目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,...目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术进行菌株鉴定和分子生物学研究,采用纸片扩散法(K-B法)检测其耐药表型,通过体外生物被膜形成实验检测其生物被膜形成能力,通过ParSNP构建亲缘关系图。结果MALDI-TOF MS可以准确鉴定猪红斑丹毒丝菌,该菌染色体大小为1791362 bp,鸟嘌呤(G)+胞嘧啶(C)含量为36.5%,含有1730个蛋白质编码序列,53个tRNA和2个rRNA,基因组分析暂未发现质粒;携带四环素类耐药基因tet(M),抗菌药物敏感性实验结果显示仅对四环素耐药,耐药基因和耐药表型一致;携带7个与黏附和荚膜形成相关的毒力基因;体外生物被膜形成能力适中(2+);与韩国分离株KC-Sb-R1亲缘关系较相近。结论本研究阐明了冷冻整鸡样本中猪红斑丹毒丝菌分离株的生物学特性,为该菌科学有效防控提供理论依据。展开更多
In recent years,with the rapid development of interdisciplinary studies such as molecular biology,genomics and systems biology,and with the breakthroughs in genome sequencing and other technologies,molecular marker-as...In recent years,with the rapid development of interdisciplinary studies such as molecular biology,genomics and systems biology,and with the breakthroughs in genome sequencing and other technologies,molecular marker-assisted selection and transgenic technology gradually became two important means of plant/animal breeding.Despite of the achievements,however,there are a number of problems in molecular breeding that need to be addressed immediately.As the major agronomic and economic traits of plants or animals are usually very complex and controlled by multiple genes,the modifi cation of a single or several functional genes is inadequate for the overall improvement of the target’s complex traits.Thus efficient,targeted展开更多
基金国家重点研发计划（2018YFD0100704）中国农业科学院科技创新工程专项经费项目（CAAS-ASTIP-2018-ZPRI）+2 种基金中国农业科学院基本科研业务经费（NO.1616032017209 and NO.1610192016209）现代农业产业技术体系建设专项（CARS-25-03）国家自然基金（31672178 and 31471893）
文摘目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术进行菌株鉴定和分子生物学研究,采用纸片扩散法(K-B法)检测其耐药表型,通过体外生物被膜形成实验检测其生物被膜形成能力,通过ParSNP构建亲缘关系图。结果MALDI-TOF MS可以准确鉴定猪红斑丹毒丝菌,该菌染色体大小为1791362 bp,鸟嘌呤(G)+胞嘧啶(C)含量为36.5%,含有1730个蛋白质编码序列,53个tRNA和2个rRNA,基因组分析暂未发现质粒;携带四环素类耐药基因tet(M),抗菌药物敏感性实验结果显示仅对四环素耐药,耐药基因和耐药表型一致;携带7个与黏附和荚膜形成相关的毒力基因;体外生物被膜形成能力适中(2+);与韩国分离株KC-Sb-R1亲缘关系较相近。结论本研究阐明了冷冻整鸡样本中猪红斑丹毒丝菌分离株的生物学特性,为该菌科学有效防控提供理论依据。
文摘In recent years,with the rapid development of interdisciplinary studies such as molecular biology,genomics and systems biology,and with the breakthroughs in genome sequencing and other technologies,molecular marker-assisted selection and transgenic technology gradually became two important means of plant/animal breeding.Despite of the achievements,however,there are a number of problems in molecular breeding that need to be addressed immediately.As the major agronomic and economic traits of plants or animals are usually very complex and controlled by multiple genes,the modifi cation of a single or several functional genes is inadequate for the overall improvement of the target’s complex traits.Thus efficient,targeted
