[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium(Yixing Lily) in tissue culture.[Method] The different parts of scale,...[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium(Yixing Lily) in tissue culture.[Method] The different parts of scale,leaf and root of Yixing Lily were cultured as explants on MS basic medium supplemented with different concentrations of plant growth regulators,so as to compare their capacity to differentiate and regenerate.[Result] The explants had different potential to differentiate(scale root leaf).The capacity of different scale parts to differentiate was the lower part middle partupper part;the capacity of different leaf parts to differentiate was the leaf base middle part leaf tip;the capacity of different root parts to differentiate was the root base root tip middle part.[Conclusion] Tissue culture could be well applied in propagation of Yixing Lily.展开更多
Objective:Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked disorders caused by mutations in the dystrophin gene. The majority of recognized mutations are copy number changes of i...Objective:Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked disorders caused by mutations in the dystrophin gene. The majority of recognized mutations are copy number changes of individual exons. The objective of the present study was to assess the multiplex ligation-dependent probe amplification (MLPA) effects of detection of gene mutations. Methods: Samples of 20 control males and 80 males and their mothers referred to our diagnostic facility on the clinical suspi- cion of DMD or BMD were tested by MLPA and multiplex PCR. Results : The mean DQs for all peak of 20 control male samples was 1.02 (range from 0.83 to 1.21) by MLPA. Deletions or duplications were iden- tified in 6 out of 31 families that had been previously tested as negative by multiplex PCR. One case of complex rearrangement involving a duplication of two regions: dupEX3-9 and dupEX 17-41 were found by MLPA. Conclusions: MLPA is a highly sensitive method and rapid alternative to multiplex PCR for detec- tion of DMD and BMD.展开更多
Cells of in vitro cultured epidermis explants of ectoderm isolated at early gastrula stage,showed only weak excitability or even non-excitable at 6V when examined electrophysiologically.If non-excitable explants were ...Cells of in vitro cultured epidermis explants of ectoderm isolated at early gastrula stage,showed only weak excitability or even non-excitable at 6V when examined electrophysiologically.If non-excitable explants were treated with 100 mM glucose,the action potential (AP) appeared and within 1 hr reached its maximum.At the same time,their stimulus threshold became lowered gradually.And,if the glucose was washed out,AP gradually disappeared.If explants were treated with glucose of different concentrations,the percentage of explants which displayed AP increased with the increase of glucose concentration.When explants with approximately the same original stimulus threshold were treated with glucose of different concentrations,the stimulus threshold became lowered more in the more concentrated solution.If explants with different original stimulus thresholds were treated with glucose of the same concentration,the lowering of stimulus threshold was more obvious in those with higher original stimulus threshold.Other energy supplying substances used showed similar effect.展开更多
Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alter...Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative: how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA (amino acids), HMB (hydroxy-methyle-butyrate), creatine and camitine. If the athlete is involved in strength and power sport (e.g. throwing events in track and field, olympic weightlifting etc.), the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic (and anticatabolic) effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition (good balanced nourishment), as well, with appropriate application and supplementation of all vitamins and essential minerals.展开更多
With the continuous development of the times, cultivating students' cross-cultural consciousness is becoming increasingly urgent. The development of new situation requires that college English teachers should also st...With the continuous development of the times, cultivating students' cross-cultural consciousness is becoming increasingly urgent. The development of new situation requires that college English teachers should also strengthen students' cultural content education and cultivate students' cross-cultural consciousness, in order to stimulate students' learning interest, optimize students' knowledge structure and improve students' social cultural ability while introducing language knowledge and conducting listening, speaking, reading and writing training for students. Then, how to cultivate students' cross-cultural consciousness in college English teaching? This paper takes it as the theme for analysis, hoping to improve college English teaching quality.展开更多
Vocational students generally English foundation is weak, not strong interest in learning how to guide students to learn to think independently, to stimulate students' curiosity, is a series of problems faced by Engl...Vocational students generally English foundation is weak, not strong interest in learning how to guide students to learn to think independently, to stimulate students' curiosity, is a series of problems faced by English teachers in vocational college. In this paper, the issues currently facing vocational English education was of, and the corresponding countermeasures. With the development of economic and trade globalization, increasingly strengthened international exchanges. Society is not only high-level personnel to graduate raised higher and higher English proficiency requirements, the ability to develop applications based English Education in Higher Colleges also put forward higher requirements. Vocalional Training Colleges for professional and technical personnel is an important part of our personnel training requirements for rapid economic development of professional and technical personnel in addition to high technical expertise, but also need to understand the professional literature information inquiry, learning and further education. Therefore, we must from the height of the times and the needs of international competition to recognize new opportunities and challenges of English teaching in higher vocational colleges are facing a variety of approaches and strive to improve our English teaching level.展开更多
Microbes and microbial carbonates in reef-flat and coral community dynamics and submarine geomorphologic features in reef crest and fore reef of Yongxing Island,the Xisha Islands,South China Sea,were studied by means ...Microbes and microbial carbonates in reef-flat and coral community dynamics and submarine geomorphologic features in reef crest and fore reef of Yongxing Island,the Xisha Islands,South China Sea,were studied by means of scuba diving,underwater investigation,and line intercept transect survey.Studies indicate a very high coral mortality with few living corals in the reef flat of Yongxing Island.Moreover,macro algae,sea grass and cyanobacteria are common in reef flat.Microbes and microbially induced carbonates occur in reef flat.Living corals grow mainly in the reef crest and fore reef,but are also declined dramatically.From coast to off shore,the southeast reef flat of Yongxing Island can be divided into beach,inner reef flat,outer reef flat,reef flat front(reef crest and fore reef),and fore-reef slope settings.Sedimentary facies include coast,reef flat,reef crest and fore reef,and fore-reef slope.Reefal carbonate sediments are composed of coral skeletons and framework,coral fragments,bioclasts,and lime mud.With the deterioration of environment and water quality,the coral communities tend to be distributed in the reef crest and fore reef with clean sea water,well circulation and moderate water energy.Reef flat is occupied mainly by the macro algae and Heliopora coerulea communities.The coverage statistics on the reef crest demonstrate that the coverage of Acropora cytherea is more than 28% and represents a dominant species with wave-resistant ecological type.Sedimentary characteristics and geomorphologic features are different between the southeast and northwest reef-flat fronts(reef crest and fore reef) of Yongxing Island.The former shows discontinuously tidal channels in outer reef flat and different dimensional and deep reef ponds in reef crest and fore reef,and the latter presents a typical spur-and-groove system.Microbes(cyanobacteria Lyngbya sp.) occur generally in the inner reef flat and reef ponds of reef crest with restricted water circulation.Widely algae growth indicates a eutrophic environment,and the common microbes on the coral surface in the reef flat and reef ponds also demonstrate eutrophication in seawater and deteriorated water quality.展开更多
基金Supported by Fund for Scientific Research in Yangtze University(CDKF2283)Program of Engineering Research Center of Wetland Agriculture in the Middle Reaches of the Yangtze River of Ministry of Education~~
文摘[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium(Yixing Lily) in tissue culture.[Method] The different parts of scale,leaf and root of Yixing Lily were cultured as explants on MS basic medium supplemented with different concentrations of plant growth regulators,so as to compare their capacity to differentiate and regenerate.[Result] The explants had different potential to differentiate(scale root leaf).The capacity of different scale parts to differentiate was the lower part middle partupper part;the capacity of different leaf parts to differentiate was the leaf base middle part leaf tip;the capacity of different root parts to differentiate was the root base root tip middle part.[Conclusion] Tissue culture could be well applied in propagation of Yixing Lily.
文摘Objective:Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked disorders caused by mutations in the dystrophin gene. The majority of recognized mutations are copy number changes of individual exons. The objective of the present study was to assess the multiplex ligation-dependent probe amplification (MLPA) effects of detection of gene mutations. Methods: Samples of 20 control males and 80 males and their mothers referred to our diagnostic facility on the clinical suspi- cion of DMD or BMD were tested by MLPA and multiplex PCR. Results : The mean DQs for all peak of 20 control male samples was 1.02 (range from 0.83 to 1.21) by MLPA. Deletions or duplications were iden- tified in 6 out of 31 families that had been previously tested as negative by multiplex PCR. One case of complex rearrangement involving a duplication of two regions: dupEX3-9 and dupEX 17-41 were found by MLPA. Conclusions: MLPA is a highly sensitive method and rapid alternative to multiplex PCR for detec- tion of DMD and BMD.
文摘Cells of in vitro cultured epidermis explants of ectoderm isolated at early gastrula stage,showed only weak excitability or even non-excitable at 6V when examined electrophysiologically.If non-excitable explants were treated with 100 mM glucose,the action potential (AP) appeared and within 1 hr reached its maximum.At the same time,their stimulus threshold became lowered gradually.And,if the glucose was washed out,AP gradually disappeared.If explants were treated with glucose of different concentrations,the percentage of explants which displayed AP increased with the increase of glucose concentration.When explants with approximately the same original stimulus threshold were treated with glucose of different concentrations,the stimulus threshold became lowered more in the more concentrated solution.If explants with different original stimulus thresholds were treated with glucose of the same concentration,the lowering of stimulus threshold was more obvious in those with higher original stimulus threshold.Other energy supplying substances used showed similar effect.
文摘Peformance enhancing drugs are widely used today in different sport fields, and therefore the successful anti-doping activity should be based not only on regular doping controls, but also it should show the real alternative: how to replace the forbidden doping substances with legal and effective supplements. The paper deals with application proposition of protein concentrates, free AA (amino acids), HMB (hydroxy-methyle-butyrate), creatine and camitine. If the athlete is involved in strength and power sport (e.g. throwing events in track and field, olympic weightlifting etc.), the explosive and maximum strength is of primary importance. For strength athletes, these mentioned substances are those legal preparates, supplements, which can help effectively in performance improvement of sport results. The main reason is that using protein concentrates, AAs, HMB, creatine and camitine, the anabolic (and anticatabolic) effect will enhance the protein biosynthesis of the organism, improve the aerob and anaerob capacity of the athletes, activate and stimulate the own hormonal system. And the final result is creating higher loadability because of the faster recovery, and higher performance level on the competition. Of course, the athletes--as a minimum requirement--need an adequate nutrition (good balanced nourishment), as well, with appropriate application and supplementation of all vitamins and essential minerals.
文摘With the continuous development of the times, cultivating students' cross-cultural consciousness is becoming increasingly urgent. The development of new situation requires that college English teachers should also strengthen students' cultural content education and cultivate students' cross-cultural consciousness, in order to stimulate students' learning interest, optimize students' knowledge structure and improve students' social cultural ability while introducing language knowledge and conducting listening, speaking, reading and writing training for students. Then, how to cultivate students' cross-cultural consciousness in college English teaching? This paper takes it as the theme for analysis, hoping to improve college English teaching quality.
文摘Vocational students generally English foundation is weak, not strong interest in learning how to guide students to learn to think independently, to stimulate students' curiosity, is a series of problems faced by English teachers in vocational college. In this paper, the issues currently facing vocational English education was of, and the corresponding countermeasures. With the development of economic and trade globalization, increasingly strengthened international exchanges. Society is not only high-level personnel to graduate raised higher and higher English proficiency requirements, the ability to develop applications based English Education in Higher Colleges also put forward higher requirements. Vocalional Training Colleges for professional and technical personnel is an important part of our personnel training requirements for rapid economic development of professional and technical personnel in addition to high technical expertise, but also need to understand the professional literature information inquiry, learning and further education. Therefore, we must from the height of the times and the needs of international competition to recognize new opportunities and challenges of English teaching in higher vocational colleges are facing a variety of approaches and strive to improve our English teaching level.
基金supported by National Natural Science Foundation of China (Grant Nos.40976030,41006029),Projects of International Cooperation and Exchanges of National Natural Science Foundation of China (Grant No.41210104029)Knowledge Innovation Program of Chinese Academy of Sciences (Grant No.SQ201114)
文摘Microbes and microbial carbonates in reef-flat and coral community dynamics and submarine geomorphologic features in reef crest and fore reef of Yongxing Island,the Xisha Islands,South China Sea,were studied by means of scuba diving,underwater investigation,and line intercept transect survey.Studies indicate a very high coral mortality with few living corals in the reef flat of Yongxing Island.Moreover,macro algae,sea grass and cyanobacteria are common in reef flat.Microbes and microbially induced carbonates occur in reef flat.Living corals grow mainly in the reef crest and fore reef,but are also declined dramatically.From coast to off shore,the southeast reef flat of Yongxing Island can be divided into beach,inner reef flat,outer reef flat,reef flat front(reef crest and fore reef),and fore-reef slope settings.Sedimentary facies include coast,reef flat,reef crest and fore reef,and fore-reef slope.Reefal carbonate sediments are composed of coral skeletons and framework,coral fragments,bioclasts,and lime mud.With the deterioration of environment and water quality,the coral communities tend to be distributed in the reef crest and fore reef with clean sea water,well circulation and moderate water energy.Reef flat is occupied mainly by the macro algae and Heliopora coerulea communities.The coverage statistics on the reef crest demonstrate that the coverage of Acropora cytherea is more than 28% and represents a dominant species with wave-resistant ecological type.Sedimentary characteristics and geomorphologic features are different between the southeast and northwest reef-flat fronts(reef crest and fore reef) of Yongxing Island.The former shows discontinuously tidal channels in outer reef flat and different dimensional and deep reef ponds in reef crest and fore reef,and the latter presents a typical spur-and-groove system.Microbes(cyanobacteria Lyngbya sp.) occur generally in the inner reef flat and reef ponds of reef crest with restricted water circulation.Widely algae growth indicates a eutrophic environment,and the common microbes on the coral surface in the reef flat and reef ponds also demonstrate eutrophication in seawater and deteriorated water quality.