MAPK(促分裂原活化蛋白激酶,Mitogen-Activated Protein Kinase)信号转导途径在植物病原真菌的生长发育、有性生殖和致病性调节等方面占有重要作用,是一种普遍存在的细胞外信号转导途径。在真核生物MAPK蛋白的同源性和2种炭疽菌(禾谷炭...MAPK(促分裂原活化蛋白激酶,Mitogen-Activated Protein Kinase)信号转导途径在植物病原真菌的生长发育、有性生殖和致病性调节等方面占有重要作用,是一种普遍存在的细胞外信号转导途径。在真核生物MAPK蛋白的同源性和2种炭疽菌(禾谷炭疽、希金斯炭疽菌)全基因组数据库的基础上,结合Blastp、蛋白质结构域分析和聚类分析3种方法,从禾谷炭疽菌和希金斯炭疽菌基因组数据库中找出18个与酿酒酵母菌同源的3类MAPK级联信号途径基因,并绘制出这2种炭疽菌MAPK级联信号途径简图,为深入研究炭疽菌MAPK级联信号途径基因生物学功能及其相关信号网络特点奠定基础。展开更多
Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specifi...Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specificity of exome enrichment was approximately 80% with 95.6% even coverage of the 34 Mb target region at an average sequencing depth of 33-fold.Comparison of our results with whole genome shot-gun resequencing results showed that the exome SNP calls gave only 0.97% false positive and 6.27% false negative variants.Our protocol is also well suited for use with whole genome amplified DNA.The results presented here indicate that there is a promising future for large-scale population genomics and medical studies using a whole exome sequencing approach.展开更多
文摘MAPK(促分裂原活化蛋白激酶,Mitogen-Activated Protein Kinase)信号转导途径在植物病原真菌的生长发育、有性生殖和致病性调节等方面占有重要作用,是一种普遍存在的细胞外信号转导途径。在真核生物MAPK蛋白的同源性和2种炭疽菌(禾谷炭疽、希金斯炭疽菌)全基因组数据库的基础上,结合Blastp、蛋白质结构域分析和聚类分析3种方法,从禾谷炭疽菌和希金斯炭疽菌基因组数据库中找出18个与酿酒酵母菌同源的3类MAPK级联信号途径基因,并绘制出这2种炭疽菌MAPK级联信号途径简图,为深入研究炭疽菌MAPK级联信号途径基因生物学功能及其相关信号网络特点奠定基础。
基金supported by the Chinese Academy of Sciences (Grant Nos.GJHZ0701-6 and KSCX-YWN-023)the National Natural Science Foundation of China (Grant Nos.30725008,90403130,90608010,30221004,90612019 and 30392130)the National Basic Research Program of China (Grant Nos.2007CB815701,2007CB815703 and 2007CB815705)
文摘Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specificity of exome enrichment was approximately 80% with 95.6% even coverage of the 34 Mb target region at an average sequencing depth of 33-fold.Comparison of our results with whole genome shot-gun resequencing results showed that the exome SNP calls gave only 0.97% false positive and 6.27% false negative variants.Our protocol is also well suited for use with whole genome amplified DNA.The results presented here indicate that there is a promising future for large-scale population genomics and medical studies using a whole exome sequencing approach.
