A cruise of survey in the 2008 Olympics boat-sailing field and adjacent area was carried out on August 28^th, 2003. Preliminarily analysis on the eutrophication status was made on the basis of the content of dissolved...A cruise of survey in the 2008 Olympics boat-sailing field and adjacent area was carried out on August 28^th, 2003. Preliminarily analysis on the eutrophication status was made on the basis of the content of dissolved inorganic nitrogen （DIN）, dissolved inorganic phosphate （PO4-P）, dissolved oxygen（DO） and chemical oxygen demand （COD） as well. The results indicated that in most of the survey area, water quality was in accordance with the 1^st class National Seawater Standard except the average concentration of petroleum hydrocarbon which was beyond the 2^nd class National Seawater Standard. The concentrations of PO4-P as well as DIN were mainly influenced by the runoff or drainage from lands, with a comparatively higher concentration in the coastal areas from the Fushan Bay to the Shilaoren bathing beach. The distribution of eutrophication index （El） showed that part of the survey area was in a state of slight eutrophication The eutrophication was mostly influenced by the land runoff or drainage and related factors. The sea water quality of the Olympic boat-sailing field was fine and just slightly polluted on the whole.展开更多
[Objective] This study aimed to find out effective measures to solve land- scape water eutrophication. [Method] Based on an overview of the urban landscape water characteristics and pollution control situation, taking...[Objective] This study aimed to find out effective measures to solve land- scape water eutrophication. [Method] Based on an overview of the urban landscape water characteristics and pollution control situation, taking an example of the artificial lakes in a campus in Xi＇an, we comparatively analyzed the present pollution situation of Xiuyuan Lake and Mingyuan Lake, and provided controlling measures against eutrophication pollution, as well as restoration measures. [Result] All the indicators （TN, TP, Chl-a, NH3-N, CODMn） of the landscape water quality in both Xiuyuan Lake and Mingyuan Lake went severely beyond the Grade IV standards, presenting a state of heavy eutrophication. Nitrogen, phosphorus and other nutrient salts carried by atmospheric precipitation and its runoff, as well as domestic garbage generated by human activities were primary exogenous pollutants of the artificial lakes. And the release of sediment pollutants was endogenous substances causing deterioration of water quality. [Conclusion] This paper presents some measures to control the pollution of artificial lakes, and provides scientific references for the construction, operation and management of artificial lakes, as well as maintenance of aquatic environment.展开更多
[Objective] Effect of hypoxic water culture on physiological characteristic of water spinach and its hypoxia tolerance were studied.[Method] Water spinach was planted in soil and eutrophication water by means of float...[Objective] Effect of hypoxic water culture on physiological characteristic of water spinach and its hypoxia tolerance were studied.[Method] Water spinach was planted in soil and eutrophication water by means of floating bed,respectively,and the change of physiological indexes including chlorophyll,proline（Pro）,malondialdehyde（MDA）,soluble sugar and soluble protein in water spinach leaves at mature stage was researched,while the hypoxia tolerance of water spinach and the effect of plant density on water spinach growth were discussed in our paper.[Result] In the hypoxic eutrophication water,the content of total chlorophyll,malondialdehyde,soluble sugar and soluble protein in water spinach leaves was lower than that of soil culture,with higher proline content,which showed that water spinach had better tolerance to hypoxic eutrophication water;the higher the plant density,the lower the chlorophyll content in water spinach leaves,and there was no significant effect of plant density on proline and malondialdehyde content,while soluble protein content was higher under high plant density.[Conclusion] The best plant density of water spinach was 66 plants per floating bed with the area of 2 m2,which could provide theoretical basis for the application of water spinach in floating bed.展开更多
[Objective] The objective of this study was to report an improved method for rapid DNA extraction from black-order sediments, without any purification step. [Methods] Sediments in eutrophic lake are complex ecosystems...[Objective] The objective of this study was to report an improved method for rapid DNA extraction from black-order sediments, without any purification step. [Methods] Sediments in eutrophic lake are complex ecosystems and soil microbes involved in anthropogenic nutrient cycling are very important. DNA-based molecular methods offer new tools for characterization of these mixed communities of mi- croorganisms. However, it is very difficult to remove humic substances, heavy met- als that co-existed with genome DNA representing the microbial community directly from these complex systems and can interfere with subsequent genetic analysis. The potassium dichromate solution was firstly used to remove humic substances. [Results] The steps of removing humic substances and DNA extraction were per- formed simultaneously that improved the speed of extraction to approximately 1 hour and the nucleic acids that were obtained with this method did not need to be washed with 70% ethanol and dissolved directly in sterile water for total bacterial 16S rDNA, nosZ gene of denitrifying bacteria, pmoA of methanotrophs, nifH of nitro- gen-fixing bacteria, amoA of ammonia-oxidizing bacteria and ammonia-oxidizing ar- chaea molecular ecology analyses. [Conclusion] This method could provide a plat- form for preparing a fast sediments DNA extraction.展开更多
基金The paper was supported by the National Key Technologies R&D Program(2002BA904B06)Project continuously funded by the Shandong Natural Science Foundation(L2000E01)"Green 0lympics"specialized program of Qingdao Municipal Science and Technology Commission(HAK1203).
文摘A cruise of survey in the 2008 Olympics boat-sailing field and adjacent area was carried out on August 28^th, 2003. Preliminarily analysis on the eutrophication status was made on the basis of the content of dissolved inorganic nitrogen （DIN）, dissolved inorganic phosphate （PO4-P）, dissolved oxygen（DO） and chemical oxygen demand （COD） as well. The results indicated that in most of the survey area, water quality was in accordance with the 1^st class National Seawater Standard except the average concentration of petroleum hydrocarbon which was beyond the 2^nd class National Seawater Standard. The concentrations of PO4-P as well as DIN were mainly influenced by the runoff or drainage from lands, with a comparatively higher concentration in the coastal areas from the Fushan Bay to the Shilaoren bathing beach. The distribution of eutrophication index （El） showed that part of the survey area was in a state of slight eutrophication The eutrophication was mostly influenced by the land runoff or drainage and related factors. The sea water quality of the Olympic boat-sailing field was fine and just slightly polluted on the whole.
基金Supported by National Science Fund for Distinguished Young Scholars of China(41102107)Basic Research Program for Technology Innovation,Chang'an University(CHD2012JC080)~~
文摘[Objective] This study aimed to find out effective measures to solve land- scape water eutrophication. [Method] Based on an overview of the urban landscape water characteristics and pollution control situation, taking an example of the artificial lakes in a campus in Xi＇an, we comparatively analyzed the present pollution situation of Xiuyuan Lake and Mingyuan Lake, and provided controlling measures against eutrophication pollution, as well as restoration measures. [Result] All the indicators （TN, TP, Chl-a, NH3-N, CODMn） of the landscape water quality in both Xiuyuan Lake and Mingyuan Lake went severely beyond the Grade IV standards, presenting a state of heavy eutrophication. Nitrogen, phosphorus and other nutrient salts carried by atmospheric precipitation and its runoff, as well as domestic garbage generated by human activities were primary exogenous pollutants of the artificial lakes. And the release of sediment pollutants was endogenous substances causing deterioration of water quality. [Conclusion] This paper presents some measures to control the pollution of artificial lakes, and provides scientific references for the construction, operation and management of artificial lakes, as well as maintenance of aquatic environment.
基金Supported by Agricultural Science and Technology Achievements Transformation Fund Project of Science and Technology Ministry(2009GB23320484)National Spark Program Project(2010GA760003)~~
文摘[Objective] Effect of hypoxic water culture on physiological characteristic of water spinach and its hypoxia tolerance were studied.[Method] Water spinach was planted in soil and eutrophication water by means of floating bed,respectively,and the change of physiological indexes including chlorophyll,proline（Pro）,malondialdehyde（MDA）,soluble sugar and soluble protein in water spinach leaves at mature stage was researched,while the hypoxia tolerance of water spinach and the effect of plant density on water spinach growth were discussed in our paper.[Result] In the hypoxic eutrophication water,the content of total chlorophyll,malondialdehyde,soluble sugar and soluble protein in water spinach leaves was lower than that of soil culture,with higher proline content,which showed that water spinach had better tolerance to hypoxic eutrophication water;the higher the plant density,the lower the chlorophyll content in water spinach leaves,and there was no significant effect of plant density on proline and malondialdehyde content,while soluble protein content was higher under high plant density.[Conclusion] The best plant density of water spinach was 66 plants per floating bed with the area of 2 m2,which could provide theoretical basis for the application of water spinach in floating bed.
基金Supported by the Recruitment Program of Beifang Univesity of Nationality(Grant No.44/4400302502)~~
文摘[Objective] The objective of this study was to report an improved method for rapid DNA extraction from black-order sediments, without any purification step. [Methods] Sediments in eutrophic lake are complex ecosystems and soil microbes involved in anthropogenic nutrient cycling are very important. DNA-based molecular methods offer new tools for characterization of these mixed communities of mi- croorganisms. However, it is very difficult to remove humic substances, heavy met- als that co-existed with genome DNA representing the microbial community directly from these complex systems and can interfere with subsequent genetic analysis. The potassium dichromate solution was firstly used to remove humic substances. [Results] The steps of removing humic substances and DNA extraction were per- formed simultaneously that improved the speed of extraction to approximately 1 hour and the nucleic acids that were obtained with this method did not need to be washed with 70% ethanol and dissolved directly in sterile water for total bacterial 16S rDNA, nosZ gene of denitrifying bacteria, pmoA of methanotrophs, nifH of nitro- gen-fixing bacteria, amoA of ammonia-oxidizing bacteria and ammonia-oxidizing ar- chaea molecular ecology analyses. [Conclusion] This method could provide a plat- form for preparing a fast sediments DNA extraction.
