Abstract： Stable carbon and nitrogen isotope analysis was used to identify the aquaculture-derived organic matter in the sediment in and around a coastal fish farm in China. Results showed that mean δ13C value in fi...Abstract： Stable carbon and nitrogen isotope analysis was used to identify the aquaculture-derived organic matter in the sediment in and around a coastal fish farm in China. Results showed that mean δ13C value in fish farm area （within 1 O0 m from the edge of cages） and control area （500 m from the edge of cages） was -17.72±1.29/oo and -12.73± 0.380/00, respectively. Mean δ15N value of fish farm area and control area was 6.44 4±0.2%0 and 5.61 4±0.2%0, respectively. The sediment in the fish farm area was characterized by high waste food （47.70%） and faeces （27.71%） ratio, as the distance from the fish cages increased, aquaculture-derived organic matter decreased expontially （y = 97.167e-0.0074x, R2= 0.8481）. The spatial extent of waste dispersal extended to an area up to 400 m.展开更多
Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,ba...Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,based on the expressed sequence tags(EST),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled probe.Of them,101(33.7%) were found to be polymorphic in 48 individuals from 4 populations.Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500.The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,respectively.Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers.BLASTx gave significant hits for 72 of the 101 polymorphic SNP-containing ESTs.Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.展开更多
The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were ...The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were developed with 567 mark- ers （565 microsatellite markers and two SCAR markers）. The parents and F1 progeny （92 individuals） were used as segregating populations. The female map was composed of 480 markers in 21 linkage groups, covering a total of 1388.1 cM, with an average interval 3.06 cM between markers. The male map consisted of 417 markers in 21 linkage groups, spanning 1480.9 cM, with an average interval of 3.75 cM. The female and male maps had 474 and 416 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1522.9 cM for females and 1649.1cM for males. Based on estimations of map length, the female and male maps covered 91.1% and 89.8% of the genome, respectively. Furthermore, two female-specific SCAR mark- ers, f-382 and f-783, were mapped on LG15f （linkage group 15 in female maps）. The present study presents a mid-density genetic linkage map for half-smooth tongue sole. These improved genetic linkage maps may facilitate systematic genome searches to identify quantitative trait loci （QTL）, such as disease resistance, growth and sex-related traits, and are very useful for marker-assisted selection breeding programs for economically important traits in half-smooth tongue sole [Current Zoology 59 （1）： 99-108, 2013].展开更多
文摘Abstract： Stable carbon and nitrogen isotope analysis was used to identify the aquaculture-derived organic matter in the sediment in and around a coastal fish farm in China. Results showed that mean δ13C value in fish farm area （within 1 O0 m from the edge of cages） and control area （500 m from the edge of cages） was -17.72±1.29/oo and -12.73± 0.380/00, respectively. Mean δ15N value of fish farm area and control area was 6.44 4±0.2%0 and 5.61 4±0.2%0, respectively. The sediment in the fish farm area was characterized by high waste food （47.70%） and faeces （27.71%） ratio, as the distance from the fish cages increased, aquaculture-derived organic matter decreased expontially （y = 97.167e-0.0074x, R2= 0.8481）. The spatial extent of waste dispersal extended to an area up to 400 m.
基金the National Natural Science Foundation of China(No.31130054)the National Basic Research Program of China(973 Program,2010CB126406 and 2010CB126402)+2 种基金the National High-Tech R&D Program(863 Program,2012AA10A402 and 2012AA10A405)the National Key Technology R&D Program(2011BAD13B06)the Earmarked Fund for Modern Agro-Industry Technology Research
文摘Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,based on the expressed sequence tags(EST),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled probe.Of them,101(33.7%) were found to be polymorphic in 48 individuals from 4 populations.Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500.The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,respectively.Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers.BLASTx gave significant hits for 72 of the 101 polymorphic SNP-containing ESTs.Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.
文摘The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were developed with 567 mark- ers （565 microsatellite markers and two SCAR markers）. The parents and F1 progeny （92 individuals） were used as segregating populations. The female map was composed of 480 markers in 21 linkage groups, covering a total of 1388.1 cM, with an average interval 3.06 cM between markers. The male map consisted of 417 markers in 21 linkage groups, spanning 1480.9 cM, with an average interval of 3.75 cM. The female and male maps had 474 and 416 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1522.9 cM for females and 1649.1cM for males. Based on estimations of map length, the female and male maps covered 91.1% and 89.8% of the genome, respectively. Furthermore, two female-specific SCAR mark- ers, f-382 and f-783, were mapped on LG15f （linkage group 15 in female maps）. The present study presents a mid-density genetic linkage map for half-smooth tongue sole. These improved genetic linkage maps may facilitate systematic genome searches to identify quantitative trait loci （QTL）, such as disease resistance, growth and sex-related traits, and are very useful for marker-assisted selection breeding programs for economically important traits in half-smooth tongue sole [Current Zoology 59 （1）： 99-108, 2013].
