Embryo sex was identified by FISH in total of 6 treatment cycles.Results A total of 123 cumulus-oocytes were retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male embryos were transferred to 5 patients in 6 cycles. Three healthy babies were born. The diagnosis was confirmed by subsequent analysis of amniocytes and embryonic buds after embryo reduction.Conclusions FISH is an efficient and reliable technique for determining the sex of human preimplantation embryos. Selective abortion and births of affected children can be avoided by preimplantation gender diagnosis. 展开更多
关键词 BLASTOCYST In Situ Hybridization Fluorescence Sex Determination (Analysis) Adult AMNIOCENTESIS Biopsy Female Humans Male Pregnancy Research Support Non-U.S. Gov't
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