Objective To systematically evaluate the protection effect of electroacupuncture at ＂Neiguan＂（内关 PC 6） on myocardial damage by means of evidence-based medicine.Methods Databases were retrieved by computer,while ...Objective To systematically evaluate the protection effect of electroacupuncture at ＂Neiguan＂（内关 PC 6） on myocardial damage by means of evidence-based medicine.Methods Databases were retrieved by computer,while dissertation compilations and conference proceedings were searched manually.Data were analyzed statistically by using RevMan5.2software.Results Twelve studies were enrolled.Compared with myocardial ischemia reperfusion injury model group,① the magnitude of ECG STⅡ segment elevation can be reduced by means of electroacupuncture at PC 6（WMD=-73.95,95%CI[-109.69,-38.20]） and the difference was statistically significant（P0.0001）;② the ATPase in myocardial cells can be increased by means of electroacupuncture at PC 6（SMD=2.28,95%Cl[1.50,3.07]） and the difference was statistically significant（P0.00001）;③ the release of cTnT can be reduced by means of electroacupuncture at PC 6（WMD=-2.69,95%CI[-3.16,-2.22]） and the difference was statistically significant（P0.00001）;④ the size of myocardial infarction can be reduced by means of electroacupuncture at PC 6（WMD=-17.99,95%Cl[-19.88,-16.09]） and the difference was statistically significant（P0.00001）;⑤ the release of CK can be reduced by means of electroacupuncture at PC 6（WMD=-0.44,95%CI[-0.61,-0.27]） and the difference was statistically significant（P0.00001）;⑥ the release of CK-MB can be reduced by means of electroacupuncture at PC 6（SMD=-2.85,95%CI[-4.46,-1.24]） and the difference was statistically significant（P0.001）;⑦ the release of LDH can be reduced by means of electroacupuncture at PC 6（SMD=-2.97,95%CI[-4.87,-1.07]） and the difference was statistically significant（P0.01）.Conclusion Based on the current experimental evidences,electroacupuncture at PC 6 has a higher protective effect on myocardium.展开更多
Objective To explore protective effects of electroacupuncture at ＂Nèiguān＂ （内关 PC 6） for preconditioning on myocardial ischemia-reperfusion injury （MIRI） and the mechanisms involved. Methods Forty-eight ...Objective To explore protective effects of electroacupuncture at ＂Nèiguān＂ （内关 PC 6） for preconditioning on myocardial ischemia-reperfusion injury （MIRI） and the mechanisms involved. Methods Forty-eight male Wistar rats were randomly divided into a sham operation group （Group N）, a MIRI group （Group M） and an electroacupuncture （EA） group （Group E）. The MIRI model was established by ligating the left anterior descending artery （LAD） for 30 min followed by reperfusion for 120 min. Partition sutures were passed under LAD without ligation for rats in Group N. Rats in Group E were pretreated with electroacupuncture （EA） applied at bilateral ＂Nèiguān＂ （内关 PC 6） for 20 min once a day for 3 consecutive days before ischemia. The infarct size plus the area at risk was evaluated by 2,3,5-triphenyltetrazolium chloride staining, and serum isoenzyme of creatine kinase （CK-MB） and lactate dehydrogenase （LDH） levels were measured by biochemical methods. Myocardium morphological changes were observed under light microscopy. The mRNA expressions of myocardial δ and κ opioid receptors （DOR and KOR） were tested by real-time RT-PCR measurements. Results The myocardial infarct size in Group E was more significantly decreased than that in Group M （P0.05）. The levels of CK-MB [（980？±？92） U/L] and LDH [（2743？±？124） U/L] in Group M were significantly higher than those in Group N [（312？±？41） U/L] and [（530？±？56） U/L], respectively （both P0.01）. The levels of CK-MB [（572？±？70） U/L] and LDH [（1819？±？97） U/L] in Group E were significantly lower than those in Group M （both P0.01）. There were no significant differences in mRNA expressions of DOR and KOR between Group M and Group N （both P0.05）, but DOR expression in Group E was significantly higher than that either in Group M or in Group N （both P0.01 ）. No significant differences were found in KOR expression among the three groups （all P0.05）. Conclusion Up-regulation of expression of δ opioid receptors may be involved in protective effects of EA at Nèiguān （内关 PC 6） for preconditioning on MIRI.展开更多
基金Supported by Natural Science Foundation of China:81403460Hubei Provincial Education and Science"The 12th Five-Year Plan"Project:2014B095
文摘Objective To systematically evaluate the protection effect of electroacupuncture at ＂Neiguan＂（内关 PC 6） on myocardial damage by means of evidence-based medicine.Methods Databases were retrieved by computer,while dissertation compilations and conference proceedings were searched manually.Data were analyzed statistically by using RevMan5.2software.Results Twelve studies were enrolled.Compared with myocardial ischemia reperfusion injury model group,① the magnitude of ECG STⅡ segment elevation can be reduced by means of electroacupuncture at PC 6（WMD=-73.95,95%CI[-109.69,-38.20]） and the difference was statistically significant（P0.0001）;② the ATPase in myocardial cells can be increased by means of electroacupuncture at PC 6（SMD=2.28,95%Cl[1.50,3.07]） and the difference was statistically significant（P0.00001）;③ the release of cTnT can be reduced by means of electroacupuncture at PC 6（WMD=-2.69,95%CI[-3.16,-2.22]） and the difference was statistically significant（P0.00001）;④ the size of myocardial infarction can be reduced by means of electroacupuncture at PC 6（WMD=-17.99,95%Cl[-19.88,-16.09]） and the difference was statistically significant（P0.00001）;⑤ the release of CK can be reduced by means of electroacupuncture at PC 6（WMD=-0.44,95%CI[-0.61,-0.27]） and the difference was statistically significant（P0.00001）;⑥ the release of CK-MB can be reduced by means of electroacupuncture at PC 6（SMD=-2.85,95%CI[-4.46,-1.24]） and the difference was statistically significant（P0.001）;⑦ the release of LDH can be reduced by means of electroacupuncture at PC 6（SMD=-2.97,95%CI[-4.87,-1.07]） and the difference was statistically significant（P0.01）.Conclusion Based on the current experimental evidences,electroacupuncture at PC 6 has a higher protective effect on myocardium.
基金Supported by Guangdong TCM Bureau Project: 2008115
文摘Objective To explore protective effects of electroacupuncture at ＂Nèiguān＂ （内关 PC 6） for preconditioning on myocardial ischemia-reperfusion injury （MIRI） and the mechanisms involved. Methods Forty-eight male Wistar rats were randomly divided into a sham operation group （Group N）, a MIRI group （Group M） and an electroacupuncture （EA） group （Group E）. The MIRI model was established by ligating the left anterior descending artery （LAD） for 30 min followed by reperfusion for 120 min. Partition sutures were passed under LAD without ligation for rats in Group N. Rats in Group E were pretreated with electroacupuncture （EA） applied at bilateral ＂Nèiguān＂ （内关 PC 6） for 20 min once a day for 3 consecutive days before ischemia. The infarct size plus the area at risk was evaluated by 2,3,5-triphenyltetrazolium chloride staining, and serum isoenzyme of creatine kinase （CK-MB） and lactate dehydrogenase （LDH） levels were measured by biochemical methods. Myocardium morphological changes were observed under light microscopy. The mRNA expressions of myocardial δ and κ opioid receptors （DOR and KOR） were tested by real-time RT-PCR measurements. Results The myocardial infarct size in Group E was more significantly decreased than that in Group M （P0.05）. The levels of CK-MB [（980？±？92） U/L] and LDH [（2743？±？124） U/L] in Group M were significantly higher than those in Group N [（312？±？41） U/L] and [（530？±？56） U/L], respectively （both P0.01）. The levels of CK-MB [（572？±？70） U/L] and LDH [（1819？±？97） U/L] in Group E were significantly lower than those in Group M （both P0.01）. There were no significant differences in mRNA expressions of DOR and KOR between Group M and Group N （both P0.05）, but DOR expression in Group E was significantly higher than that either in Group M or in Group N （both P0.01 ）. No significant differences were found in KOR expression among the three groups （all P0.05）. Conclusion Up-regulation of expression of δ opioid receptors may be involved in protective effects of EA at Nèiguān （内关 PC 6） for preconditioning on MIRI.
