关门穴与治疗部位神经相关性解剖学研究

目的探索分布关门穴的神经与针刺关门穴治疗部位(胃)的神经是否存在着关联。方法选择经过福尔马林固定的尸体5具(男性尸体4具,女性尸体1具),采用钢针刺到关门穴然后固定,再进行层次解剖尸体,同时解剖分离胃周围的神经。结果关门穴下为皮肤,皮下组织,腹直肌鞘前层,腹直肌,腹直肌鞘后层,腹横筋膜,腹膜下筋膜。关门穴治疗部位神经追溯至胃迷走神经,继续寻找神经支路发现交感神经节纤维增多,神经随着脾血管经脾门进入脾内。结论关门穴治疗部位神经与脾胃的神经存在着相关联系,为针灸治疗很有意义。

隔药灸治疗脾胃气虚型糖尿病胃轻瘫的疗效观察

目的观察隔药灸三门穴(梁门、关门、滑肉门)治疗脾胃气虚型糖尿病胃轻瘫的临床疗效。方法将126例脾胃气虚型糖尿病胃轻瘫患者随机分为治疗组、对照1组和对照2组,每组42例。治疗组给予隔药灸“三门穴”治疗,对照1组给予隔药灸常规腧穴(足三里、天枢、中脘)治疗,对照2组采用口服枸橼酸莫沙必利片治疗。治疗6周后观察各组胃轻瘫主要症状指数量表(GCSI)各项评分(早饱、胃胀、恶心呕吐、食欲不振)及各项中医症状(脘腹胀满、食欲不振、疲乏无力、恶心呕吐)评分的变化情况,并比较各组治疗前后4h胃排空率及临床疗效。结果3组治疗后GCSI各项评分、各项中医症状评分及治疗后4h胃排空率与同组治疗前比较,差异均具有统计学意义(P<0.05)。治疗组治疗后GCSI各项评分、各项中医症状评分及治疗后4h胃排空率与对照1组和对照2组比较,差异均具有统计学意义(P<0.05)。治疗组总有效率为92.9%,对照1组为81.0%,对照2组为66.7%,治疗组总有效率与对照1组和对照2组比较,差异具有统计学意义(均P<0.05)。结论隔药灸“三门穴”能明显改善脾胃气虚型糖尿病胃轻瘫患者的临床症状及胃排空率,临床疗效显著。

针刺加闪罐治疗老年2型糖尿病胃轻瘫临床观察

目的观察针刺加闪罐治疗老年2型糖尿病胃轻瘫患者的临床疗效。方法 104例符合纳入标准的患者作为研究对象,随机分为治疗组和对照组,每组52例。对照组口服多潘立酮片,治疗组在对照组基础上加用针刺、闪罐治疗。8周后观察恶心/呕吐、反酸/嗳气、早饱、上腹胀痛症状改善情况,胃排空率及临床疗效差异。结果治疗组临床疗效明显高于对照组(P<0.05)。治疗组治疗后恶心/呕吐、反酸/嗳气、早饱、上腹胀痛评分明显低于对照组(P<0.05)。两组治疗后4 h胃排空率比较差异具有统计学意义(P<0.05)。结论针刺加闪罐能提高糖尿病胃轻瘫患者的胃排空率,有效改善临床症状,提高临床疗效。

Functional Feature of“Five Yang Acupoints”in Governor Vessel

Governor Vessel is one of eight extraordinary Meridians, and been also called ＂the sea of Yang Meridians＂. It masters the Yin and Yang of the body cooperating with Conception Vessel. Acupoints on Governor Vessel have been used widely in clinic. The author expounded the functional feature of Yaoyangguan （GV 3）, Mingmen （GV 4）, Zhiyang （GV 9）, Dazhui （GV 14） and Baihui （GV 20）, which can show the characteristic of Governor Vessel and provide reference for clinical application.

Effects of acupuncture on neuro-electrophysiological activities in hippocampal CAI and CA3 areas of rats with post-traumatic stress disorder

Objective: To observe the effects of acupuncture on the characteristics of neuro-electrophysiological activity in hippocampal CAI and CA3 areas of rats with post-traumatic stress disorder (PTSD). Methods: Fifty Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, a grasping group, a Western medicine group and an acupuncture group, with 10 rats in each group. Except for the blank group, rats in the other 4 groups all received the combined stress modeling method. Rats in the Western medicine group were intragastrically administrated with paroxetine hydrochloride, those in the acupuncture group received acupuncture intervention, those in the grasping group received grasping fixation, and those in the model group and the blank group did not receive any in terve ntions. After 14 d of in terve ntion, the interspike in terval (ISI) and power spectral den sities (PSD) were an a lyzed and mapped by in vivo multiple channels to record the neuron clusters discharge in the hippocampal CAI and CA3 areas. Results: Compared with the blank group, ISI was proIonged in the CAI and CA3 areas of the model group and the grasping group, and the concentrated PSD distribution area moved down (Pv0.05 or P<0.01). Compared with the grasping group, the ISI of the CAI and CA3 areas in the Western medicine group and the acupuncture group was shortened, and the concentrated PSD distribution area moved up (P<0.05 or P<0.01). The ISI and PSD distributions in the CAI and CA3 areas of the acupuncture group were not statistically different from those in the Western medicine group (both P>0.05). Conclusion: Both acupuncture and paroxetine hydrochloride can significantly regulate the neuro-electrophysiology activity of hippocampal CAI and CA3 areas in PTSD rats, which may be one of the mechanisms of acupuncture intervention to promote PTSD recovery.

Effect of acupuncture on blood oxygen concentration in brain of rats with post-traumatic stress disorder based on functional near-infrared spectroscopy

Objective: To observe the effect of acupuncture on blood oxygen concentration in the brain of rats with post-traumatic stress disorder (PTSD) based on functional near-infrared spectroscopy (fNIRS), thus to reveal the mechanisms of acupuncture in intervening the brain function of PTSD rats. Methods: Sixty Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, a grasping group, a paroxetine group and an acupuncture group, with 12 rats in each group. Except the blank group, rats in the other groups all received incarceration plus electric shock for 7 d to prepare the PTSD animal model. One hour before the stress model was established, rats in each group received the desig nated in tervention: rats in the blank group and the model group did not receive any intervention;rats in the grasping group received grasping and fixation;rats in the paroxetine group received paroxetine hydrochloride solution by intragastric administration;and rats in the acupuncture group received acupuncture. Six-day treatme nt was a course, with 2 courses of treatme nt con ducted for a total of 12 d. After the modeli ng, rats in each treatment group received intervention for 5 dz and the fNIRS system was used to collect and record the changes in the concentrations of oxygenated hemoglobin (HbO2), deoxygenated hemoglobin (d-Hb) and total hemoglobin (t-Hb) of the involved rat's brain regions, and also to assess the brain function. Results: Compared with the blank group, the con centrati on of HbO2 was significa ntly in creased, the concen trati on of d-Hb was significa ntly decreased, and the concen tration of t-Hb was sign ifica ntly in creased in the model group and the graspi ng group after the in tervention, and the differ en ces were statistically significant (all P<0.01). Compared with the model group, the concentrations of HbO2, d-Hb and t-Hb in the grasping group did not change significantly (all P>0.05). Compared with the grasping group, the concentration of HbC^was significantly decreased, the concentration of d-Hb was significantly in creased, and the concen trati on of t-Hb was sign ifica ntly decreased in the paroxeti ne group and the acup un cture group, and the differences were statistically sigrdficant (all P<0.05). There were no significant differences in the concentrations of HbO2, d-Hb and t-Hb between the paroxetine group and the acupuncture group (all P>0.05). Con elusion: Acup un cture can regulate the blood oxyge n conce ntrati on in the brain of PTSD model rats, which may be an important mechanism of acupuncture in intervening the brain function in PTSD rats.

Effect of liver-soothing and mind-regulating acupuncture on resting-state electroencephalographic signals in rats with post-traumatic stress disorder

Objective:To observe the effect of liver-soothing and mind-regulating acupuncture method on the resting-state electroencephalography (EEG) in rats with post-traumatic stress disorder (PTSD),and to provide evidence for the effect mechanism study and clinical application of acupuncture intervention for PTSD.Methods:Sixty Sprague-Dawley (SD) rats were randomly divided into a blank control group,a model group,a grasping group,a paroxetine group and an acupuncture group,with 12 rats in each group.Except for rats in the blank control group,rats in the other groups were subjected to preparing the PTSD models using 'incarceration plus electric shock' method.After interventions,changes in rat behavior of each group were observed;changes in resting-state EEG were collected and analyzed with multichannel EEG acquisition and analysis system,and image analysis and statistical processing were performed.Results:Compared with the blank control group,the average escape latency in the model group was significantly longer (P＜0.05),and the times of crossing the platform and the effective areas were all significantly reduced (P＜0.01).Compared with the grasping group,the average escape latencies in the paroxetine group and acupuncture group were significantly shortened (P＜0.05),and the times of crossing the platform and the effective areas were all significantly increased (P＜0.05).There were no significant differences in the average escape latency,the times of crossing the platform and the effective areas between the acupuncture group and paroxetine group (all P＞0.05).Compared with the blank control group,the α-wave power spectrum value in the model group was significantly decreased,and the power spectrum values of β-wave,δ-wave and a-wave were significantly increased (all P＜0.01);compared with the grasping group,α-wave power spectrum values in the paroxetine group and acupuncture group were significantly increased (both P＜0.01),and the power spectrum values of β-wave,δ-wave and a-wave were decreased significantly (all P＜0.01).The power spectrum values of α-wave,β-wave,δ-wave and (e)-wave of rats in the acupuncture group were not significantly different from those in the paroxetine group (all P＞0.05).Conclusion:Liver-soothing and mind-regulating acupuncture method can significantly improve the abnormal EEG activity in PTSD rats,which may be one mechanism of liver-soothing and mind-regulating acupuncture method in effectively affecting the brain function in PTSD rats.

Study on the angle of needling Yamen (GV 15) in atlanto-axial dislocation patients

Objective: To compare the risk angle and safety angle of n eedli ng Ya me n (GV 15) betwee n the atla nto-axial dislocati on (AAD) patients and healthy subjects. Methods: A total of 177 AAD patients diagnosed and treated at the Center of Upper Cervical Vertebra of Beijing Chin a-Japa n Frie ndship Hospital betwee n January 2010 and January 2018 were in eluded in the AAD group. Ano ther 207 healthy subjects were included in the normal group. There were totally 191 males and 193 females. The MRI sean was performed for the cervical vertebrae to measure the risk angle and safety angle of acup un cture at Ya me n (GV 15) on the sagittal image. Results: In the AAD group, the risk angle was (13.14±3.99)° and the relative safety angle was (10.31±3.23)° for the perpendicular needling, while the oblique needling risk angle was (9.09±3.09)° for the male;the risk angle was (12.12±2.74)° and the relative safety angle was (10.56±2.09)° for the perpendicular needling, while the oblique needling risk angle was (9.70±2.95)° for the female. In the normal group, the risk angle was (7.89±1.59)° and the relative safety angle was (10.21±3.55)° for the perpendicular needling, while the oblique needling risk angle was (16.07±1.77)° for the male;the risk angle was (6.93±1.45)° and the relative safety angle was (10.70±2.94)° for the perpendicular needling, while the oblique needling risk angle was (14.89±2.18)° for the female. The perpendicular needling risk angles for the males and females in the AAD group were larger than those in the normal group, and the differences were statistically significant (both P<0.01);for the inner-group comparison, there was no sigrHficant difference in the perpendicular needling risk angle between the male and the female in the AAD group (P>0.05);however, the perpendicular needling risk angle for the male was larger than the female, and the differenee was statistically significant in the normal group (P<0.01). There were no sign讦icant differences in the relative safety angle for both the male and the female between the AAD group and the normal group (both P>0.05). For the inner-group comparison, there was no sign讦icant differenee in the relative safety angle between the male and the female (P>0.05). The oblique needling risk angles for both the males and females were smaller in the AAD group than those in the normal group, and the differences were statistically sign讦icant (both P<0.01);the oblique needling risk angle for the male was not significantly different from that for the female in the AAD group (P>0.05);in the normal group, the oblique needling risk angle for the male was larger than that for the female, and the differenee was statistically significant (P<0.01). Conclusion: Un der the AAD condition, the risk angle and safety angle of acup un cture at Ya me n (GV 15) cha nge significantly, perpe ndicular n eedli ng should be better if performed slightly lower tha n the horiz on tai di recti on, and the oblique needling should be safer across the occipital foramen toward the occipital bone.