[Objective] This study aimed to investigate the characteristics of deposition of inosine monophosphate (IMP) and intramuscular fat (IMF) in muscles of Jinghai yellow chickens and its crossbred.[Method] The charact...[Objective] This study aimed to investigate the characteristics of deposition of inosine monophosphate (IMP) and intramuscular fat (IMF) in muscles of Jinghai yellow chickens and its crossbred.[Method] The characteristics of IMP and IMF deposition of 112-day-old Jinghai yellow chickens (J×J) and its two different 70-day- old crossbreeds (J×B and B×B) were analyzed. The IMP content in breast muscle and leg muscle were determined by HPLC. [Result] The contents of IMP and cor- rected inosine monophosphate (IMPc) in breast muscle were significantly or ex- tremely significantly higher than that in leg muscle of the chickens in the three groups whether in male or female chickens (P〈0.05 or P〈0.01). There were no sig- nificant difference in the contents of IMP and IMPc between hens and roosters (P〉 0.05). The fresh degree of breast muscle and leg muscle was 96,11%-98.16% and 87.22%-93.07%, respectively. And the fresh degree of breast muscle was higher than that of leg muscle. In the three groups, the IMF content in leg muscle was significantly higher than that in breast muscle whether in male or female chickens (P〈0.05). The contents of IMF in breast muscle and leg muscle were 0.36%-0.75% and 1.84%-2.38%, respectively. The iMP content in breast muscle of chickens in Bx J group was extremely significantly higher than that in breast muscle of chickens in JxJ group (P〈0.01), but the contents of IMPc and iMF of breast muscle and leg muscle of the chickens in the three groups had no significant difference (P〉0.05). [Conclusion] To sum up, the freshness and flavor significantly differ between the breast muscle and leg muscle of chickens, but show no significant difference among the three groups.展开更多
A simplified Olami-Feder-Christensen model on a random network has been studied. We propose a new toppling rule -- when there is an unstable site toppling, the energy of the site is redistributed to its nearest neighb...A simplified Olami-Feder-Christensen model on a random network has been studied. We propose a new toppling rule -- when there is an unstable site toppling, the energy of the site is redistributed to its nearest neighbors randomly not averagely. The simulation results indicate that the model displays self-organized criticality when the system is conservative, and the avalanche size probability distribution of the system obeys finite size scaling. When the system is nonconservative, the model does not display scaling behavior. Simulation results of our model with different nearest neighbors q is also compared, which indicates that the spatial topology does not alter the critical behavior of the system.展开更多
In recent years, a number of organisations have implemented Executive Information Systems (EIS) including Computer-Based Information Systems (CBIS) in order to improve the performance of their executives' jobs th...In recent years, a number of organisations have implemented Executive Information Systems (EIS) including Computer-Based Information Systems (CBIS) in order to improve the performance of their executives' jobs though the majority of executives are unwilling to use EIS applications because of their design defects. Present researches on Executive Information Systems (EIS) are limited and less focused on the factors associated with EIS successful usage. This research adopts an extension of the Technology Acceptance Model (TAM) to derive useful variables to address the problem of the low usage of EIS by executives due to social factors, habits and facilitation conditions variables in Triandis' Framework. It reports on the adoption and usage of EIS by the executives in Organizations. The results show that experience in EIS usage was high with most respondents having used EIS for between 0 to 4 years (72.41%), followed by the 5-9 years group with 25.86%. However, in terms of ability to use EIS, most executives were at the expert (knowledgeable), casual user with 37.93%, followed by novice frequent user group with 31.03%. From this research, the authors propose a model that incorporates the habits, social factors and facilitating conditions in an Executive Information Systems.展开更多
Phosphodiesterase (PDE) exist various forms for the different structure and property, which is one of key components of light receptors in retinal rod cells. In this paper, cloning the DNA of T-subunits Phosphodiest...Phosphodiesterase (PDE) exist various forms for the different structure and property, which is one of key components of light receptors in retinal rod cells. In this paper, cloning the DNA of T-subunits Phosphodiesterase (γ-PDE6) from cDNA library, constructing the corresponding recombinant plasmid, and purification of the protein after initial expression was studied.展开更多
Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identi...Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine(HT) gene cluster in rice.This cluster contains a pyridoxamine 50-phosphate oxidase(Os PDX3) producing the cofactor pyridoxal50-phosphate(PLP), a PLP-dependent tyrosine decarboxylase(Os Ty DC1), and two duplicated hydroxycinnamoyl transferases(Os THT1 and Os THT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of Os Ty DC1 and Os THT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae(Xoo) and fungal pathogen Magnaporthe oryzae(M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens.展开更多
基金Supported by the Science and Technology Supporting Project (Agriculture) of Jiangsu Province (BE2011452)the Special Fund Project of the National Broiler Industry Technology System (CARS-42-G23)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)~~
文摘[Objective] This study aimed to investigate the characteristics of deposition of inosine monophosphate (IMP) and intramuscular fat (IMF) in muscles of Jinghai yellow chickens and its crossbred.[Method] The characteristics of IMP and IMF deposition of 112-day-old Jinghai yellow chickens (J×J) and its two different 70-day- old crossbreeds (J×B and B×B) were analyzed. The IMP content in breast muscle and leg muscle were determined by HPLC. [Result] The contents of IMP and cor- rected inosine monophosphate (IMPc) in breast muscle were significantly or ex- tremely significantly higher than that in leg muscle of the chickens in the three groups whether in male or female chickens (P〈0.05 or P〈0.01). There were no sig- nificant difference in the contents of IMP and IMPc between hens and roosters (P〉 0.05). The fresh degree of breast muscle and leg muscle was 96,11%-98.16% and 87.22%-93.07%, respectively. And the fresh degree of breast muscle was higher than that of leg muscle. In the three groups, the IMF content in leg muscle was significantly higher than that in breast muscle whether in male or female chickens (P〈0.05). The contents of IMF in breast muscle and leg muscle were 0.36%-0.75% and 1.84%-2.38%, respectively. The iMP content in breast muscle of chickens in Bx J group was extremely significantly higher than that in breast muscle of chickens in JxJ group (P〈0.01), but the contents of IMPc and iMF of breast muscle and leg muscle of the chickens in the three groups had no significant difference (P〉0.05). [Conclusion] To sum up, the freshness and flavor significantly differ between the breast muscle and leg muscle of chickens, but show no significant difference among the three groups.
文摘A simplified Olami-Feder-Christensen model on a random network has been studied. We propose a new toppling rule -- when there is an unstable site toppling, the energy of the site is redistributed to its nearest neighbors randomly not averagely. The simulation results indicate that the model displays self-organized criticality when the system is conservative, and the avalanche size probability distribution of the system obeys finite size scaling. When the system is nonconservative, the model does not display scaling behavior. Simulation results of our model with different nearest neighbors q is also compared, which indicates that the spatial topology does not alter the critical behavior of the system.
文摘In recent years, a number of organisations have implemented Executive Information Systems (EIS) including Computer-Based Information Systems (CBIS) in order to improve the performance of their executives' jobs though the majority of executives are unwilling to use EIS applications because of their design defects. Present researches on Executive Information Systems (EIS) are limited and less focused on the factors associated with EIS successful usage. This research adopts an extension of the Technology Acceptance Model (TAM) to derive useful variables to address the problem of the low usage of EIS by executives due to social factors, habits and facilitation conditions variables in Triandis' Framework. It reports on the adoption and usage of EIS by the executives in Organizations. The results show that experience in EIS usage was high with most respondents having used EIS for between 0 to 4 years (72.41%), followed by the 5-9 years group with 25.86%. However, in terms of ability to use EIS, most executives were at the expert (knowledgeable), casual user with 37.93%, followed by novice frequent user group with 31.03%. From this research, the authors propose a model that incorporates the habits, social factors and facilitating conditions in an Executive Information Systems.
文摘Phosphodiesterase (PDE) exist various forms for the different structure and property, which is one of key components of light receptors in retinal rod cells. In this paper, cloning the DNA of T-subunits Phosphodiesterase (γ-PDE6) from cDNA library, constructing the corresponding recombinant plasmid, and purification of the protein after initial expression was studied.
基金supported by the National Science Fund for Distinguished Young Scholars of China (31625021)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (31821005)+1 种基金the State Key Program of National Natural Science Foundation of China (31530052)the Hainan University Startup Fund (KYQD(ZR)1866)。
文摘Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine(HT) gene cluster in rice.This cluster contains a pyridoxamine 50-phosphate oxidase(Os PDX3) producing the cofactor pyridoxal50-phosphate(PLP), a PLP-dependent tyrosine decarboxylase(Os Ty DC1), and two duplicated hydroxycinnamoyl transferases(Os THT1 and Os THT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of Os Ty DC1 and Os THT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae(Xoo) and fungal pathogen Magnaporthe oryzae(M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens.