基于EJB3．0的煤矿分布式空间数据同步系统设计与实现

基于EJB3.0技术设计实现了煤矿分布式空间数据库同步系统,并结合LRGIS 3.0以地测部门的采掘工程平面图为例加以阐述,保障了煤矿空间数据的一致性、时效性,结合LR-GIS 3.0实现了矿山信息化管理。

GIS-Based Simulation of Engineering Construction Schedule

For its complexity, engineering construction schedule design is limited by various factors.Simulation-based engineering construction schedule takes critical path method (CPM) network as frame and calls complex cyclic operation network (CYCLONE) simulation model enclosed in advance for its simulation nodes. CYCLONE simulation model takes charge of simulating stochastic duration of activity and efficiency of resources, while CPM model performs project scheduling. This combination avoids the shortcomings of both models. Furthermore, geographic information system (GIS) technique is utilized to visualize the construction processes which are otherwise difficult to be understood by static results described. Application in practical project verifies the feasibility and advantage of the technique.

QUALITATIVE REASONING BY COMPUTING WITH WORDS IN HERRERA-MARTíNEZ’S LINGUISTIC MODEL

Most of modern systems for information retrieval, fusion and management have to deal with more and more qualitative information （by linguistic labels） besides information expressed quantitatively （by numbers）, since human reports are better and easier expressed in natural language than with numbers. In this paper, Herrera-Martfnez＇s 2-Tuple linguistic representation model is extended for reasoning with uncertain and qualitative information in Dezert-Smarandache Theory （DSmT） framework, in order to overcome the limitations of current approaches, i.e., the lack of precision in the final results of linguistic information fusion according to 1-Tuple representation （ q1 ）- The linguistic information which expresses the expert＇s qualitative beliefs is expressed by means of mixed 2 Tuples （equidistant linguistic labels with a numeric biased value）. Together with the 2-Tuple representation model, some basic operators are presented to carry out the fusion operation among qualitative information sources. At last, through simple example how 2-Tuple qualitative DSmT-based （q2 DSmT） fusion rules can be used for qualitative reasoning and fusion under uncertainty, which advantage is also showed by comparing with other methods.

Expression of CyclinD1／Bcl-1 and p27／Kip1 in Gliomas： Their Correlation with Pathological Grade and Prognosis

Objective: To study cyclinD1/bcl-1 and p27/kip1 expression in gliomas and their correlation with pathological grade and prognosis. Methods: Immunohistochemical technique was used to detect the cyclinD1/bcl-1 and p27/kip1 expression in 48 human brain glioma tissues of different malignant grades, and 12 normal non-neoplastic tissues collected from internal decompression. The data were analyzed quantitatively by the image system and also correlated retrospectively with the patients' clinical characteristics. Results: The immunohistochemical reaction for cyclinD1/bcl-1 and p27/kip1 was confined to the nuclei. The abnormal positive expression rates of both cyclinD1/bcl-1 and p27/kip1 in gliomas were found higher than that in non-neoplastic tissues(P<0.05). The number and staining intensity of cyclinD1/bcl-1 positive nuclei increased with malignant grades (P<0.05). On the contrary, the positive nuclei of p27/kip1 expression decreased in number and staining intensity with malignant grades(P<0.05). Higher expression of cyclinD1/bcl-1 or/and lower expression of p27/kip1 were associated with poor prognosis(P<0.05). Conclusion: The abnormal expression of both cyclinD1/bcl-1 and p27/kip1 might be closely related to the occurrence and development of gliomas and they might have synergistic effect. These data suggest that both cyclinD1/bcl-1 expression and p27/kip1 expression can act as an independent prognostic factor.

Developing new SSR markers from ESTs of pea(Pisum sativum L.)

The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.

RegIV expression showing specificity to gastrointestinal tract and its potential role in diagnosing digestive tract neuroendocrine tumor

Regenerating gene IV （RegIV）, a member of the regenerating gene family discovered in 2001, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate, but the overall expression profile of RegIV has not been reported. To learn more about RegIV, we evaluated its distribution by immunohistochemistry （IHC） in a total of 360 samples including 24 types of normal tissue, 40 benign and malignant lesions, and 18 neuroendocrine tumors. We found that in normal tissues, in addition to its relative specificity for the gastrointestinal tract, RegIV was detected in the adrenal gland and mammary gland. Among all the malignancies of various histological types under evaluation, RegIV was found mostly in adenocarcinomas. Studies on additional sets of colorectal tumor samples showed that RegIV expression was predominant in colorectal adenoma （87.5%） and peritumoral tissue （100%） but not in cancer tissue （30.8%）. Among neuroendocrine tumors, ReglV had a relatively restricted expression to those of digestive system.

Development of solid-state nanopore fabrication technologies

As the key components of nanopore-based nucleic acid sequencing systems, nanopores have drawn more and more scientific interests over these years. Although most of the early nanopore-based sequencers adopted biological nanopores, solid-state nanopores have been gradually growing in popularity due to their increased robustness and durability, control over pore geometry and surface properties, as well as compatibility with the existing semiconductor and microfiuidics fabrication techniques. Besides acting as a platform for biomolecular analysis, solid-state nanopores also have great potential in many other fields such as near-field optics, nanostencil lithography and ionic logic circuitry, due to the possibility of parallel massive production. Therefore, many approaches for the fabrication of solid-state nanopores have been developed. This paper reviews the typical solid-state nanopore fabrication techniques reported to date and compares their advantages and disadvantages. The specific applications of each kind of solid-state nanopores are also summarized based on the carefully analysis of their unique morphologies and properties such as the feature size, inner structure and possibility of massive production.

A microarray analysis of early activated pathways in concanavalin A-induced hepatitis

Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and progression.Methods:Twelve BALB/c mice were divided into four groups:one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline(PBS),and another three groups with concanavalin A(Con A) administration sacrificed at 1,3,and 6 h after injection.Affymetrix GeneChip Mouse 430 2.0 Array was employed to evaluate the expression profile of each of the 12 samples.Further analysis was done on the microarray data to extract the genes that were differentially expressed.Enrichment analysis was carried out to determine relevant pathways within which regulated genes were significantly enriched.Results:A total of 393,8354 and 11 344 differentially expressed genes were found,respectively,at three time points.During 0-1 h and 1-3 h,most of the pathways enriched with regulated genes were related to immune response and inflammation,among which Toll-like receptor(TLR) signaling and mitogen-activated protein kinase(MAPK) signaling appeared during both phases,while cytokine-cytokine receptor interaction,apoptosis,T cell receptor signaling,and natural killer(NK) cell-mediated cytotoxicity pathways emerged during the second phase.Pathways found to be significant during 3-6 h were mostly related to metabolic processes.Conclusion:The TLR signaling pathway dominates the early responses of Con A-induced FH in mice.It stimulates the production of type I cytokines,therefore recruiting and activating T/NK cells.Activated T/NK cells exert their cytotoxicity on hepatocytes through inducing death receptorintermediated apoptosis,resulting in liver injury.