The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability harrier against the diffusion of substrates and products. Although common chemical or physical permeahiliza...The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability harrier against the diffusion of substrates and products. Although common chemical or physical permeahilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cell- bound glutamate decarboxylase （GAD） activity of recombinant Escherichia coil （BL21 （DE3）-pET28a-gadB） cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or suffactants to the culture media. Ampidllin was the most effective at improving cell-bound GAD activity of the BL21 （DE3）-pET28a-gadB, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 pg. ml-1. Using this concentration, the cell hiomass did decrease by 40.58%, but the cell-bound GAD activity of BL21 （DE3）-pET28a-gadB and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment ofBL21 （DE3）-pET28a-gadB cells with 5 tag.ml 1 ampicillin resulted in structural changes to the cell envelope, but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol （PVA）, alginate, and boric acid, the transfor- mation rate of γ-aminobutyric acid （GABA） at the 10th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after 30 days of storage at 4 ℃.展开更多
Objective To study the safety and effect of the umbilical cord blood （UCB）-derived mesenchymal stem cells （MSCs） on apoptosis of human cardiomyocytes （HCM）. Methods UCB was collected at the time of delivery with...Objective To study the safety and effect of the umbilical cord blood （UCB）-derived mesenchymal stem cells （MSCs） on apoptosis of human cardiomyocytes （HCM）. Methods UCB was collected at the time of delivery with informed consent obtained from 10 donors. The UCB-derived MSCs were treated with 5-azaserube （5-AZA） and were further induced to differentiate into cardiomyocytes. Telomerase activity, G-banding patterns of chromosomal karyotypes, tumor formation in nude mice, RT-PCR, and the effect of inhibiting apoptosis of HCM were investigated. Results MSCs derived from UCB were differentiated into cardiomyocytes in vitro, which possessed telomerase activity after 5-AZA induction, and no abnormal chromosomal karyotypes were observed. Expression of p53, cyclin A, cdk2, ~3 -actin, C-fos, h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment. There was no tumor formation in nude mice after injection of UCB-derived MSCs. UCB-derived MSCs significantly inhibited apoptosis of HCM. Conclusion UCB-derived MSCs are a valuable, safe and effective source of cell-transplantation treatment .展开更多
基金Supported by the grants from the National Natural Science Foundation of China(21176220,20876143,31470793)the Natural Science Foundation of Zhejiang Province(Z13B060008)the Key Technology Research and Development Project of Ningbo(2011C11023)
文摘The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability harrier against the diffusion of substrates and products. Although common chemical or physical permeahilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cell- bound glutamate decarboxylase （GAD） activity of recombinant Escherichia coil （BL21 （DE3）-pET28a-gadB） cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or suffactants to the culture media. Ampidllin was the most effective at improving cell-bound GAD activity of the BL21 （DE3）-pET28a-gadB, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 pg. ml-1. Using this concentration, the cell hiomass did decrease by 40.58%, but the cell-bound GAD activity of BL21 （DE3）-pET28a-gadB and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment ofBL21 （DE3）-pET28a-gadB cells with 5 tag.ml 1 ampicillin resulted in structural changes to the cell envelope, but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol （PVA）, alginate, and boric acid, the transfor- mation rate of γ-aminobutyric acid （GABA） at the 10th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after 30 days of storage at 4 ℃.
文摘Objective To study the safety and effect of the umbilical cord blood （UCB）-derived mesenchymal stem cells （MSCs） on apoptosis of human cardiomyocytes （HCM）. Methods UCB was collected at the time of delivery with informed consent obtained from 10 donors. The UCB-derived MSCs were treated with 5-azaserube （5-AZA） and were further induced to differentiate into cardiomyocytes. Telomerase activity, G-banding patterns of chromosomal karyotypes, tumor formation in nude mice, RT-PCR, and the effect of inhibiting apoptosis of HCM were investigated. Results MSCs derived from UCB were differentiated into cardiomyocytes in vitro, which possessed telomerase activity after 5-AZA induction, and no abnormal chromosomal karyotypes were observed. Expression of p53, cyclin A, cdk2, ~3 -actin, C-fos, h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment. There was no tumor formation in nude mice after injection of UCB-derived MSCs. UCB-derived MSCs significantly inhibited apoptosis of HCM. Conclusion UCB-derived MSCs are a valuable, safe and effective source of cell-transplantation treatment .
