2015年7月,我国首个3D打印人体植入物——3D打印人工髋关节产品获得国家食品药品监督管理总局（China Food and Drug Administration,CFDA）注册批准。该产品也是国际上首个通过临床验证后获得注册的3D打印人工髋关节假体,标志着我国3D...2015年7月,我国首个3D打印人体植入物——3D打印人工髋关节产品获得国家食品药品监督管理总局（China Food and Drug Administration,CFDA）注册批准。该产品也是国际上首个通过临床验证后获得注册的3D打印人工髋关节假体,标志着我国3D打印植入物已迈进产品化阶段。本次获得CFDA注册的人工髋关节产品属于三类骨科植入物,是我国监管等级最高的医疗器械产品,是由北京大学第三医院骨科张克、刘忠军、蔡宏医生和国内最大的人工关节生产企业北京爱康宜诚医疗器材股份有限公司合作研制。展开更多
The study examined joint discontinuity spacing effect on drilling condition and performance in selected rocks in llorin and Ibese areas, Nigeria. Five samples for each rock type （gneiss and limestone） were tested in...The study examined joint discontinuity spacing effect on drilling condition and performance in selected rocks in llorin and Ibese areas, Nigeria. Five samples for each rock type （gneiss and limestone） were tested in the laboratory for chemical, physical and mechanical properties. Dip direction and joint spacing were measured using compass clinometers. The chemical composition was determined using X-ray Fluores- cence （XRF） spectrometer. The results show that gneiss has SiO2 of 61.88g and limestone has CaO content of 52.3g. The average dry density of gneiss and limestone are 2.6 and 2.39 g/cm^3, respectively. The uniaxial compressive strength of gneiss and limestone are 195 and 93.83 MPa, respectively. These rocks are classified as strong and moderately strong rock. Gneiss and limestone have mean joint discontinuity spacing of 0.79 and 0.25 m, which classified them as moderate and wide joint spacing respectively. Joint spacing was correlated with specific energy, bit wear and uniaxial compressive using Statistical Package for Social Science （SPSS）. The regression model has multiple coefficient of correlation of R^2 = 0.791 and R^2 =0.995 for gneiss and limestone, respectively. The variation in joint spacing could be attributed to spe- cific energy, bit wear and uniaxial compressive strength which affect drilling condition and performance. Ultimately, as joint spacing gets closer, the drilling velocity increases, drill string will be stable.展开更多
Objective To examine the expression of FLICE-inhibitory protein (FLIP) in juvenile idiopathic arthritis (JIA) and analyze its correlation with synovial inflammation. Methods The expression of FLIP was assessed in 11 J...Objective To examine the expression of FLICE-inhibitory protein (FLIP) in juvenile idiopathic arthritis (JIA) and analyze its correlation with synovial inflammation. Methods The expression of FLIP was assessed in 11 JIA and 3 normal synovial tissue samples by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The cell types expressing FLIP were further characterized,and the correlation of FLIP expression with the degree of synovial inflammation,as well as the activity of caspase 8 was then analyzed. Results RT-PCR revealed the expression of FLIP mRNA in all 11 JIA samples,but not in 3 normal synovial tissues. In JIA,FLIP expression could be found in both the lining and sublining layers,mainly in the macrophage-like cells. Moreover,the expression of FLIP in JIA synovial tissues was positively correlated with the degree of synovial inflammation (r=0.563,P<0.05). Conclusion The expression of antiapoptotic FLIP in JIA synovial tissue and its correlation to accumulation of inflammatory cells in synovial tissue suggests that FLIP potentially extends the lifespan of synovial cells and thus contributes to the progression of joint destruction.展开更多
Objective To study the effect and possible mechanism of Ligustrazine-a chinese traditional herbal medicine （CTM） on rat osteoarthritis （OA）. Methods OA model was surgically induced. Morphology of articular cartila...Objective To study the effect and possible mechanism of Ligustrazine-a chinese traditional herbal medicine （CTM） on rat osteoarthritis （OA）. Methods OA model was surgically induced. Morphology of articular cartilage was done by HE staining and mankin score was calculated; interleukin-1 and TNFα activity of rat joints was determined by 3-（4,5 dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） method; immunohistochemistry of MMP-13 and Cathepsin K was done by ABC method while the mRNA level for MMP-13, cathepsin K and tissue inhibitor of matrix metalloproteinase 1 （TIMP-1） was evaluated by RT-PCR method. ResultsLigustrazine （60 mg·kg^-1·d^-1 or 30 mg·kg^-1·d^-1 , but not 10 mg·kg^-1·d^-1 , im, ×8 weeks） reduced morphological changes of articular cartilage of OA rats. IL-1 and TNFα production of OA joints increases, together with MMP-13, cathepsine K and their mRNA levels in articular cartilage. The administration of Ligustrazine （60 mg·kg^-1·d^-1 -1 or 30 mg·kg^-1·d^-1 , im, ×8 weeks） reduced above changes significantly. At the same time, tissue inhibitor of metrix metalloprotease （TIMP1） mRNA level increased also in OA rats while Ligustrazine （60 mg·kg^-1·d^-1 , 30 mg·kg^-1·d^-1 or 10 mg·kg^-1·d^-1 , im, ×8 weeks） had no significant effect on it. Conclusion Ligustrazine （60 mg·kg^-1·d^-1 or 30 mg·kg^-1·d^-1 , but not 10 mg·kg^-1·d^-1 , im, ×8 weeks） effectively delays articular cartilage degradation of OA rats and could be a potential drug candidate for OA treatment.展开更多
Objective To study the MR characterizations of supraspinatus and infraspinatus tendon lesions by comparing with gross anatomy and histopathology. Methods The study group consisted of 20 cadaver shoulders which were un...Objective To study the MR characterizations of supraspinatus and infraspinatus tendon lesions by comparing with gross anatomy and histopathology. Methods The study group consisted of 20 cadaver shoulders which were underwent the same imaging protocols of conventional MRI and MR arthrography. Results SET2WI images or MR arthrography respectively possessed of high specificity （95. 6% , 100% ）, but low sensitivity （70. 6% , 58. 8% ） for diagnosing rotator cuff tears. By uniting two images techniques, could remedy its limitations and would markedly increase the sensitivity （88.2% ）. There were many factors influencing the visualizations of partial thickness tears of rotator cuff on MR images, from which uppermost factors are tear extent, ruptured synovial capsule, scar and synovial proliferation. Conclusion MR1 diagnoses of rotator cuff lesions （ especially partial thickness tears） must carefully be estimated by combining T2WI images and MR arthrography.展开更多
文摘2015年7月,我国首个3D打印人体植入物——3D打印人工髋关节产品获得国家食品药品监督管理总局（China Food and Drug Administration,CFDA）注册批准。该产品也是国际上首个通过临床验证后获得注册的3D打印人工髋关节假体,标志着我国3D打印植入物已迈进产品化阶段。本次获得CFDA注册的人工髋关节产品属于三类骨科植入物,是我国监管等级最高的医疗器械产品,是由北京大学第三医院骨科张克、刘忠军、蔡宏医生和国内最大的人工关节生产企业北京爱康宜诚医疗器材股份有限公司合作研制。
文摘The study examined joint discontinuity spacing effect on drilling condition and performance in selected rocks in llorin and Ibese areas, Nigeria. Five samples for each rock type （gneiss and limestone） were tested in the laboratory for chemical, physical and mechanical properties. Dip direction and joint spacing were measured using compass clinometers. The chemical composition was determined using X-ray Fluores- cence （XRF） spectrometer. The results show that gneiss has SiO2 of 61.88g and limestone has CaO content of 52.3g. The average dry density of gneiss and limestone are 2.6 and 2.39 g/cm^3, respectively. The uniaxial compressive strength of gneiss and limestone are 195 and 93.83 MPa, respectively. These rocks are classified as strong and moderately strong rock. Gneiss and limestone have mean joint discontinuity spacing of 0.79 and 0.25 m, which classified them as moderate and wide joint spacing respectively. Joint spacing was correlated with specific energy, bit wear and uniaxial compressive using Statistical Package for Social Science （SPSS）. The regression model has multiple coefficient of correlation of R^2 = 0.791 and R^2 =0.995 for gneiss and limestone, respectively. The variation in joint spacing could be attributed to spe- cific energy, bit wear and uniaxial compressive strength which affect drilling condition and performance. Ultimately, as joint spacing gets closer, the drilling velocity increases, drill string will be stable.
基金Supported by grants from the Chinese Ministry of Health (2008BAI59B01)the Department of Health of Sichuan province (020035)
文摘Objective To examine the expression of FLICE-inhibitory protein (FLIP) in juvenile idiopathic arthritis (JIA) and analyze its correlation with synovial inflammation. Methods The expression of FLIP was assessed in 11 JIA and 3 normal synovial tissue samples by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The cell types expressing FLIP were further characterized,and the correlation of FLIP expression with the degree of synovial inflammation,as well as the activity of caspase 8 was then analyzed. Results RT-PCR revealed the expression of FLIP mRNA in all 11 JIA samples,but not in 3 normal synovial tissues. In JIA,FLIP expression could be found in both the lining and sublining layers,mainly in the macrophage-like cells. Moreover,the expression of FLIP in JIA synovial tissues was positively correlated with the degree of synovial inflammation (r=0.563,P<0.05). Conclusion The expression of antiapoptotic FLIP in JIA synovial tissue and its correlation to accumulation of inflammatory cells in synovial tissue suggests that FLIP potentially extends the lifespan of synovial cells and thus contributes to the progression of joint destruction.
文摘Objective To study the effect and possible mechanism of Ligustrazine-a chinese traditional herbal medicine （CTM） on rat osteoarthritis （OA）. Methods OA model was surgically induced. Morphology of articular cartilage was done by HE staining and mankin score was calculated; interleukin-1 and TNFα activity of rat joints was determined by 3-（4,5 dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） method; immunohistochemistry of MMP-13 and Cathepsin K was done by ABC method while the mRNA level for MMP-13, cathepsin K and tissue inhibitor of matrix metalloproteinase 1 （TIMP-1） was evaluated by RT-PCR method. ResultsLigustrazine （60 mg·kg^-1·d^-1 or 30 mg·kg^-1·d^-1 , but not 10 mg·kg^-1·d^-1 , im, ×8 weeks） reduced morphological changes of articular cartilage of OA rats. IL-1 and TNFα production of OA joints increases, together with MMP-13, cathepsine K and their mRNA levels in articular cartilage. The administration of Ligustrazine （60 mg·kg^-1·d^-1 -1 or 30 mg·kg^-1·d^-1 , im, ×8 weeks） reduced above changes significantly. At the same time, tissue inhibitor of metrix metalloprotease （TIMP1） mRNA level increased also in OA rats while Ligustrazine （60 mg·kg^-1·d^-1 , 30 mg·kg^-1·d^-1 or 10 mg·kg^-1·d^-1 , im, ×8 weeks） had no significant effect on it. Conclusion Ligustrazine （60 mg·kg^-1·d^-1 or 30 mg·kg^-1·d^-1 , but not 10 mg·kg^-1·d^-1 , im, ×8 weeks） effectively delays articular cartilage degradation of OA rats and could be a potential drug candidate for OA treatment.
文摘Objective To study the MR characterizations of supraspinatus and infraspinatus tendon lesions by comparing with gross anatomy and histopathology. Methods The study group consisted of 20 cadaver shoulders which were underwent the same imaging protocols of conventional MRI and MR arthrography. Results SET2WI images or MR arthrography respectively possessed of high specificity （95. 6% , 100% ）, but low sensitivity （70. 6% , 58. 8% ） for diagnosing rotator cuff tears. By uniting two images techniques, could remedy its limitations and would markedly increase the sensitivity （88.2% ）. There were many factors influencing the visualizations of partial thickness tears of rotator cuff on MR images, from which uppermost factors are tear extent, ruptured synovial capsule, scar and synovial proliferation. Conclusion MR1 diagnoses of rotator cuff lesions （ especially partial thickness tears） must carefully be estimated by combining T2WI images and MR arthrography.
