[Objective]To study the effect of supplies of nitrogen level on spring maize leaf blade carbon metabolism.[Method]In this experiment,field trail and biochemistry analysis were used to study the effect of the diference...[Objective]To study the effect of supplies of nitrogen level on spring maize leaf blade carbon metabolism.[Method]In this experiment,field trail and biochemistry analysis were used to study the effect of the diference-nitrogen level on the content of chlorophyl and carboxylase activity of RuBP and PEP in the leaf of spring maize during main growing period.[Result]Applying proper amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize,insufficient or excessive（N 400 kg/hm^2） of nitrogenous fertilizer has the adverse effect.[Conclusion]In this experiment,applying 300 kg/hm^2 amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize during main growing period.It was important to strengthens the leaf blade photosynthesis ability,promote the yield formation and postpone the decline of leaf blade.展开更多
[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Ten...[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Tenebrio molitor and the contents of crude protein,crude fat,Ca,P,dry matter and crude ash in the half-dry matter of Tenebrio molitor had been studied in the laboratory.[Result] Compared with the recipes of all wheat bran and pure fine fodder,feeding Tenebrio molitor by applying 30%-60% of corn stalks could significantly improve the content of crude protein in the half-dry materials of Tenebrio molitor,but it was detrimental to the improvement in weight of Tenebrio molitor and the accumulation of crude fat.To be specific,the recipe V（50% of corn stalks ＋ 50% of fine fodder） was beneficial to the accumulation of dry matter and crude ash.The recipe VII（70% of corn stalks ＋ 30% of fine fodder） and the recipe VIII（70% of corn stalks treated with Guangda feed leaven ＋ 30% of fine fodder） were beneficial to the accumulation of crude protein,Ca and P.There were differences among the influences of corn stalks in different treatments on the crude protein and other nutrition indicators of Tenebrio molitor.[Conclusion] Appropriately applying corn stalks can improve the accumulation of the crude protein,crude ash,dry matter,Ca and P of Tenebrio molitor but was detrimental to the growth and development of Tenebrio molitor and the accumulation of crude fat.展开更多
[Objective] The aim was to provide scientific basis for improving the middle and low yielding fields fertility and farmland productivity. [Method] A field experiment was carried out to study the effects of different m...[Objective] The aim was to provide scientific basis for improving the middle and low yielding fields fertility and farmland productivity. [Method] A field experiment was carried out to study the effects of different management practices （including nutrient management systems and cultivation methods） on crop yield and soil fertility in winter wheat/summer maize rotation system. [Result] The crop yield in the treatment of the high yield and high efficiency system was remarkably higher than farmer conventional management practice. After five crop seasons experiment, the contents of soil organic matter for high yield and high efficiency system increased 2.72-3.01 g/kg, and that of soil total nitrogen increased 0.12-0.16 g/kg, the soil Olsen-P increased 5.2 mg/kg and the soil available K （NH4OAC-K） increased about 37.8 mg/kg. [Conclusion] Considering the yield and soil fertility comprehensively, the management system of high yield and high efficiency could effectively increase the crop yield and improve the soil fertility.展开更多
[Objective] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage,so as to prov...[Objective] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage,so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [Method] Taking the stem segments of E. tirucalli as explants,the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [Result] The optimum induction medium of germination culture was 1/2MS＋NAA 0.02 mg/L＋6-BA 1.0 mg/L,with differentiation rate of 89.7%; the best subculture medium was 1/2MS＋NAA 0.02 mg/L＋6-BA 0.60 mg/L＋AD 3.0 mg/L,with multiplication coefficient of 5.70; the optimum rooting culture medium was 1/2MS＋NAA 0.40 mg/L＋IBA 0.4 mg/L,with rooting rate of 100% and transplanting survival rate of 80%. [Conclusion] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.展开更多
A modified glass bead compartment cultivation system was used tocompare some chemical and biolog- ical properties of the twoarbuscular mycorrhizal (AM) fungi Glmomus mosseae and Glomusversiforme using maize (Zea mays)...A modified glass bead compartment cultivation system was used tocompare some chemical and biolog- ical properties of the twoarbuscular mycorrhizal (AM) fungi Glmomus mosseae and Glomusversiforme using maize (Zea mays) as the host plant with four addedlevels of available phosphorus (P). The proportion of host plant rootlength infected was determined t harvest. Shoot and root yields andnutrient concentra- tions were determined, together with the nutrientconcentrations in the AM fungal external mycelium. The morphology ofvarious mycorrhizal structures of the two AM fungi was also comparedby microscopic obser- vation.展开更多
Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertiliza...Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertilization mechanisms of plants. In this study, we developed a bovine serum albumin (BSA)-mediated fusion system for the in vitro fusion of maize gametes. The in vitro fusion of the isolated single egg cell and sperm cell of maize was observed microscopically in the BSA solution and the fertilized egg cell showed normal cell wall regeneration and nuclear division. The effects of the BSA concentration, pH value and calcium level on the efficiency of the maize gamete fusion were also assessed. BSA concentration and pH value did significantly affect the efficiency of the gamete fusion. Calcium was not necessary for the gamete fusion when BSA was present. The optimal solution for the gamete fusion contained 0.1% BSA, pH 6.0. The fusion frequency was as high as 96.7% in that optimal solution. This new in vitro fertilization system offers an alterna- tive tool for the in vitro study of fertilization mechanisms with much simpler manipulating procedure than PEG system, and it will be especially useful for the in vitro study of the calcium dynamics during plant fertilization.展开更多
[Objective] The aim was to research rational fertilization of corn in Yuanzhou District, Guyuan, Ningxia Hui Autonomous Region. [Method] Nutrient con- tents in soils in Yuanzhou District were measured with conventiona...[Objective] The aim was to research rational fertilization of corn in Yuanzhou District, Guyuan, Ningxia Hui Autonomous Region. [Method] Nutrient con- tents in soils in Yuanzhou District were measured with conventional analysis method and the suitable fertilization scheme of corn growth was proposed based on fertiliz- er-application rule of corn growth and nutrient balance. [Result] Soil in farmlands in Yuanzhou District was of alkalinity; organic matter, N, P and Zn were shortage; S was extremely insufficient; Fe and Mn were moderate; K was abundant; Cu would satisfy crop growth, [Conclusion] The research provides references for yield increase, reduction of chemical fertilizer and related pollution for corn growth.展开更多
基金Supported by National Key Technology R & D Program during 11th Five-years Plan(2006BAD02A10)~~
文摘[Objective]To study the effect of supplies of nitrogen level on spring maize leaf blade carbon metabolism.[Method]In this experiment,field trail and biochemistry analysis were used to study the effect of the diference-nitrogen level on the content of chlorophyl and carboxylase activity of RuBP and PEP in the leaf of spring maize during main growing period.[Result]Applying proper amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize,insufficient or excessive（N 400 kg/hm^2） of nitrogenous fertilizer has the adverse effect.[Conclusion]In this experiment,applying 300 kg/hm^2 amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize during main growing period.It was important to strengthens the leaf blade photosynthesis ability,promote the yield formation and postpone the decline of leaf blade.
基金Supported by National Special Fund for Scientific Research in Public Welfare Trade(Agriculture)(200803033-B0602-01)Project of Hebei Province Scientific and Technological Department(10221019)+1 种基金Project of Qinghuangdao City Science and Technology Burea of Hebei Province(201001A152)Foundation of Education Committee of Hebei Province(2003308)~~
文摘[Objective] The study aimed at reducing the economic cost in feeding Tenebrio molitor and improving the utilization ratio of corn stalks.[Method] The effects of different proportions of corn stalks on the weigh of Tenebrio molitor and the contents of crude protein,crude fat,Ca,P,dry matter and crude ash in the half-dry matter of Tenebrio molitor had been studied in the laboratory.[Result] Compared with the recipes of all wheat bran and pure fine fodder,feeding Tenebrio molitor by applying 30%-60% of corn stalks could significantly improve the content of crude protein in the half-dry materials of Tenebrio molitor,but it was detrimental to the improvement in weight of Tenebrio molitor and the accumulation of crude fat.To be specific,the recipe V（50% of corn stalks ＋ 50% of fine fodder） was beneficial to the accumulation of dry matter and crude ash.The recipe VII（70% of corn stalks ＋ 30% of fine fodder） and the recipe VIII（70% of corn stalks treated with Guangda feed leaven ＋ 30% of fine fodder） were beneficial to the accumulation of crude protein,Ca and P.There were differences among the influences of corn stalks in different treatments on the crude protein and other nutrition indicators of Tenebrio molitor.[Conclusion] Appropriately applying corn stalks can improve the accumulation of the crude protein,crude ash,dry matter,Ca and P of Tenebrio molitor but was detrimental to the growth and development of Tenebrio molitor and the accumulation of crude fat.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201103003)~~
文摘[Objective] The aim was to provide scientific basis for improving the middle and low yielding fields fertility and farmland productivity. [Method] A field experiment was carried out to study the effects of different management practices （including nutrient management systems and cultivation methods） on crop yield and soil fertility in winter wheat/summer maize rotation system. [Result] The crop yield in the treatment of the high yield and high efficiency system was remarkably higher than farmer conventional management practice. After five crop seasons experiment, the contents of soil organic matter for high yield and high efficiency system increased 2.72-3.01 g/kg, and that of soil total nitrogen increased 0.12-0.16 g/kg, the soil Olsen-P increased 5.2 mg/kg and the soil available K （NH4OAC-K） increased about 37.8 mg/kg. [Conclusion] Considering the yield and soil fertility comprehensively, the management system of high yield and high efficiency could effectively increase the crop yield and improve the soil fertility.
基金Supported by Key Technologies R &D Program of Guangdong Province (2009B0203030092006B20201007)~~
文摘[Objective] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage,so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [Method] Taking the stem segments of E. tirucalli as explants,the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [Result] The optimum induction medium of germination culture was 1/2MS＋NAA 0.02 mg/L＋6-BA 1.0 mg/L,with differentiation rate of 89.7%; the best subculture medium was 1/2MS＋NAA 0.02 mg/L＋6-BA 0.60 mg/L＋AD 3.0 mg/L,with multiplication coefficient of 5.70; the optimum rooting culture medium was 1/2MS＋NAA 0.40 mg/L＋IBA 0.4 mg/L,with rooting rate of 100% and transplanting survival rate of 80%. [Conclusion] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.
基金National Key Basic Research Support Foundation(NKBRSF)of China(No.G1999011807) the Joint Open Laboratory of Soil and Environment between the Institute of Soil Science of the Chinese Academy ot Sciences and Hong Kong Baptist University. 2Corresp
文摘A modified glass bead compartment cultivation system was used tocompare some chemical and biolog- ical properties of the twoarbuscular mycorrhizal (AM) fungi Glmomus mosseae and Glomusversiforme using maize (Zea mays) as the host plant with four addedlevels of available phosphorus (P). The proportion of host plant rootlength infected was determined t harvest. Shoot and root yields andnutrient concentra- tions were determined, together with the nutrientconcentrations in the AM fungal external mycelium. The morphology ofvarious mycorrhizal structures of the two AM fungi was also comparedby microscopic obser- vation.
基金This research was supported by the National Natural Science Foundation of China(30370743,90408002)National Science Found for Distin-guished Young Scholars of China(30225006)Supported by Program for Changjiang Scholars and Innovative Re-search Team in University(PCSIRT).
文摘Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertilization mechanisms of plants. In this study, we developed a bovine serum albumin (BSA)-mediated fusion system for the in vitro fusion of maize gametes. The in vitro fusion of the isolated single egg cell and sperm cell of maize was observed microscopically in the BSA solution and the fertilized egg cell showed normal cell wall regeneration and nuclear division. The effects of the BSA concentration, pH value and calcium level on the efficiency of the maize gamete fusion were also assessed. BSA concentration and pH value did significantly affect the efficiency of the gamete fusion. Calcium was not necessary for the gamete fusion when BSA was present. The optimal solution for the gamete fusion contained 0.1% BSA, pH 6.0. The fusion frequency was as high as 96.7% in that optimal solution. This new in vitro fertilization system offers an alterna- tive tool for the in vitro study of fertilization mechanisms with much simpler manipulating procedure than PEG system, and it will be especially useful for the in vitro study of the calcium dynamics during plant fertilization.
基金Supported by Programs for Science and Technology Development in Ningxia Hui Autonomous Region(NKJ2010-168-177)~~
文摘[Objective] The aim was to research rational fertilization of corn in Yuanzhou District, Guyuan, Ningxia Hui Autonomous Region. [Method] Nutrient con- tents in soils in Yuanzhou District were measured with conventional analysis method and the suitable fertilization scheme of corn growth was proposed based on fertiliz- er-application rule of corn growth and nutrient balance. [Result] Soil in farmlands in Yuanzhou District was of alkalinity; organic matter, N, P and Zn were shortage; S was extremely insufficient; Fe and Mn were moderate; K was abundant; Cu would satisfy crop growth, [Conclusion] The research provides references for yield increase, reduction of chemical fertilizer and related pollution for corn growth.
