大豆分离蛋白-杏鲍菇多糖共价结合物对RAW264.7细胞的免疫调节作用

目的:研究大豆分离蛋白-杏鲍菇多糖共价结合物对小鼠巨噬细胞系RAW264.7的免疫调节作用。方法:将小鼠RAW264.7细胞分为空白组、脂多糖组和样品组(大豆分离蛋白、杏鲍菇多糖、大豆分离蛋白-杏鲍菇多糖混合物及共价结合物),分别测定并比较各组细胞的巨噬细胞活力、中性红吞噬活性、一氧化氮释放量、细胞因子分泌及其mRNA表达水平。结果:当质量浓度在0~50μg/mL范围时,大豆分离蛋白、杏鲍菇多糖、大豆分离蛋白-杏鲍菇多糖混合物和大豆分离蛋白-杏鲍菇多糖共价结合物组4种样品对细胞活力没有显著影响(P>0.05)。当大豆分离蛋白-杏鲍菇多糖共价结合物的质量浓度达到100μg/mL时,能够显著提高细胞的中性红吞噬活性、一氧化氮释放量以及肿瘤坏死因子-α、白细胞介素1β(interleukin 1β,IL-1β)和IL-6的含量及这3种细胞因子的mRNA表达水平(P<0.05),表明大豆分离蛋白-杏鲍菇多糖共价结合物能显著提升小鼠巨噬细胞系RAW264.7的免疫调节能力,且存在剂量依赖效应。结论:本研究可对大豆分离蛋白进行糖基化改性,为谷物蛋白的深加工和开发利用提供一定的理论依据。

培门冬酶治疗儿童急性淋巴细胞白血病致过敏性休克1例

培门冬酶(PEG-Asp)为新型门冬酰胺酶制剂,为聚乙二醇与门冬酰胺酶的共价结合物,具有免疫原性小,血浆半衰期长等优点,近年来逐渐成为急性淋巴细胞白血病(ALL)治疗的一线用药[1]。它无须皮试,用药次数少,患者易接受,但仍有极少数发生严重过敏反应。2016年2月,我科收治的1例ALL患儿在第二次VDLD方案化疗中发生过敏性休克,经积极抢救后好转。现将经过报道如下:

Synthesis and Characterization of a Two-dimensional Cadmium(II) Compound Involving Covalent and Hydrogen Bonds

A two-dimensional hydrogen-bonded cadmium(II) compound [Cd(dapm)2- (CH3COO)2(H2O)2] (dapm = diaminodiphenylmethane) has been synthesized and characterized by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group C2/c with a = 27.572(3), b = 5.5064(5), c = 23.310(2) , = 124.785(1)o, C15H19Cdo.5ON2O3, Mr =331.52, V = 2906.6(5) ?, Z = 4, Dc = 1.515 g/cm3, F(000) = 1368 and = 0.801 mm-1. The final R = 0.0403 and wR = 0.1014 for 1795 observed reflections with I≥4(I). The centrosymmetric Cd(II) is six- coordinated in a distorted octahedral geometry, and the dapm in a trans mode acts as a monoden- tate ligand. The intermolecular hydrogen bonds among coordinated aqua molecules with coordi- nated acetate oxygen atoms and uncoordinated dapm nitrogen atoms form a two-dimensional supramolecular framework.