The analgesic interaction between electroacupuncture and intrathecal (i. t. ) administration of the non-selective excitatory amino acid receptor antagonist kynurenic acid (KYN) was investigated in a rat model of carra...The analgesic interaction between electroacupuncture and intrathecal (i. t. ) administration of the non-selective excitatory amino acid receptor antagonist kynurenic acid (KYN) was investigated in a rat model of carrageenan inflammatory pain. The nociceptive threshold of the latency of paw withdraw (PWL) to a noxious thermal stimulation was measured. Electroacupuncture (EA) unilateral (ipsilateral to carrageenan-injected paw) 'Zu-San-Li (St 36) and 'Huantia' (GB30) noints (0.3 ms duration. 2 and 60 Hz alternatelv. 1-2-3 mA.展开更多
目的观察抗氧化反应元件(ARE)活化剂5,6-二氢环戊烯并1,2-二硫杂环戊烯-3-硫酮(CPDT)对肌萎缩侧索硬化(ALS)体外器官培养模型的影响。探讨激活 ARE 途径后,在氧化应激状态下对运动神经元的保护作用。方法取出生8 d 乳鼠的脊髓腰段组织...目的观察抗氧化反应元件(ARE)活化剂5,6-二氢环戊烯并1,2-二硫杂环戊烯-3-硫酮(CPDT)对肌萎缩侧索硬化(ALS)体外器官培养模型的影响。探讨激活 ARE 途径后,在氧化应激状态下对运动神经元的保护作用。方法取出生8 d 乳鼠的脊髓腰段组织切片做脊髓器官培养,在培养液中加入谷氨酸转运体抑制剂苏-羟天冬氨酸(THA),升高细胞间隙谷氨酸浓度,造成脊髓前角运动神经元特异性损伤,制备成 ALS 体外器官培养模型。在此模型的基础上,将不同浓度的 CPDT(分别为15、30 μmol/L)加入培养液中进行干预。用神经元的特异性免疫组织化学染色剂非磷酸化神经丝单克隆抗体(SMI-32)对脊髓前角运动神经元进行鉴定,电镜观察运动神经元超微结构。结果用不同浓度的 CPDT 提前干预组运动神经元数目(个/张脊髓片,15 μmol/L 组15.81±6.97,30 μmol/L 组16.25±6.74)均较 THA 模型组(5.31±5.76)增多(n=15,P<0.05),且神经元周围存在着丰富的突起。而 CPDT 非提前干预组运动神经元数目分别为6.00±6.01、6.42±4.88,与模型组的运动神经元数目及形态差异无统计学意义。电镜观察 CPDT 提前干预组运动神经元超微结构保存较完整,与正常对照组比较无明显差异。结论活化 ARE 途径可以在一定程度上保护 THA 引起的运动神经元损伤。展开更多
文摘The analgesic interaction between electroacupuncture and intrathecal (i. t. ) administration of the non-selective excitatory amino acid receptor antagonist kynurenic acid (KYN) was investigated in a rat model of carrageenan inflammatory pain. The nociceptive threshold of the latency of paw withdraw (PWL) to a noxious thermal stimulation was measured. Electroacupuncture (EA) unilateral (ipsilateral to carrageenan-injected paw) 'Zu-San-Li (St 36) and 'Huantia' (GB30) noints (0.3 ms duration. 2 and 60 Hz alternatelv. 1-2-3 mA.