Objective: To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods: Two crucial conditions (sodium chloride concentration and amount of the magnetic...Objective: To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods: Two crucial conditions (sodium chloride concentration and amount of the magnetic particles) were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction (PCR) were performed. Results: The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion: The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.展开更多
The rise of personalized flexible electronics has promoted the rapid development of flexible supercapacitors due to their long service life,fast charging-discharging rates and safe operation.Different from the traditi...The rise of personalized flexible electronics has promoted the rapid development of flexible supercapacitors due to their long service life,fast charging-discharging rates and safe operation.Different from the traditional flexible supercapacitors,the all-in-one integrated flexible supercapacitors are more resistant to deformation and lower interface resistance,which have a broader application prospect in the field of flexible electronics.This review briefly summarizes the preparation methods and electrochemical properties of some typical all-in-one supercapacitors,represented by planar and fibrous structures in recent years.Firstly,the basic understanding of traditional flexible supercapacitors and all-in-one integrated flexible supercapacitors,and the key parameters of the performance of flexible supercapacitors are introduced.Subsequently,the hydrogel matrix all-in-one supercapacitors with different functional characteristics(stretchable,self-healing and compressible)and the nonhydrogel matrix(separator,flexible membrane)all-in-one supercapacitors are discussed.Furthermore,the challenges and future development of flexible supercapacitors are considered.展开更多
We review the construction of gravitational solutions holographically dual to N=1 quiver gauge theories with dynamical flavor multiplets.We focus on the D3-D7 construction and consider the finite temperature,finite qu...We review the construction of gravitational solutions holographically dual to N=1 quiver gauge theories with dynamical flavor multiplets.We focus on the D3-D7 construction and consider the finite temperature,finite quark chemical potential case where there is a charged black hole in the dual solution.Discussed physical outputs of the model include its thermodynamics (with susceptibilities) and general hydrodynamic properties.展开更多
基金Supported by the National High Technology Research and Development Program of China (2006AA020705)
文摘Objective: To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods: Two crucial conditions (sodium chloride concentration and amount of the magnetic particles) were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction (PCR) were performed. Results: The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion: The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.
基金supported by the National Key Research and Development Program of China(2017YFB0406301)the State Key Laboratory of Electrical Insulation and Power Equipment(EIPE19210)+1 种基金the 111 Project of China(B14040)the Fundamental Research Funds for the Central University。
文摘The rise of personalized flexible electronics has promoted the rapid development of flexible supercapacitors due to their long service life,fast charging-discharging rates and safe operation.Different from the traditional flexible supercapacitors,the all-in-one integrated flexible supercapacitors are more resistant to deformation and lower interface resistance,which have a broader application prospect in the field of flexible electronics.This review briefly summarizes the preparation methods and electrochemical properties of some typical all-in-one supercapacitors,represented by planar and fibrous structures in recent years.Firstly,the basic understanding of traditional flexible supercapacitors and all-in-one integrated flexible supercapacitors,and the key parameters of the performance of flexible supercapacitors are introduced.Subsequently,the hydrogel matrix all-in-one supercapacitors with different functional characteristics(stretchable,self-healing and compressible)and the nonhydrogel matrix(separator,flexible membrane)all-in-one supercapacitors are discussed.Furthermore,the challenges and future development of flexible supercapacitors are considered.
基金supported by the European Community Seventh Frame work Programme FP7/2007-2013,under grant agreements n. 253534 and 253937. J.T.supported by the Netherlands Organization for Scientic Research (NWO) under the FOM Foundation research program+3 种基金supported by the MICINN and FEDER (grant FPA2008-01838)the Spanish Consolider-Ingenio 2010 Programme CPAN (CSD2007-00042)the Xunta de Galicia (Conselleria de Educacion and grant INCITE09-206-121-PR)KITPC Beijing for hospitality and financial support (this last under grant KJCX2.YW.W10 of the Chinese Academy of Sciences)
文摘We review the construction of gravitational solutions holographically dual to N=1 quiver gauge theories with dynamical flavor multiplets.We focus on the D3-D7 construction and consider the finite temperature,finite quark chemical potential case where there is a charged black hole in the dual solution.Discussed physical outputs of the model include its thermodynamics (with susceptibilities) and general hydrodynamic properties.