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蛋白质分析技术的发展趋势 被引量:17
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作者 魏琴 吴丹 +2 位作者 张慧 李燕 李媛媛 《济南大学学报(自然科学版)》 CAS 2003年第4期312-320,共9页
在综述定量测定蛋白质的分析方法的基础上 ,着重阐述了染料、染料 -金属配合物作探针的吸光光度法、荧光光度法以及近几年来兴起的共振瑞利散射法在蛋白质的测定中的应用和发展动态 。
关键词 蛋白质 定量测定 吸光光度法 荧光光度法 共振瑞 利散射法
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True-color real-time imaging and spectroscopy of carbon nanotubes on substrates using enhanced Rayleigh scattering 被引量:1
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作者 Wenyun Wu Jingying Yue +21 位作者 Xiaoyang Lin Dongqi Li Fangqiang Zhu Xue Yin Jun Zhu Jiangtao Wang Jin Zhang Yuan Chen Xinhe Wang Tianyi Li Yujun He Xingcan Dai Peng Liu Yang wei Jiaping Wang Wei Zhang Yidong Huang Li Fan Lina Zhang Qunqing Li Shoushan Fan Kaili Jiang 《Nano Research》 SCIE EI CAS CSCD 2015年第8期2721-2732,共12页
Single-walled carbon nanotubes (SWCNTs) illuminated by white light should appear colored due to resonance Rayleigh scattering. However, true-color imaging of SWCNTs on substrates has not been reported, because of th... Single-walled carbon nanotubes (SWCNTs) illuminated by white light should appear colored due to resonance Rayleigh scattering. However, true-color imaging of SWCNTs on substrates has not been reported, because of the extremely low scattering intensity of SWCNTs and the strong substrate scattering. Here we show that Rayleigh scattering can be greatly enhanced by the interface dipole enhancement effect. Consequently colorful SWCNTs on substrates can be directly imaged under an optical microscope by wide field supercontinuum laser illumination, which facilitates high throughput chirality assignment of individual SWCNTs. This approach, termed "Rayleigh imaging microscopy", is not restricted to SWCNTs, but widely applicable to a variety of nanomaterials, which enables the colorful nanoworld to be explored under optical microscopes. 展开更多
关键词 enhanced Rayleigh scattering Rayleigh imaging microscopy true-color imaging carbon nanotube interface dipole
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Study on the interaction between palladium(II)-chlorpromazine hydrochloride and sodium tungstate by the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra and its analytical application 被引量:1
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作者 CHEN PeiLi LIU ShaoPu +2 位作者 LIU ZhongFang HU XiaoLi LI CuiXia 《Science China Chemistry》 SCIE EI CAS 2011年第3期506-514,共9页
The interaction between palladium(II)-chlorpromazine hydrochloride and sodium tungstate was investigated by ultravioletvisible absorption,resonance Rayleigh scattering(RRS),second-order scattering(SOS)and frequency do... The interaction between palladium(II)-chlorpromazine hydrochloride and sodium tungstate was investigated by ultravioletvisible absorption,resonance Rayleigh scattering(RRS),second-order scattering(SOS)and frequency doubling scattering (FDS)spectroscopy.In pH 5.3 Britton-Robinson(BR)buffer medium,chlorpromazine hydrochloride(CPZ)reacted with Pd(II) to form 2:1 cationic chelate,which further reacted with Na2WO4 to form a 1:1 ternary ion-association complex ([Pd(CPZ)2]·WO4).As a result,the signal intensities of RRS,SOS and FDS were enhanced greatly,and the enhancements of scattering were proportional to the CPZ concentration in a certain range.Their maximum wavelengths were located at 310 nm, 570 nm and 391 nm,respectively and the detection limits(3)were 1.6 ng/mL(RRS method),3.2 ng/mL(SOS method)and 5.6 ng/mL(FDS method).The optimum reaction conditions,the influences of coexisting substances and analytical application were mainly investigated by RRS method due to its highest sensitivity.A highly sensitive,simple,rapid and new method had been proposed to determine CPZ in the pharmaceutical form and residue of CPZ in pork.In addition,the Gibbs free energy change(△Gf)of ion-association reaction was computed by using B3LYP/3-21g*/LanL2dz method.The formation of ion-association and the reasons for the enhancement of RRS were also discussed. 展开更多
关键词 resonance Rayleigh scattering second-order scattering frequency doubling scattering chlorpromazine hydrochloride palladium(II) Na2WO4
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Temperature characteristics of near infrared SPR sen sors with Kretschmann configuration
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作者 田应鸿 郜洪云 +1 位作者 吴紫薇 黎敏 《Optoelectronics Letters》 EI 2015年第3期191-194,共4页
The temperature characteristics of near infrared surface plasmon resonance (SPR) sensors with Kretschmann configuration are studied theoretically and experimentally. The experimental results match with the numerical... The temperature characteristics of near infrared surface plasmon resonance (SPR) sensors with Kretschmann configuration are studied theoretically and experimentally. The experimental results match with the numerical simulations in the temperature range from 10 ℃ to 40.℃. With the increase of temperature, the resonance angle for gas increases slightly, but that for aqueous solution decreases obviously. No matter the dielectric layer is gas or aqueous solution, the resonance peaks are both broadened. 展开更多
关键词 Infrared devices RESONANCE SOLUTIONS
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