Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were prac...Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.展开更多
Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental condit...Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental conditions, including different temperatures（15℃, 20℃, and 25℃）, sunlight intensities（100%, 50%, and 25% of full solar radiation） and types of UVR（UV-A and UV-B）. Then, we determined the lengths, maximal quantum yields（ Fv/Fm）, and survival rates of the embryos. The results showed that the embryos had the highest lengths, Fv/Fm values and survival percentages when cultured under a temperature of 20℃ and 25% sunlight intensity. The full level of solar radiation severely reduced the growth and survival ratios of the embryos. Hizikia embryos recovered within 2 h from the damage caused by short-term（30 min） high radiation（about 70% of full solar radiation） and UVR exposure. However, the viabilities of the embryos were significantly decreased after 4 h exposure to UVR. According to these results, we propose that culture conditions with indoor natural light and room temperature would be favorable for the growth, development and physiology of H izikia embryos.展开更多
Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,an...Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,and results in a high yield of young abalones after being applied.展开更多
At present, all aspects of China have entered the era of a rapid development, while the development speed of digital electronic technology is especially outstanding. This is a new way for China＇ s new personnel train...At present, all aspects of China have entered the era of a rapid development, while the development speed of digital electronic technology is especially outstanding. This is a new way for China＇ s new personnel training, and also proposes higher requirements on this type of personnel training institutions. In view of the demands of society and different majors on personnel, an enhancement to the standardized management of electronic laboratory operations becomes one of the important tasks for personnel training institutions. In this paper, how to practice the standardization management in an all-round way is discussed centered at college and university students＇ electronic laboratory.展开更多
Indigenous innovation focused on the development of technical standards has become the core of China's innovation policy,since China released the "Outline of the National Program for Longand Medium-Term Scien...Indigenous innovation focused on the development of technical standards has become the core of China's innovation policy,since China released the "Outline of the National Program for Longand Medium-Term Scientific and Technological Development (2006-2020)" in 2006.Meanwhile,standardization issues are greatly emphasized by high-tech zones which serve as the major carriers of national economic growth as well as innovation clusters in the context of fast-growing international standardization.By means of the effective guide of the government,the standardization of high-tech zones in China now presents a wide range of operating modes.Specifically,they can be divided predominantly into the government promotion mode,the alliance collaboration mode,the research-standard-industry linkage mode,and the talent training mode,in terms of the main body of work and influence factors.展开更多
The current status and development history of domestic and abroad research reactors (RRs) are mentioned.The representative RRs and their respective technology characteristics are introduced.The utilizations of China&#...The current status and development history of domestic and abroad research reactors (RRs) are mentioned.The representative RRs and their respective technology characteristics are introduced.The utilizations of China's RRs,mainly included as nuclear engineering technology,basic research applications of nuclear technology,teaching and personnel training,are explained.展开更多
In this paper, we conduct research on the trend of modern interior design education reform from the perspectives of aesthetics and traditional regression. Professional interior design technology is to adapt to the nee...In this paper, we conduct research on the trend of modern interior design education reform from the perspectives of aesthetics and traditional regression. Professional interior design technology is to adapt to the needs of economic development of practical talents in the central plains, with the spirit of the ＂seeking truth from facts＂ , pinpoint the target of profession fostering, established the reasonable personnel training mode, trying to explore suitable for the market need of professional personnel training mode and practical teaching methods, architecture art and design professional teaching system in higher vocational colleges. Interior design professional teaching reform goal, the guiding ideology, clear, clearer thinking pay attention to professional skills and professional quality education, skills, for body, quality as the soul. Our research starts from the basic analysis of aesthetics and traditional regression to propose the novel educational paradigm that is innovative.展开更多
It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students...It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students will not make a fundamental achievement. Because it is not only an academic training but also the examination management and the humanities cultivation have a close connection. The humanities cultivation which is based on the core of virtue requires a good campus environment. Therefore, we should adjust to the medical students＇ humanities quality training requirements, making the targeted reform and innovation of examination management.展开更多
it is a case study of Jiangyin vocational and technical college, based on the development of the industry and the position requirements; it can analyze the current inadequacy of training mode. Based on skills developm...it is a case study of Jiangyin vocational and technical college, based on the development of the industry and the position requirements; it can analyze the current inadequacy of training mode. Based on skills development, it can reinforce students＇ talents cultivation objective of sustainable development, explore new mode of professional personnel training in interior design in higher vocational education.展开更多
Higher Vocational Education is showing unprecedented good momentum of development, for a large number of vocational technical talents will be the training object of society. As the number of students in higher vocatio...Higher Vocational Education is showing unprecedented good momentum of development, for a large number of vocational technical talents will be the training object of society. As the number of students in higher vocational colleges expands rapidly, their quality education and ability training have become more important. In conjunction with my teaching practice to engage in political work in colleges, from analyzing the problems of students ＇ ideological and political education, I elaborate approaches to strengthen students＇ ideological and political education.展开更多
At present, China＇s population aging of unprecedented increase, empty older family question discussion and solve the problem of pension, is the urgent needs of the population aging in our country. Compared with the t...At present, China＇s population aging of unprecedented increase, empty older family question discussion and solve the problem of pension, is the urgent needs of the population aging in our country. Compared with the traditional family endowment, institution endowment and community endowment that occupy the home can guarantee the old man in his own enjoy the professional care services in the community, its unique advantage by relevant government departments, institutions and community in favor of people. At the same time, the rapid development of science and technology will we＇re in a new era of big data, set offa wave of intelligent city, the era of Internet ＋ city community home endowment also ushered in the new opportunities and challenges, ＂community O2O＂ pension mode is more and more clear, the Internet information technology will be in the development of urban community endowment that occupy the home is very imoortant.展开更多
Under the background of global economic development, Training to meet market demand, the excellent translation professionals is imperative, as the professional computer aided translation is a very important subject of...Under the background of global economic development, Training to meet market demand, the excellent translation professionals is imperative, as the professional computer aided translation is a very important subject of college professional translation, it should be combined with market demand. In this paper, we analyzed and proposed practical solutions to the importance of computer-aided translation programs and the problems and difficulties currently faced. To create student-centered teaching principles, the computer translation soRware is the basis, and combines with a wide range of resources to adapt to a full range of training qualified personnel translation market.展开更多
基金Supported by the National Natural Science Foundation of China(No.41776153)the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ02-1)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11020304)the Postdoctoral Application Research Program funded by Qingdao(No.2016189)
文摘Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
基金Supported by the Science and Technology Planning Project of Guangdong,China(No.2016A020222001)
文摘Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental conditions, including different temperatures（15℃, 20℃, and 25℃）, sunlight intensities（100%, 50%, and 25% of full solar radiation） and types of UVR（UV-A and UV-B）. Then, we determined the lengths, maximal quantum yields（ Fv/Fm）, and survival rates of the embryos. The results showed that the embryos had the highest lengths, Fv/Fm values and survival percentages when cultured under a temperature of 20℃ and 25% sunlight intensity. The full level of solar radiation severely reduced the growth and survival ratios of the embryos. Hizikia embryos recovered within 2 h from the damage caused by short-term（30 min） high radiation（about 70% of full solar radiation） and UVR exposure. However, the viabilities of the embryos were significantly decreased after 4 h exposure to UVR. According to these results, we propose that culture conditions with indoor natural light and room temperature would be favorable for the growth, development and physiology of H izikia embryos.
文摘Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,and results in a high yield of young abalones after being applied.
文摘At present, all aspects of China have entered the era of a rapid development, while the development speed of digital electronic technology is especially outstanding. This is a new way for China＇ s new personnel training, and also proposes higher requirements on this type of personnel training institutions. In view of the demands of society and different majors on personnel, an enhancement to the standardized management of electronic laboratory operations becomes one of the important tasks for personnel training institutions. In this paper, how to practice the standardization management in an all-round way is discussed centered at college and university students＇ electronic laboratory.
文摘Indigenous innovation focused on the development of technical standards has become the core of China's innovation policy,since China released the "Outline of the National Program for Longand Medium-Term Scientific and Technological Development (2006-2020)" in 2006.Meanwhile,standardization issues are greatly emphasized by high-tech zones which serve as the major carriers of national economic growth as well as innovation clusters in the context of fast-growing international standardization.By means of the effective guide of the government,the standardization of high-tech zones in China now presents a wide range of operating modes.Specifically,they can be divided predominantly into the government promotion mode,the alliance collaboration mode,the research-standard-industry linkage mode,and the talent training mode,in terms of the main body of work and influence factors.
文摘The current status and development history of domestic and abroad research reactors (RRs) are mentioned.The representative RRs and their respective technology characteristics are introduced.The utilizations of China's RRs,mainly included as nuclear engineering technology,basic research applications of nuclear technology,teaching and personnel training,are explained.
文摘In this paper, we conduct research on the trend of modern interior design education reform from the perspectives of aesthetics and traditional regression. Professional interior design technology is to adapt to the needs of economic development of practical talents in the central plains, with the spirit of the ＂seeking truth from facts＂ , pinpoint the target of profession fostering, established the reasonable personnel training mode, trying to explore suitable for the market need of professional personnel training mode and practical teaching methods, architecture art and design professional teaching system in higher vocational colleges. Interior design professional teaching reform goal, the guiding ideology, clear, clearer thinking pay attention to professional skills and professional quality education, skills, for body, quality as the soul. Our research starts from the basic analysis of aesthetics and traditional regression to propose the novel educational paradigm that is innovative.
文摘It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students will not make a fundamental achievement. Because it is not only an academic training but also the examination management and the humanities cultivation have a close connection. The humanities cultivation which is based on the core of virtue requires a good campus environment. Therefore, we should adjust to the medical students＇ humanities quality training requirements, making the targeted reform and innovation of examination management.
文摘it is a case study of Jiangyin vocational and technical college, based on the development of the industry and the position requirements; it can analyze the current inadequacy of training mode. Based on skills development, it can reinforce students＇ talents cultivation objective of sustainable development, explore new mode of professional personnel training in interior design in higher vocational education.
文摘Higher Vocational Education is showing unprecedented good momentum of development, for a large number of vocational technical talents will be the training object of society. As the number of students in higher vocational colleges expands rapidly, their quality education and ability training have become more important. In conjunction with my teaching practice to engage in political work in colleges, from analyzing the problems of students ＇ ideological and political education, I elaborate approaches to strengthen students＇ ideological and political education.
文摘At present, China＇s population aging of unprecedented increase, empty older family question discussion and solve the problem of pension, is the urgent needs of the population aging in our country. Compared with the traditional family endowment, institution endowment and community endowment that occupy the home can guarantee the old man in his own enjoy the professional care services in the community, its unique advantage by relevant government departments, institutions and community in favor of people. At the same time, the rapid development of science and technology will we＇re in a new era of big data, set offa wave of intelligent city, the era of Internet ＋ city community home endowment also ushered in the new opportunities and challenges, ＂community O2O＂ pension mode is more and more clear, the Internet information technology will be in the development of urban community endowment that occupy the home is very imoortant.
文摘Under the background of global economic development, Training to meet market demand, the excellent translation professionals is imperative, as the professional computer aided translation is a very important subject of college professional translation, it should be combined with market demand. In this paper, we analyzed and proposed practical solutions to the importance of computer-aided translation programs and the problems and difficulties currently faced. To create student-centered teaching principles, the computer translation soRware is the basis, and combines with a wide range of resources to adapt to a full range of training qualified personnel translation market.
