以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高...以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高,小鼠肠道菌群OTU数增加;与对照组相比,暴露组肠道菌群多样性下降,Alpha多样性指数Chao值、Shannon指数差异均显著(p<0.05)。在门水平上,拟杆菌门占比显著升高;在属水平上,普氏菌属比例变化明显,类杆菌属、乳酸杆菌属比例随着暴露剂量的提高而增加。研究结果初步揭示了PFHxS暴露造成与小鼠糖脂代谢相关的肠道菌群发生明显变化,为研究肠道菌群改变与机体糖脂代谢功能紊乱的相关性奠定基础。展开更多
目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛...目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛选探头超声功率、有机溶剂种类、卵磷脂与胆固醇及DSPE-PEG的投料比、PFH和Ce6用量、大豆油和e80磷脂用量,以及制备工艺;并对所制得纳米粒的粒度分布、Zeta电位、形态、包封率和载药量进行表征。同时应用流式细胞术研究细胞摄取情况。结果筛选出最优处方,成功制备了均一稳定的Ce6-PFH@PEGLip,其粒径为133.9±3.1 nm,PDI为0.211,Zeta电位为-39.1±2.3 m V,对Ce6的包封率为98.09%±1.6%。DSPEPEG的加入使得制剂更加稳定,提高了细胞对纳米粒的摄取效率。结论Ce6和PFH具有良好的相容性,能应用简单的处方和薄膜超声分散法制成共载长循环脂质体,在缺氧性肿瘤光动力治疗的应用具有广阔前景。展开更多
Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endo...Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1 st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope. Results: Perfluorodecalin droplets looking like “ fish eggs ” were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was sighificantly decreased (2789 ± 192 vs. 2341 ± 658, P < O. O1 ) and corneal thickness was increased. Conclusions: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction. Eye Sciemce 2001; 17:16 - 20.展开更多
Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group...Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group(F6H8) and control group(BSS) . All rabbits underwent anterior chamber injection of 0. 15ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopa-thological examination and Transmission electron microscopy(TEM) were done afterthe rabbits were sacrificed. Results : All the corneas were clear. Since 4 weeks after operation , the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM)grew gradually 2 weeks after surgely. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM. Conclusion: Corneal endothelial cell degenerated after contacting with F6H8 for 2 -4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade. Eye Science 2001: 17:21 - 26.展开更多
文摘以不同浓度的全氟己烷磺酸(PFHxS)分别灌胃暴露雄性Balb/c小鼠,连续暴露28 d后,收集小鼠的粪便,提取总DNA,并采用高通量测序技术对16S DNA V3-V4区进行测序。分析测序结果表明,28 d后最高剂量组小鼠体重显著性下降;随着PFHxS浓度的升高,小鼠肠道菌群OTU数增加;与对照组相比,暴露组肠道菌群多样性下降,Alpha多样性指数Chao值、Shannon指数差异均显著(p<0.05)。在门水平上,拟杆菌门占比显著升高;在属水平上,普氏菌属比例变化明显,类杆菌属、乳酸杆菌属比例随着暴露剂量的提高而增加。研究结果初步揭示了PFHxS暴露造成与小鼠糖脂代谢相关的肠道菌群发生明显变化,为研究肠道菌群改变与机体糖脂代谢功能紊乱的相关性奠定基础。
文摘目的制备共载光敏剂二氢卟吩(Ce6)和氧载体全氟己烷(PFH)的长循环脂质体,对其进行表征,并进行小鼠黑色素瘤细胞摄取的初步研究。方法采用薄膜超声分散法制备Ce6和PFH共载的PEG化长循环脂质体(Ce6-PFH@PEG-Lip),运用单因素筛选法依次筛选探头超声功率、有机溶剂种类、卵磷脂与胆固醇及DSPE-PEG的投料比、PFH和Ce6用量、大豆油和e80磷脂用量,以及制备工艺;并对所制得纳米粒的粒度分布、Zeta电位、形态、包封率和载药量进行表征。同时应用流式细胞术研究细胞摄取情况。结果筛选出最优处方,成功制备了均一稳定的Ce6-PFH@PEGLip,其粒径为133.9±3.1 nm,PDI为0.211,Zeta电位为-39.1±2.3 m V,对Ce6的包封率为98.09%±1.6%。DSPEPEG的加入使得制剂更加稳定,提高了细胞对纳米粒的摄取效率。结论Ce6和PFH具有良好的相容性,能应用简单的处方和薄膜超声分散法制成共载长循环脂质体,在缺氧性肿瘤光动力治疗的应用具有广阔前景。
基金This Work was Supported by the Scientific Fund Of the Ministrv of Health of China.
文摘Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1 st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope. Results: Perfluorodecalin droplets looking like “ fish eggs ” were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was sighificantly decreased (2789 ± 192 vs. 2341 ± 658, P < O. O1 ) and corneal thickness was increased. Conclusions: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction. Eye Sciemce 2001; 17:16 - 20.
文摘Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group(F6H8) and control group(BSS) . All rabbits underwent anterior chamber injection of 0. 15ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopa-thological examination and Transmission electron microscopy(TEM) were done afterthe rabbits were sacrificed. Results : All the corneas were clear. Since 4 weeks after operation , the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM)grew gradually 2 weeks after surgely. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM. Conclusion: Corneal endothelial cell degenerated after contacting with F6H8 for 2 -4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade. Eye Science 2001: 17:21 - 26.
