Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endo...Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1 st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope. Results: Perfluorodecalin droplets looking like “ fish eggs ” were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was sighificantly decreased (2789 ± 192 vs. 2341 ± 658, P < O. O1 ) and corneal thickness was increased. Conclusions: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction. Eye Sciemce 2001; 17:16 - 20.展开更多
Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group...Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group(F6H8) and control group(BSS) . All rabbits underwent anterior chamber injection of 0. 15ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopa-thological examination and Transmission electron microscopy(TEM) were done afterthe rabbits were sacrificed. Results : All the corneas were clear. Since 4 weeks after operation , the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM)grew gradually 2 weeks after surgely. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM. Conclusion: Corneal endothelial cell degenerated after contacting with F6H8 for 2 -4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade. Eye Science 2001: 17:21 - 26.展开更多
基金This Work was Supported by the Scientific Fund Of the Ministrv of Health of China.
文摘Purpose: To study the effects of perfluorodecalin on the cornea of the rabbit eyes. Methods: Perfluorodecalin (0.05 ml/each) was injected into the anterior chambers of eighteen rabbit eyes. Corneal morphology and endothelial cells were monitored clinically by slit-lamp biomicroscope and specular microscope for 26 weeks. Animals were sacrificed in 1 st, 2nd, 4th, 10th, 16th, 22nd, and 26th week after injection, respectively, and the corneas were examined under the light microscope. Results: Perfluorodecalin droplets looking like “ fish eggs ” were found at about 1/4-1/2 of the corneal height in the inferior anterior chamber. Corneal opacification on the area contacted with perfluorodecalin was observed in five eyes five weeks after injection, and all in the 22nd week. Mutton fat KPs in one eye were seen in the 6th week firstly, and in all eyes in the 7th week. Corneal pannus formation in one eye was present in the 4th week, two eyes in the 5th week and three eyes in the 6th week. Retrocorneal fibrous membrane in one eye was detected at the 6th week and 3 eyes at the 7th week respectively. After injection of perfluorodecalin, endothelial cell density was sighificantly decreased (2789 ± 192 vs. 2341 ± 658, P < O. O1 ) and corneal thickness was increased. Conclusions: Perfluorodecalin injected into anterior chamber can lead to corneal damage and inflammatory reaction. Eye Sciemce 2001; 17:16 - 20.
文摘Purpose: To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes. Methods: Fifteen New Zealand white rabbits were derided into two groups: experimental group(F6H8) and control group(BSS) . All rabbits underwent anterior chamber injection of 0. 15ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopa-thological examination and Transmission electron microscopy(TEM) were done afterthe rabbits were sacrificed. Results : All the corneas were clear. Since 4 weeks after operation , the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM)grew gradually 2 weeks after surgely. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM. Conclusion: Corneal endothelial cell degenerated after contacting with F6H8 for 2 -4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade. Eye Science 2001: 17:21 - 26.
